A5019310092- Occupational and environmental lung diseases
- Lipid metabolism and biosynthesis
- Cell Adhesion Molecules Research
- Pleural and Pulmonary Diseases
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Skin and Cellular Biology Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- HER2/EGFR in Cancer Research
- Adipose Tissue and Metabolism
- Fibroblast Growth Factor Research
- Estrogen and related hormone effects
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Blood properties and coagulation
- Cancer Research and Treatments
- Medical Imaging and Pathology Studies
- Hops Chemistry and Applications
- Neuroblastoma Research and Treatments
- Connective tissue disorders research
- Spaceflight effects on biology
- Electromagnetic Fields and Biological Effects
- Cancer Cells and Metastasis
- Protease and Inhibitor Mechanisms
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2015-2024
University of Turin
1998-2008
University of Pavia
1988-2004
Ricerca sul Sistema Energetico (Italy)
2000
University of Brescia
1990-1995
Integrin-mediated cell adhesion cooperates with growth factor receptors in the control of proliferation, survival, and migration. One mechanism to explain these synergistic effects is ability integrins induce phosphorylation receptors, for instance epidermal (EGF) receptor. Here we define some aspects molecular mechanisms regulating integrin-dependent EGF receptor phosphorylation. We show that early phases associate on membrane a macromolecular complex including adaptor protein p130Cas c-Src...
DS (Down's syndrome) is the most common human aneuploidy associated with mental retardation and early neurodegeneration. Mitochondrial dysfunction has emerged as a crucial factor in pathogenesis of numerous neurological disorders including DS, but cause mitochondrial damage remains elusive. In present study, we identified new molecular events involved which could play role pathogenesis. We analysed respiratory chain function DS-HSFs syndrome foetal skin fibroblasts; fibroblasts chromosome 21...
Steroid hormones bind to their receptors and trans-activate target genes. Rapid non-genomic action of steroid has been proposed in addition the one at genomic level. Estrogen described activate c-Src kinase this activation shown be responsible for estrogen-dependent mitogenicity. A major substrate activity is cytoskeletal protein p130Cas, originally identified v-Src-transformed cells. We show that human breast carcinoma T47D cells, upon estrogen treatment, p130Cas rapidly transiently...
Malignant pleural mesothelioma is an asbestos-related neoplasm with poor prognosis, refractory to current therapies, the incidence of which expected increase in next decades. Female gender was identified as a positive prognostic factor among other clinical and biological markers for malignant mesothelioma, yet role estrogen receptors (ERs) has not been studied. Our goal investigate ERs expression assess whether their correlates prognosis. Immunohistochemical analysis revealed intense nuclear...
Abstract The early gene growth response (Egr‐1), a broadly expressed member of the zing‐finger family transcription factors, is induced in many cell types by variety and differentiation stimuli, including epidermal factor (EGF). Here we demonstrate that Egr‐1 expression mainly regulated integrin‐mediated adhesion. Integrin‐dependent adhesion plays dual role regulation, either being sufficient “per se” to induce Egr‐1, or required for EGF‐dependent which occurs only adherent cells not...
Background The role of estrogen and receptors in oncogenesis has been investigated various malignancies. Recently our group identified receptor beta (ERβ) expression as an independent prognostic factor the progression human Malignant Pleural Mesothelioma (MMe), but underlying mechanism by which ERβ tumors determines clinical outcome remains largely unknown. This study is aimed at investigating molecular mechanisms action MMe cells disclosing potential translational implications these...
Estrogen receptor (ER) β has growth inhibitory and chemo drug potentiating effect on ovarian cancer cells. We studied the dependence of ERβ function presence KDM6B SIRT1 in human cells vitro. Activation with subtype-selective agonist KB9520 resulted significant inhibition cell growth. KB9520-activated had an additive combination cisplatin paclitaxel, respectively. Loss expression a negative as ligand-dependent inhibitor In contrast, loss or deacetylase activity restored ligand-activated...
Abstract Background Chanarin-Dorfman syndrome (CDS) is a rare autosomal recessive disorder characterized by nonbullous congenital ichthyosiform erythroderma (NCIE) and an intracellular accumulation of triacylglycerol (TG) droplets in most tissues. The clinical phenotype involves multiple organs systems, including liver, eyes, ears, skeletal muscle central nervous system (CNS). Mutations ABHD5/CGI58 gene are associated with CDS. Methods Eight CDS patients belonging to six different families...
Background Human early growth response-1 (EGR1) is a member of the zing-finger family transcription factors induced by range molecular and environmental stimuli including epidermal factor (EGF). In recently published paper we demonstrated that integrin/EGFR cross-talk was required for Egr1 expression through activation Erk1/2 PI3K/Akt/Forkhead pathways. EGR1 activity stability can be influenced many different post-translational modifications such as acetylation, phosphorylation,...
