A5043320019- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Cannabis and Cannabinoid Research
- Air Quality and Health Impacts
- Parasitic Infections and Diagnostics
- Viral gastroenteritis research and epidemiology
- Pharmacology and Obesity Treatment
- Atmospheric chemistry and aerosols
- interferon and immune responses
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Alcohol Consumption and Health Effects
- Peroxisome Proliferator-Activated Receptors
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- RNA regulation and disease
- Amoebic Infections and Treatments
- Energy, Environment, Economic Growth
- COVID-19 impact on air quality
- SARS-CoV-2 detection and testing
- Pharmacological Receptor Mechanisms and Effects
- Toxoplasma gondii Research Studies
- Autonomous Vehicle Technology and Safety
- Health, Environment, Cognitive Aging
- Sperm and Testicular Function
National Institute for Parasitic Diseases
2024-2025
Sun Yat-sen University
2021-2025
Sun Yat-sen University Cancer Center
2021-2025
Qinghai University
2025
Southwest Jiaotong University
2023-2024
Guiyang Medical University
2024
China University of Mining and Technology
2023
The First People's Hospital of Guiyang
2023
Hangzhou Normal University
2023
Hefei University of Technology
2021
Analyses of viral protein-protein interactions are an important step to understand protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid system screen the genome-wide intraviral SARS coronavirus (SARS-CoV) therefrom revealed number novel which could be partly confirmed by in vitro biochemical assays. Three pairs identified were detected both directions: non-structural (nsp) 10 nsp14, nsp10 nsp16, nsp7 nsp8. The between multifunctional...
Analysis of viral protein-protein interactions is an essential step to uncover the protein functions and molecular mechanism for assembly a complex. We employed mammalian two-hybrid system screen all proteins SARS-CoV-2 interactions.Our study detected 48 interactions, 14 which were firstly reported here. Unlike Nsp1 SARS-CoV, has most interacting partners among likely as hub proteins. Five self-interactions confirmed, five Nsp1/Nsp3.1, Nsp3.1/N, Nsp3.2/Nsp12, Nsp10/Nsp14, Nsp10/Nsp16,...
Background Fatty-acid-binding proteins (FABPs) are intracellular carriers for endocannabinoids, N-acylethanolamines, and related lipids. Previous work indicates that systemically administered FABP5 inhibitors produce analgesia in models of inflammatory pain. It is currently not known whether FABP exert their effects through peripheral or central mechanisms. Here, we examined distribution dorsal root ganglia spinal cord the analgesic peripherally centrally inhibitors. Results...
Understanding the process of replication and transcription SARS-CoV-2 is essential for antiviral strategy development. The replicase polyprotein indispensable viral replication. However, whether all nsps derived from are not fully understood. In this study, we utilized replicon as system to investigate role each nsp in We found that except nsp16, deletions drastically impair replicon, nsp14 could recover deficiency caused by its deletion replicon. Due unsuccessful expressions nsp1, nsp3,...
Using panel data on 30 provinces in China from 2005 to 2012, this paper conducts an empirical test the threshold effect of relationship between financial development and carbon emission intensity perspectives scale efficiency. The results show that at a low level per capita GDP, expansion enhancement efficiency will increase intensity. When GDP is greater than value (RMB 37,410), also intensity, but potency be weaker. At same time, improvement help reduce Most with 37,410) are located...
Liver fatty acid-binding protein (FABP1, L-FABP) has high affinity for and enhances uptake of arachidonic acid (ARA, C20:4, n-6) which, when esterified to phospholipids, is the requisite precursor synthesis endocannabinoids (EC) such as arachidonoylethanolamide (AEA) 2-arachidonoylglycerol (2-AG). The brain derives most its ARA from plasma, taking up transporting it intracellularly via cytosolic proteins (FABPs 3,5, 7) localized within brain. In contrast, much more prevalent FABP1 not...
Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. infection due to close contact with pet dogs identification zoonotic genotypes, 589 fresh fecal specimens were collected from Yunnan Province, China determine occurrence , characterize dog-derived isolates, assess their potential at genotype level. was identified genotyped by PCR sequencing internal transcribed spacer (ITS)...
Abstract Background Cryptosporidium spp. and Enterocytozoon bieneusi are zoonotic pathogens with global distribution, infecting humans various livestock. For yaks, Tibetan sheep horses, the traditional grazing models still hold a main position. After these animals become infected, it not only hinders development of animal husbandry but also increases risk E. transmission between livestock herdsmen. Methods In present study, total 750 fecal samples were collected from horses in Shiqu County,...
Rationale: Myogenesis is a strictly regulated process driven by signaling pathways activating muscle-specific gene expression. During myogenesis, muscle stem cells exhibit DNA damage response (DDR) features, which are essential for myoblast differentiation and skeletal regeneration. However, the specific roles of DDR-associated proteins in these processes not yet fully understood. Methods: Gene knockdown knockout were used cell animal models to study RNF138's function Multi-omics profiling,...
<title>Abstract</title> <bold>Background:</bold> Faba bean (<italic>Vicia faba</italic> L.) is a crucial cool-season legume crop, which highly valued for its high protein content and key role in crop rotation systems. Considering the increasing threat of soil salinity alkalinity globally, it critical to screen germplasm resources with salt alkali tolerance faba identify underlying genes. <bold>Results:</bold> In this study, 12 morphological physiological traits under compound saline-alkali...
Fatty acid amide hydrolase 2 (FAAH2) is a that mediates the degradation of endocannabinoids in man. Alterations endocannabinoid system are associated with wide variety neurologic and psychiatric conditions, but phenotype biochemical characterization patients genetic defects FAAH2 activity have not previously been described. We report male autistic features an onset before age years who subsequently developed additional including anxiety, pseudoseizures, ataxia, supranuclear gaze palsy,...
We used a bovine brain cDNA library to perform yeast two‐hybrid assay with mature PrP C as bait. The screening result showed that αB‐crystalline interacted . interaction was further evaluated both in vivo and vitro different methods, such immunofluorescent colocalization, native polyacrylamide‐gel electrophoresis, IAsys biosensor assays. results suggested may have the ability refold denatured prion proteins, provided first evidence is directly associated protein.
Although liver fatty acid binding protein (FABP1, L-FABP) is not detectable in the brain, Fabp1 gene ablation (LKO) markedly increases endocannabinoids (EC) brains of male mice. Since brain EC system females differs significantly from that males, it was important to determine if LKO differently impacted system. did alter levels arachidonic (ARA)-containing EC, i.e. arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), but decreased non-ARA-containing N-acylethanolamides (OEA,...
3CLpro is a key proteinase for SARS-CoV-2 replication and serves as an important target antiviral drug development. However, how its activity regulated intracellularly still obscure. In this study, we developed protease reporter system to examine the impact of various factors, including nutrient supplements, ions, pHs, or oxidative stress inducers, on activity. We found that could increase overall 3CLpro. Not altering expression, decreased solubility in lysis buffer containing 1%...
Abstract The protein activator of kinase R (PKR) (PACT) has been shown to play a crucial role in stimulating the host antiviral response through activation PKR, retinoic acid‐inducible gene I, and melanoma differentiation‐associated 5. Whether PACT can inhibit viral replication independent known mechanisms is still unrevealed. In this study, we show that, like many viruses, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) hijacks GSK‐3β facilitate its replication. GSK‐3β‐induced...