A5047802437- Sinusitis and nasal conditions
- Head and Neck Surgical Oncology
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Nasal Surgery and Airway Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Tracheal and airway disorders
- Obstructive Sleep Apnea Research
- IL-33, ST2, and ILC Pathways
- Infectious Diseases and Mycology
- Olfactory and Sensory Function Studies
- Oral and Maxillofacial Pathology
- Transgenic Plants and Applications
- Immune Cell Function and Interaction
- Vascular Malformations and Hemangiomas
- Food Allergy and Anaphylaxis Research
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Pediatric health and respiratory diseases
- Respiratory and Cough-Related Research
- Head and Neck Cancer Studies
- Pleural and Pulmonary Diseases
- Energy Harvesting in Wireless Networks
- Long-Term Effects of COVID-19
Dokkyo Medical University
2014-2025
Jikei University School of Medicine
2014-2024
Stanford University
2017-2023
VA Palo Alto Health Care System
2023
Université Laval
2022
Institute for Biomedical and Pharmaceutical Research
2022
University of Bonn
2022
The University of Texas MD Anderson Cancer Center
2022
UCLouvain
2022
RIKEN Center for Integrative Medical Sciences
2015-2019
Abstract The coronavirus SARS-CoV-2 is the causative agent of ongoing severe acute respiratory disease pandemic COVID-19. Tissue and cellular tropism one key to understanding pathogenesis SARS-CoV-2. We investigate expression subcellular localization receptor, angiotensin-converting enzyme 2 (ACE2), within upper (nasal) lower (pulmonary) tracts human donors using a diverse panel banked tissues. Here, we report our discovery that ACE2 receptor protein robustly localizes motile cilia airway...
How SARS-CoV-2 penetrates the airway barrier of mucus and periciliary mucins to infect nasal epithelium remains unclear. Using primary epithelial organoid cultures, we found that virus attaches motile cilia via ACE2 receptor. traverses layer, using as tracks access cell body. Depleting blocks infection for other respiratory viruses. progeny attach microvilli 24 h post-infection trigger formation apically extended highly branched organize viral egress from back into supporting a model...
Endoscopic medial maxillectomy (EMM) is a safe and effective procedure for treatment of inverted papilloma (IP) originating from the maxillary sinus. However, EMM usually removes inferior turbinate nasolacrimal duct. The has critical function in conditioning nasal airflow, resection ducts risk epiphora. We developed newly derived surgical technique, endoscopic modified (EMMM), which enables preservation duct.A retrospective case series six patients with IP nine mucoceles sinus after...
Understanding the mechanisms of HIV tissue persistence necessitates ability to visualize microenvironments where infected cells reside; however, technological barriers limit our dissect cellular components these reservoirs. Here, we developed protein and nucleic acid in situ imaging (PANINI) simultaneously quantify DNA, RNA, levels within compartments. By coupling PANINI with multiplexed ion beam (MIBI), measured over 30 parameters across archival lymphoid tissues from healthy or simian...
Interleukin (IL)-33, which is a member of the IL-1 family cytokines, now recognized as an important contributor to Th2-type immune responses. We examined whether levels IL-33 in sera and nasal secretions are upregulated allergic rhinitis (AR) patients, we tested for correlations between level parameters atopy symptom score.The study included 24 Japanese cedar pollinosis patients (12 male 12 female with mean age 47.7 years) history moderate-to-severe AR, 14 house-dust-mite-sensitized AR (9 5...
The pathophysiology of chronic rhinosinusitis (CRS) is not fully understood. In Europe and the United States, major subsets CRS classification are based on presence or absence polyps. Although nasal polyps (NPs) a critical factor, many other factors also contribute to pathogenesis CRS. aim this study was investigate diverse phenotypes using cluster analysis.This multicenter examining clinical data from patients treated at five hospitals. design retrospective analysis prospectively collected...
