Although hyperandrogenism is an important condition and considered the possible pathogenesis behind polycystic ovary syndrome (PCOS), data supporting this still scarce. We sought to determine whether or not prenatal androgen exposure leads PCOS cellular mechanisms involved. To induce exposure, pregnant rats were treated with daily subcutaneous injections of free testosterone (T) dihydrotestosterone (DHT) from embryonic days 16 19, their female offspring studied as adults. The mRNA expression...

ABSTRACT: Human semen cryopreservation in the clinical management of male infertility is complicated by cryodamage to spermatozoa. We aimed clarify full pattern and evaluate protective effects ascorbate catalase on cryopreserved Semen samples were collected from 30 fertile males. Each sample was divided into 6 groups: fresh semen, without treatment, with (300 or 600 μM) (200 400 IU/mL). Spermatozoa examined for their viability, motility, reactive oxygen species (ROS) generation,...

This study was designed to evaluate the effects of semen processing on generation intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) in spermatozoa, develop reliable indexes for evaluation sperm quality during preparation. Swim-up density gradient centrifugation methods were used separate oligoasthenoteratozoospermia (OAT), leucocytospermia (LC) normozoospermia groups. Levels ROS MMP measured by flow cytometry. Before preparation, patients with abnormal...

Human sperm cryopreservation for assisted reproduction is compromised by ROS-induced cryodamage. Our previous model study in which mouse were treated with H2O2 to simulate DNA-damage caused cryopreservation-induced ROS have discovered that embryos fertilized showed a delay cleavage might be associated cell cycle arrest. The checkpoint pathway underlying the remained elusive. Moreover, our also indicated γH2AX, repair marker, was functional similarly fertilized, but completeness and...

Background Cryopreservation of human semen for assisted reproduction is complicated by cryodamage to spermatozoa caused excessive reactive oxygen species (ROS) generation. Methods and Findings We used exogenous ROS (H2O2) simulate cryopreservation examined DNA damage repair in embryos fertilized with sperm H2O2-induced damage. Sperm samples were collected from epididymis adult male KM mice treated capacitation medium (containing 0, 0.1, 0.5 1 mM H2O2) or cryopreservation. The model...

Abstract Objectives A high rate of chromosome aneuploidy is exhibited in vitro fertilization (IVF)‐derived embryos. Our previous experiments suggested that reactive oxygen species (ROS) can activate Mad2, a key protein the spindle assembly checkpoint (SAC), and delay first mitotic, providing time to prevent formation embryonic aneuploidy. We aimed determine whether mitotic kinase Aurora B was involved SAC function IVF‐derived Materials Methods analysed repair during embryo pre‐implantation...

Cryopreservation can induce damage in human spermatozoa through reactive oxygen species (ROS) generation. To reduce the potential risk of oxidative stress-induced sperm DNA damage, addition different epigallocatechin-3-gallate (EGCG) concentrations were performed to determine optimum concentration which was beneficial for IVF outcome both fresh and frozen-thawed sperm. Next, mouse model exhibiting by exogenously treating with H2O2 but overcoming low fertilization rate used investigate effect...

In zygotes, DNA damage delays the first cleavage to enable repair. Our previous study found that 0.03 mM hydrogen peroxide (H2O2) was minimum concentration required for induction of oxidative in mouse zygotes and this represented most similar situation clinical phenomenon. study, we quantified rates cells blastocysts at different developmental stages, followed by immunofluorescence detect activation γ-H2A histone family member X (a marker damage) confirm induced H2O2-treated zygotes....

Gonadotrophin‑releasing hormone (GnRH) agonists (GnRHa) have been widely used to induce a state of downregulation for in vitro fertilization, and its direct effects on the pituitary are well known. However, GnRHa expression anti‑mullerian (AMH) by follicles varying stages vivo remain be fully elucidated. In present study 84 cyclic mice were randomly divided equally into four groups three as control group. The levels AMH between days 0 7 following administration quantified using...

The process of ovarian cryopreservation and transplantation is the only feasible fertility preservation method for prepubertal girls female patients with cancer who cannot delay radiotherapy chemotherapy. However, basic research on this technique lacking. To better understand function oocyte quality after tissue (OT) transplantation, we characterised appearance, angiogenesis, endocrine grafts in a murine model; mitochondrial DNA damage oocytes isolated from OT; development embryos vitro...

Abstract Background Professional legislation and ethics guidelines for posthumous assisted reproduction (PAR) are lacking in China. This study aims to measure the attitudes of general public, IVF couples, reproductive technology (ART) practitioners toward PAR Methods A multi-dimensional survey was designed, electronic questionnaires were used. General demographic data, viewpoints, PAR, interactive ability predict partner’s attitude legal attributes rights disposal embryos evaluated. Results...

Abstract Background Professional legislation and ethics guidelines for posthumous assisted reproduction (PAR) are lacking in China. This study aims to measure the attitudes of general public, IVF couples, ART practitioners toward PAR Methods A multi-dimensional survey was designed, electronic questionnaires were used. General demographic data, reproductive viewpoints, PAR, interactive predictive legal attribute disposition right embryos evaluated. Results The found that traditional Chinese...