A5074998391- Glioma Diagnosis and Treatment
- Analytical Chemistry and Chromatography
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracranial Aneurysms: Treatment and Complications
- Radiomics and Machine Learning in Medical Imaging
- Advanced Fluorescence Microscopy Techniques
- Chemical Thermodynamics and Molecular Structure
- Vascular Malformations Diagnosis and Treatment
- Microfluidic and Capillary Electrophoresis Applications
- Crystallization and Solubility Studies
- Photoacoustic and Ultrasonic Imaging
- Luminescence Properties of Advanced Materials
- Advanced Chemical Sensor Technologies
- Brain Metastases and Treatment
- Anesthesia and Neurotoxicity Research
- Fermentation and Sensory Analysis
- Inflammatory Biomarkers in Disease Prognosis
- Intensive Care Unit Cognitive Disorders
- Prostate Cancer Treatment and Research
- Renal cell carcinoma treatment
- Chromatography in Natural Products
- Cancer, Lipids, and Metabolism
- Glass properties and applications
- Medical Imaging Techniques and Applications
- Cell Image Analysis Techniques
Institute of Molecular and Clinical Ophthalmology Basel
2025
University of Basel
2025
University of Bonn
2014-2024
University Hospital Schleswig-Holstein
2006-2024
University of Lübeck
2007-2024
Universitäres Kinderwunschzentrum Lübeck
2020
Leibniz Institute of Photonic Technology
2007-2008
Heidelberg University
2007
University Hospital Heidelberg
2007
University of Göttingen
2007
X-ray attenuation measurements are widely used in medical and industrial applications. The usual results one- to three-dimensional representations of the coefficient μ(r). In this paper, we present ρZ projection algorithm for obtaining density ρ(r) atomic number Z(r) with an energy-resolving x-ray method. As input data uses at least two μ1,μ2,… different spectral weightings source spectrum S(E) and/or detector sensitivity D(E). Analytically, ρ is a function μ1−cμ2, c=const, Z μ1/μ2. full...
Stargardt disease is a currently untreatable, inherited neurodegenerative that leads to macular degeneration and blindness due loss-of-function mutations in the ABCA4 gene. We have designed dual adeno-associated viral vector encoding split-intein adenine base editor correct most common mutation (c.5882G>A, p.Gly1961Glu). optimized editing human models, including retinal organoids, induced pluripotent stem cell-derived pigment epithelial (RPE) cells, as well adult explants RPE/choroid vitro....
Abstract Introduction Detection of residual tumor during resection glial brain tumors remains a challenge because low inherent contrast adjacent edematous brain, the surrounding infiltration zone, and solid tumor. Therefore, new technologies that may facilitate an intraoperative analysis tissue at edge are great interest to neurosurgeons. Materials Methods For ex vivo imaging gliomas in mouse model human biopsy specimens nervous system we have used time‐domain Sirius 713 Tomograph with...
Thrombocytosis, which is defined as a platelet count greater than 400 platelets/nl, has been found to be an independent predictor of shorter survival in various tumors. Release growth factors from tumors proposed increase counts. Preoperative counts and other clinical hematological parameters were reviewed the records 153 patients diagnosed between 1999 2004 with histologically confirmed glioblastoma order evaluate prognostic significance preoperative thrombocytosis these patients. The...
Ischemic complications account for significant patient morbidity following aneurysmal subarachnoid hemorrhage (aSAH). The Prevention and Treatment of Vasospasm with Clazosentan (REACT) study was designed to assess the safety efficacy clazosentan, an endothelin receptor antagonist, in preventing clinical deterioration due delayed cerebral ischemia (DCI) patients aSAH.
Intraoperative detection of residual tumor tissue in glioma surgery remains an important challenge because the extent removal is related to prognosis disease. Multiphoton excited fluorescence tomography living tissues provides high-resolution structural and photochemical imaging at a subcellular level. In this conceptual study, we have used multiphoton microscopy lifetime (4D microscopy) image cultured cell lines, solid tumor, invasive cells experimental mouse model human biopsy specimens.A...
Abstract BACKGROUND: Because of their rarity, no prospective studies have been performed regarding gangliogliomas. The optimal treatment regimen is unclear. In this study, the authors compared 4 therapies for local control (LC) and overall survival (OS) in patients with ganglioglioma. METHODS: 402 ganglioglioma, outcomes were who underwent gross total resection alone (GTR) (n = 188), GTR plus radiotherapy (GTR + RT) 21), subtotal (STR) 113), STR RT (STR 80). Age, sex, tumor site, histologic...
