A5001801164- Pancreatitis Pathology and Treatment
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Liver physiology and pathology
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Plant Genetic and Mutation Studies
- Folate and B Vitamins Research
- Plant tissue culture and regeneration
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Phagocytosis and Immune Regulation
- RNA and protein synthesis mechanisms
- Genetic factors in colorectal cancer
- Plant Molecular Biology Research
- Pancreatic and Hepatic Oncology Research
- Insect Resistance and Genetics
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- Slime Mold and Myxomycetes Research
- Diabetes and associated disorders
Universitat de València
2014-2024
INCLIVA Health Research Institute
2014-2024
Icahn School of Medicine at Mount Sinai
2011
Universität Innsbruck
1991-1993
Innsbruck Medical University
1992-1993
The epigenetic identity of oligodendrocytes is modulated by posttranslational modifications histones. Acetylation histone H3 results from the balance between activity acetyltransferases (HATs) and deacetylases modulates transcriptional activation. We have previously shown that, in rodents, deacetylation favors oligodendrocyte differentiation, whereas acetylation associated with increased levels inhibitors differentiation. Here, we report, humans brains, a shift toward white matter frontal...
Axonal damage is widely accepted as a major cause of permanent functional disability in Multiple Sclerosis (MS). In relapsing-remitting MS, there possibility remyelination by myelin producing cells and restoration neurological function. The purpose this study was to delineate the pathophysiological mechanisms underpinning axonal injury through hitherto unknown factors present cerebrospinal fluid (CSF) that may regulate damage, remyelinate axon make recovery possible. We employed primary...
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes synthesis of S-adenosylmethionine (AdoMet), main donor methyl groups in cell. In mammals MAT product two genes, MAT1A and MAT2A. expressed only mature liver whereas fetal hepatocytes, extrahepatic tissues cancer cells express The mechanisms behind tissue differentiation state specific expression are not known. present work we examined promoter methylation status by means sensitive restriction analysis. Our data indicate...
SPECIFIC AIMSWe have studied the molecular mechanisms and mediators behind induction of methionine adenosyltransferase 2 A (MAT2A) gene expression in regenerating rat liver after partial he...
Background Human early growth response-1 (EGR1) is a member of the zing-finger family transcription factors induced by range molecular and environmental stimuli including epidermal factor (EGF). In recently published paper we demonstrated that integrin/EGFR cross-talk was required for Egr1 expression through activation Erk1/2 PI3K/Akt/Forkhead pathways. EGR1 activity stability can be influenced many different post-translational modifications such as acetylation, phosphorylation,...
Abstract Chromatin remodeling seems to regulate the patterns of proinflammatory genes. Our aim was provide new insights into epigenetic mechanisms that control transcriptional activation early- and late-response genes in initiation development severe acute pancreatitis as a model inflammation. changes were studied by chromatin immunoprecipitation analysis, nucleosome positioning, determination histone modifications promoters vivo course taurocholate-induced necrotizing rats vitro rat...
Mono Q ion exchange high performance liquid chromatography (HPLC) reveals that the main histone deacetylase activity (HD1) of germinating Zea mays embryos consists multiple enzyme forms. Chromatography HD1 after treatment with alkaline phosphatase yields two distinct forms (HD1-A, HD1-B). The same is true for a total cell extract. One these (HD1-A) subject to phosphorylation, which causes change in substrate specificity enzyme, as shown HPLC-purified individual core species; H2A increases...
Multiple forms of histone acetyltransferases and deacetylases, which have been separated characterized in the accompanying manuscript (Lopez-Rodas, G., Georgieva, E. I., Sendra, R., Loidl, P. (1991) J. Biol. Chem. 266, 18745-18750), together with vivo acetate incorporation, were studied during germination Zea mays embryos. Total acetyltransferase activity increases two maxima at 40 72 h after start germination. This fluctuation is mainly due to cytoplasmic B-enzyme predominantly acetylates...
Mitogen-activated protein kinases (MAPKs) are considered major signal transducers early during the development of acute pancreatitis. Pentoxifylline is a phosphodiesterase inhibitor with marked anti-inflammatory properties through blockade extracellular regulated kinase (ERK) phosphorylation and tumor necrosis factor alpha production. Our aim was to elucidate mechanism action pentoxifylline as an agent in Necrotizing pancreatitis induced by taurocholate rats taurocholate-treated AR42J acinar...
DEAE-Sepharose chromatography of extracts from Zea mays meristematic cells revealed multiple histone acetyltransferase and deacetylase enzyme forms. An improved method for nuclear isolation allowed us to discriminate cytoplasmic enzymes. Two acetyltransferases, A1 A2, a B-enzyme two deacetylases, HD1 HD2, have been identified. The specificity the different forms has studied in an vitro system, using chicken erythrocyte histones as substrate. B is predominant enzyme, which acetylates mainly...
The Id (inhibitor of DNA binding or inhibitor differentiation) helix–loop–helix proteins are involved in the regulation cell growth, differentiation and cancer. fact that molecular mechanisms liver regeneration not completely understood prompted us to study fate Id2 proliferating liver. increases after partial hepatectomy, following early induction its gene. Co-immunoprecipitation shows forms a complex with E2F4, p130 mSin3A quiescent all these components present at c-myc promoter as shown...
Background Modifications on specific histone residues and DNA methylation play an essential role in lineage choice cellular reprogramming. We have previously shown that modifications or combinatorial codes of transcription factors (TFs) are critical for the differentiation multipotential progenitors into myelinating oligodendrocytes. In this study we asked whether combining global manipulation acetylation together with expression oligodendrocyte- TFs, was sufficient to switch identity...
Abstract The finding of novel molecular markers for prediction or prognosis invasiveness in colorectal cancer (CRC) constitutes an appealing challenge. Here we show the up-regulation EPDR1 a prospective cohort 101 CRC patients, cDNA array 43 patients and silico analyses. encodes protein related to ependymins, family glycoproteins involved intercellular contacts. A thorough statistical model allowed us conclude that gene is significantly up-regulated tumour tissues when compared with normal...