Sinus fungus ball is defined as noninvasive chronic fungal rhinosinusitis occurring in immunocompetent patients with regional characteristics. The clinical and imaging characteristics of paranasal sinus were retrospectively investigated 104 Japanese patients. All underwent endoscopic surgery. Preoperative computed tomography (CT), magnetic resonance (MR) imaging, age, sex, chief complaint, causative fungus, outcome analyzed. Patients aged from 25 to 79 years (mean 58.8 years). Female...
Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is known to have 2 phenotypes in East Asia. Eosinophilic CRSwNP (ECRSwNP), defined as tissue eosinophilia and easily recurrent, distinguished from other non‐eosinophilic (NECRSwNP) types. However, the pathogenesis of each remains unclear. Methods Nasal polyp tissues ECRS (ECRSwNP) NECRS patients were obtained, their comprehensive gene expression profiles investigated by microarray analysis. Bioinformatics approaches (eg, Ingenuity...
Patients undergoing endoscopic sinus surgery (ESS) are at risk of complications because the close proximity sinuses to orbit and anterior skull base. The aim this study was evaluate ESS identify patient characteristics that were factors for complications.We conducted a prospective 706 patients who underwent chronic rhinosinusitis. completed preoperative examinations included computed tomography, observation nasal polyps, tests comorbidities including asthma vascular disease. Perioperative...
We investigated the expression and subcellular localization of SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within upper (nasal) lower (pulmonary) respiratory tracts healthy human donors. detected ACE2 protein cilia organelle ciliated airway epithelial cells, which likely represents initial or early site viral entry during transmission. further determined whether in cells was influenced by patient demographics, clinical characteristics, co-morbidities, medication use, found...
The respiratory disease COVID-19 is caused by the coronavirus SARS-CoV-2. Here we report discovery of ethacridine as a potent drug against SARS-CoV-2 (EC50 ~ 0.08 μM). Ethacridine was identified via high-throughput screening an FDA-approved library in living cells using fluorescence assay. Plaque assays, RT-PCR and immunofluorescence imaging at various stages viral infection demonstrate that main mode action through inactivation particles, preventing their binding to host cells....
Eosinophilic otitis media (EOM) is a refractory condition associated with eosinophilic chronic rhinosinusitis and bronchial asthma. EOM characterized by type-2 inflammation to various treatments. We investigated the efficacy of dupilumab, interleukin-4 receptor alpha antagonist, for patients complicated (ECRS).Between April 2017 2022, we treated 124 dupilumab CRS or Of these, 14 had concurrently, 10 them who been >6 months were included in our study. retrospectively evaluated amount systemic...
Reverse transcription-quantitative polymerase chain reaction is a valuable and reliable method for gene quantification. Target expression usually quantified by normalization using reference genes (RGs), accurate critical producing data. However, stable RGs in nasal polyps sinonasal tissues from patients with chronic rhinosinusitis (CRS) have not been well investigated. Here, we used two-stage study design to identify RGs. We assessed the stability of 15 commonly candidate five...
Background Eosinophilic chronic rhinosinusitis (eCRS) is a type 2 inflammatory disease that frequently recurs after surgery. In recent years, dupilumab has been available for the treatment of refractory since 2020 in Japan. Although there are some reports its usefulness, not enough clinical efficacy longer than 1 year, especially olfactory recovery. Methods Twenty patients with eCRS who had recurrence surgery and receiving were enrolled retrospectively. The nasal polyp score (NPS), computed...
ABSTRACT Background Allergic fungal rhinosinusitis (AFRS) is a subtype of chronic driven by Types 1 and 3 allergies to fungi. In Japan, it relatively rare characterized prominent eosinophilic infiltration the sinonasal mucosa, together with mucin containing scattered fungi in sinus cavity. Eosinophilic (eCRS) involves similar shares some clinical features AFRS. However, differences between eCRS AFRS remain be fully elucidated. The aim this study was clarify phenotypes Methods This...