Multiphoton excitation fluorescent microscopy is a laser-based technology that allows subcellular resolution of native tissues in situ. We have recently applied this to the structural and photochemical imaging cultured glioma cells experimental gliomas ex vivo. demonstrated high microanatomical definition tumor, invasion zone, normal adjacent brain can be obtained down single-cell unprocessed tissue blocks. In study, we used multiphoton four-dimensional generate fluorescence lifetime maps...
Negative temperature gradients along the gas chromatographic separation column can maximize capabilities for chromatography by peak focusing and also lead to lower elution temperatures. Unfortunately, so far a smooth thermal gradient over several meters long could only be realized costly complicated manual setups. Here we describe simple, yet flexible method generation of negative using standard easily exchangeable columns. The measurements made with first prototype reveal promising new...
The Deans' switch is an effluent switching device based on controlling flows of carrier gas instead mechanical valves in the analytical flow path. This technique offers high inertness and a wear-free operation. Recently new monolithic microfluidic devices have become available. In these whole system integrated into small metal with low thermal mass leak-tight connections. contrast to valve-based system, flow-controlled more difficult calculate. Usually used one inlet two outlets, by means...
Hyperfast GC/MS below 60 s of measurement time has been used for the explosives. The new flow-field thermal gradient GC (FF-TG-GC) utilizes a modified transport process explosives at lowered temperatures. In combination with focusing effect gradient, high-resolution chromatograms are obtained even in very short intervals. reduction elution temperature by applying along chromatographic column is demonstrated simulation migration analytes through column. shows an interesting difference between...
Glioblastoma is more common in men than women. The aim of this analysis was to investigate sex-specific differences with a particular focus on their impact survival including overall (OS) and progression-free (PFS). This retrospective study analyzed 209 GBM patients (91 females, 118 males) treated according the Stupp regimen. Data patient demographics, O6-methylguanine-DNA methyltransferase (MGMT) methylation status, treatment details [radiotherapy (RT) doses temozolomide (TMZ) cycles],...
The clinical usefulness of 5-ALA guided detection tumor tissue has been demonstrated for a number malignancies. However, current techniques intraoperative protoporphyrin IX fluorescence in situ do not offer subcellular resolution. Therefore, discrimination non-specific induced remains difficult.In this study we have used an orthotopic glioma model to analyze PpIX and normal brain by multiphoton excitation microscopy after intraperitoneal administration 5-ALA. A DermaInspect vivo imaging...
Recent studies have highlighted the importance of complete resection a brain tumour but task often remains challenge for neurosurgeon. New technologies which add objective information beyond visualisation provided by traditional operating microscope are required. In this study, we analysed cellular density tumour/brain interface using three dimensional multi-photon microscopy intensity-images experimental gliomas and human brain-tumour biopsy samples. The nuclei was determined in specimens...
This work presents an open source database with suitable retention parameters for prediction and simulation of GC separations gives a short introduction to three common models. Useful computer simulations play important role save resources time in method development GC. Thermodynamic the ABC model K-centric are determined by isothermal measurements. standardized procedure measurements calculations, presented this work, have useful benefit all chromatographers, analytical chemists, developers...
Optical coherence tomography (OCT) is a non-invasive imaging technique with micrometer resolution. It allows non-contact / analysis of central nervous system tissues penetration depth 1-3,5 mm reaching spatial resolution approximately 4-15 μm. We have adapted spectral-domain OCT (SD-OCT) and time-domain (TD-OCT) for intraoperative detection residual tumor during brain surgery. Human tissue areas the resection cavity were analyzed gliomas using this new technology. The site was...
Abstract Background Recently, antibodies against the alpha isoform of glial-fibrillary-acidic-protein (GFAPα) were identified in a small series patients with encephalomyelitis. Coexisting autoantibodies (NMDA receptor, GAD65 antibodies) have been described few these patients. We describe patient rapidly progressive encephalomyeloradiculitis and combination anti-ITPR1, anti-GFAP anti-MOG antibodies. Case presentation literature review A 44-year old caucasian woman flu-like prodrome presented...