Neutral lipid storage disease with myopathy (NLSD-M) is a rare autosomal recessive disorder characterised by an abnormal accumulation of triacylglycerol into cytoplasmic droplets (LDs). NLSD-M patients are mainly affected progressive myopathy, cardiomyopathy and hepatomegaly. Mutations in the PNPLA2 gene cause variable phenotypes NLSD-M. codes for adipose triglyceride lipase (ATGL), enzyme that hydrolyses fatty acids from triacylglycerol. This report outlines clinical genetic findings...
Estrogen receptor (ER)-β has been shown to possess a tumor suppressive effect, and is potential target for cancer therapy. Using gene-expression meta-analysis of human malignant pleural mesothelioma, we identified an ESR2 (ERβ coding gene) signature. High expression was strongly associated with low succinate dehydrogenase B (SDHB) (which encodes mitochondrial respiratory chain complex II subunit) expression. We demonstrate that SDHB loss induced expression, activated ERβ, by over-expression...
Neutral lipid storage disease with myopathy (NLSDM) presents skeletal muscle and severe dilated cardiomyopathy in nearly 40% of cases. NLSDM is caused by mutations the PNPLA2 gene, which encodes adipose triglyceride lipase (ATGL). Here we report clinical genetic findings a patient carrying two novel (c.696+4A>G c.553_565delGTCCCCCTTCTCG). She presented at age 39 right upper limb abduction weakness slowly progressing over years asymmetric involvement proximal lower muscles. Cardiological...
Abstract Malignant Pleural Mesothelioma ( MMe ) is a rare but increasingly prevalent, highly aggressive cancer with poor prognosis. The aetiology of essentially function previous exposure to asbestos fibres, which are considered be an early‐stage carcinogen. Asbestos toxic human mesothelial cells HMCs ), that activate the nuclear enzyme poly( ADP ‐ribose) polymerase‐1 PARP1 repair DNA . targeting showing considerable potential for delivering selective tumour cell kill while sparing normal...
PI3K/AKT signalling pathway is aberrantly active and plays a critical role for cell cycle progression of human malignant pleural mesothelioma (MMe) cells. AKT one the important cellular targets perifosine, novel bio-available alkylphospholipid that has displayed significant anti-proliferative activity in vitro vivo several tumour model systems currently being tested clinical trials.We Perifosine on mesothelial cells different lines, order to provide evidence its efficacy as single agent...
Deficiency of electron transfer flavoprotein dehydrogenase (ETFDH) is associated with multiple acyl-CoA deficiency (MADD). This disorder an autosomal recessive lipid storage myopathy (LSM) that exhibits a wide range clinical features, including myopathy, weakness and multisystem dysfunctions. Many patients late onset MADD improve when treated riboflavin are also referred to as RR-MADD (riboflavin-responsive Acyl-CoA disorder). In this study, we report the genetic characterization novel...
Fast and simple: the complexity of steroids can be mimicked by two phenol rings linked together easiest reactions: click [3+2] azide-alkyne cycloaddition. Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2452/2007/z600192_s.pdf or from author. Please note: The publisher not responsible content functionality any supporting supplied authors. Any queries (other than missing content) should directed to corresponding author article.
Abstract Malignant pleural mesothelioma (MPM) is an aggressive tumor linked to environmental/occupational exposure asbestos, characterized by the presence of significant areas hypoxia. In this study, we firstly explored expression and role transglutaminase 2 (TG2) in MPM cell adaptation We demonstrated that cells derived from biphasic express full-length TG2 variant at higher levels than epithelioid normal mesothelium. observed a induction activity when were grown as monolayer chronic...
Abstract Background α/β-hydrolase domain-containing protein 5 (ABHD5) plays an important role in the triacylglycerols (TAG) hydrolysis. Indeed, ABHD5 is co-activator of adipose triglyceride lipase (ATGL), that catalyses initial step TAG Mutations gene are associated with onset Chanarin-Dorfman syndrome (CDS), a rare autosomal recessive lipid storage disorder, characterized by non-bullous congenital ichthyosiform erythroderma (NCIE), hepatomegaly and liver steatosis. Case presentation We...
Estrogen receptor (ER) β acts as a tumor suppressor in malignant mesotheliomas. Here we explored the anti-proliferative and anti-tumorigenic efficacy of selective ERβ agonist, KB9520, human mesothelioma cell lines vitro mouse model vivo. KB9520 showed significant effect positive pleural cells vitro. Selective activation with sensitized to treatment cisplatin, resulting enhanced growth inhibition increased apoptosis. Furthermore, CD1 nude mice was significantly inhibited when added on top...