A5014829644- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- RNA modifications and cancer
- Circular RNAs in diseases
- Antimicrobial Resistance in Staphylococcus
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Oral microbiology and periodontitis research
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- Gut microbiota and health
- Animal Genetics and Reproduction
- Herpesvirus Infections and Treatments
- Diet and metabolism studies
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Bone fractures and treatments
- Ubiquitin and proteasome pathways
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2012-2024
Beijing Institute of Education
2011-2024
Academy of Military Medical Sciences
2004-2020
Institute of Physiology and Basic Medicine
2009-2018
Sun Yat-sen University
2013
Qilu Hospital of Shandong University
2012
Beijing Advanced Sciences and Innovation Center
2011
Chinese PLA General Hospital
2010
Northwest Normal University
2010
Columbia University
2010
In this article, a panel of ssDNA aptamers specific to Staphylococcus aureus was obtained by whole bacterium-based SELEX procedure and applied probing S. aureus. After several rounds selection with as the target Streptococcus epidermidis counter targets, highly enriched oligonucleic acid pool sequenced then grouped under different families on basis homology primary sequence similarity secondary structure. Eleven sequences from were selected for further characterization confocal imaging flow...
This study proposes a facile method for synthesis of Au-coated magnetic nanoparticles (AuMNPs) core/shell nanocomposites with nanoscale rough surfaces. MnFe2O4 (NPs) were first modified uniform polyethylenimine layer (2 nm) through self-assembly under sonication. The negatively charged Au seeds then adsorbed on the surface NPs electrostatic interaction shell formation. Our newly developed sonochemically assisted hydroxylamine seeding growth was used to grow gold into large (AuNPs) form...
A magnetically assisted surface-enhanced Raman scattering (SERS) biosensor for single-cell detection of S. aureus on the basis aptamer recognition is reported first time. The consists two basic elements including a SERS substrate (Ag-coated magnetic nanoparticles, AgMNPs) and novel tag (AuNR–DTNB@Ag–DTNB core–shell plasmonic NPs or DTNB-labeled inside-and-outside NPs, DioPNPs). Uniform, monodisperse, superparamagnetic AgMNPs with favorable activity responsiveness are synthesized by using...
We report a new in situ tissue slide-based SELEX strategy targeting neoplastic tissues from breast cancer patients. The methodology, using the molecular differences between clinical specimens, can evolve aptamers to all fractions of tissue. may be used as probes for pathological diagnosis and tumour imaging, also reveal that are responsible diseases. specific were enriched by unequal length strand PCR employing structured (-) primer. After 12 rounds selection, paraffin sections infiltrating...
Vasorin (VASN) is a type I transmembrane protein that plays important roles in tumor development and vasculogenesis.In this paper, we showed VASN could be key mediator of communication between cells endothelial cells.We confirmed for the first time HepG2-derived can transferred to human umbilical vein (HUVECs) via receptor mediated endocytosis exosomes, at least part through HSPGs.The containing exosomes promote migration recipient HUVECs cells.Our results identify novel pathway by which...
Aptamers which specifically recognize cytotoxin ricin were successfully selected using the two different in vitro selection methods. One method was used to isolate aptamers by affinity chromatography. Another method, named CE-SELEX, carried out CE as a separation approach. The high efficiency of evidently improved rate enrichment and obviously shortened rounds, with near 87.2% binding just after fourth round selection. A3, C1, C5, derived from methods, found possess specificity for Kd values...
The Hfq protein is reported to be an RNA chaperone, which involved in the stress response and virulence of several pathogens. In E. coli, can mediate interaction between some sRNAs their target mRNAs. But it controversial whether plays important role S. aureus. this study, we found that deletion hfq gene aureus 8325-4 increase surface carotenoid pigments. mutant was more resistant oxidative but pathogenicity reduced. We reveal detected only strains. Using microarray qRT-PCR, identified 116...
In this study, we investigated the feasibility and safety of intravenous transplantation allogeneic bone marrow mesenchymal stem cells (MSCs) for femoral head repair, observed migration distribution MSCs in hosts. were labeled with green fluorescent protein (GFP) vitro injected into nude mice via vena caudalis, was dynamically monitored at 0, 6, 24, 48, 72 96 h after transplantation. Two weeks establishment a rabbit model necrosis, GFP these rabbits ear vein, immunological rejection graft...
MicroRNAs (miRNAs) play important roles in tumorigenesis and metastasis. In this study, we investigated miR-200b expression endometrial adenocarcinomas normal adjacent tissues found that is more highly expressed cancer than tissues. A novel target of miR-200b, tissue inhibitor metalloproteinase 2 (TIMP2), was predicted using a bioinformatics approach confirmed human cell line HEC-1A cells by luciferase assay, quantitative real-time polymerase chain reaction, western blotting, enzyme-linked...
MicroRNAs (miRNAs) are a class of non-coding small RNAs that consist ∼22 nt and involved in several biological processes by regulating target gene expression. MiR-138 has many functions is often downregulated cancers. Our results showed overexpression miR-138 RMND5A (required for meiotic nuclear division 5 homolog A) reduced Exportin-5 stability, which decreased levels pre-miRNA export HeLa cells. We also found could significantly inhibit cell migration targeting RMND5A. study therefore...
Many studies have shown that the quantity and dynamics of circulating tumor cells (CTCs) in peripheral blood patients afflicted with solid tumours great relevance therapeutic efficacy prognosis. Different methods based on various strategies been developed to isolate identify CTCs, but their needs be improved because rarity complexity CTCs. This study was designed examine possibility using a SELEX aptamer (BC-15) as probe rare CTCs out background nucleated cells. Aptamer BC-15 selected from...
In this study, the CAR-like multivalent aptamer nanoparticles (X-polymers) were assembled with dimer of murine CD28 RNA (CD28Apt7), tetramer CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) (Del60), and a folic acid labeled ssDNA fragment in stable nucleic three-way junction scaffold (3WJ). Results showed that X-polymers could recognize both mCD28 mCTLA-4 molecules. Confocal imaging flow cytometry assays target T cells B16 vitro. With first costimulatory signals provided by CD3...
Staphylococcus aureus is a gram-positive pathogen responsible for wide variety of diseases. RNAIII the key effector accessory gene regulator (agr) system. It regulatory RNA (514 nucleotides long) that acts at both transcription and translation level to regulate production numerous toxins, enzymes cell surface proteins. Here, we reveal map (major histocompatibility complex class II analogous protein) positively regulated by RNAIII. Our further study indicates 108–135 nt fragment as an...