Conventional autologous skin grafts are associated with significant donor-site morbidity. This study was conducted to determine feasibility, safety, and efficacy of a new strategy for grafting based on harvesting small columns full-thickness minimal morbidity.The swine model used this study. Hundreds tissue (~700 µm diameter) were harvested using custom-made device, then applied directly excisional wounds. Healing in donor graft sites evaluated over 3 months by digital photographic...

Full-thickness skin loss is a challenging problem due to limited reconstructive options, demanding 75 million surgical procedures annually in the United States. Autologous grafting gold standard treatment, but results donor-site morbidity and poor aesthetics. Numerous substitutes are available on market date, however, none truly functions as full-thickness lack of vascular network. The creation an autologous analogue with pedicle would result paradigm shift management wounds reconstruction...

We present evidence-based design principles for three different UV-C based decontamination systems N95 filtering facepiece respirators (FFRs) within the context of SARS-CoV-2 outbreak 2019-2020. The approaches used here were created with consideration needs low- and middle-income countries (LMICs) other under-resourced facilities. As such, a particular emphasis is placed on providing cost-effective solutions that can be implemented in short order using generally available components...

AIM:To investigate the impact of arachidonic acid (AA) and docosahexaenoic (DHA) their combination on colon cancer cell growth. METHODS:The LS-174T line was used to study role prostaglandin precursor AA omega-3 polyunsaturated fatty DHA growth.Cell viability assessed in XTT assays.For analysis cycle death, flow cytometry DAPI staining were applied.Expression cyclooxygenase-2 (COX-2), p21 bcl-2 cells incubated with or examined by real-time RT-PCR.Prostaglandin E2 (PGE2) generation presence...

Abstract UV‐C emitting nanoscale scintillators can be used to sensitize cancer cells selectively against X‐rays during radiation therapy, due the lethal DNA lesions caused by photons. Unfortunately, particles (NPs) show decreased emission intensity. In this paper, influence of different Nd 3+ concentrations on micro‐ and LuPO 4 :Pr is investigated upon X‐ray irradiation vacuum UV excitation (160 nm). Co‐doped results in increased independent source energy transfer from Pr . The highest...

Background and Objectives Laser hair removal is the most common laser therapy third commonly performed procedure with more than one million treatments in United States 2016. This retrospective study was conducted to assess long‐term efficacy safety of 755 nm for removal. Study Design/Materials Methods Nearly, 3,606 were long‐pulsed wavelength equipped an epidermal cooling device between 1997 2005 followed till 2013. Standardized assessments by two treating physicians patients at follow‐up...

Cryolipolysis is a noninvasive method for removal of subcutaneous fat body contouring. Conventional cryolipolysis with topical cooling requires extracting heat from by conduction across the skin, thus limiting amount and location removed. The authors hypothesized that local injection physiological ice slurry directly into target adipose tissue would lead to more efficient effective cryolipolysis.

Purpose: Individuals suffering from Fanconi Anemia (FA) exhibit a pronounced hypersensitivity to agents that cause DNA inter-strand crosslinks and frequently also ionising radiation. However, fibroblast lines derived FA patients generally show little or no radiosensitivity in vitro. Here, we sought elucidate the role of central protein D2 (FANCD2) determining cellular radioresistance.

Purpose Radiation therapy for cancer is limited by damage to surrounding normal tissues, and failure completely eradicate a tumor. This study investigated novel radiosensitizer, composed of lutetium phosphate nanoparticles doped with 1% praseodymium 1.5% neodymium cations (LuPO4:Pr3+,Nd3+). During X-ray exposure, the particles emit UVC photons (200–280 nm), resulting in increased tumor cell death, oxygen-independent UVC-induced damage.Methods Materials Specially designed LuPO4:Pr3+,Nd3+...

Genetic or epigenetic inactivation of the pathway formed by Fanconi anemia (FA) and BRCA1 proteins occurs in several cancer types, making affected tumors potentially hypersensitive to DNA cross‐linkers other chemotherapeutic agents. It has been proposed that inability FA/BRCA‐defective cells form subnuclear foci effector proteins, such as FANCD2, can be used a biomarker aid individualization chemotherapy. We show FANCD2 not only renders sensitive cross‐links, but also oxidative stress,...

Radiation injury to the skin is a major source of dysfunction, disfigurement, and complications for thousands patients undergoing adjunctive treatment internal cancers. Despite great potential affecting quality life, radiation has received little attention from dermatologists primarily being managed by oncologists. During our volunteer work in Vietnam, we encountered numerous children with significant scarring depigmentation outdated use radioactive phosphorus P32 hemangiomas. This dangerous...

Meralgia paresthetica (MP) is a rare lateral femoral cutaneous nerve-(LFCN)-mononeuropathy. Treatment for this disorder includes conservative and operative approaches; the latter considered if therapy fails. The most commonly used surgical approaches are decompression/neurolysis avulsion/neurectomy. However, there no definitive guidelines on optimal approach to be used. purpose of study was evaluate outcome decompression LFCN treatment persistent MP with preservation sensation along...

Radiation-resistant hypoxic tumor areas continue to present a major limitation for successful treatment. To overcome this radiation resistance, an oxygen-independent treatment is proposed using UVC-emitting LuPO4:Pr3+ nanoparticles (NPs) and X rays. The uptake of the NPs as well their effect on cell proliferation was investigated A549 lung cancer cells by inverted time-lapse microscopy transmission electron microscopy. Furthermore, cytotoxicity combined rays assessed under normoxic...

Background and Objective Skin changes are among the most visible signs of aging. Fractional ablative lasers improve skin quality by making small wounds that heal rapidly without scarring. While they texture discoloration, there is minimal effect on laxity. This study was performed to assess shrinkage removing multiple full‐thickness columns with coring needles combined wound closure. Materials Methods In 5 swine 116 squares (3 cm 2 ) were demarcated for treatment control sites. sites 10%...

Objective Lidocaine acts as a local anesthetic by blocking transmembrane sodium channel permeability, but also induces the synthesis of heat shock proteins and sensitizes cells to hyperthermia. A previous study reported two cases deep focal skin ulceration at points corresponding depot lidocaine injection sites after treatment with non‐ablative fractional resurfacing it was hypothesized that had focally sensitized keratinocytes thermal damage laser treatment. The objective this investigate...

Epidermal suction blister grafts are an effective treatment for chronic wounds or vitiligo, but this is time consuming and limited to small areas.To compare two novel strategies create fractional epidermal grafts.Epidermal blisters were raised from fresh human skin ex vivo at 38-40 °C, with of 380-510 mmHg. In Strategy 1, a 1-cm was micromeshed into approximately 500 pieces, transferred elastic adhesive dressing, then pneumatically expanded nine times the original area. 2, 25-cm(2) array 100...

To compare the induction and repair of radiation-induced base damage in human rodent cell lines.Experiments were performed with two (normal fibroblasts HSF1 tumour HeLa cells) (mouse L929 hamster CHO-K1) lines. Base was determined alkaline comet assay combined enzyme formamidopyrimidine-glycosylase (Fpg). Proteins detected by Western blot.The Fpg-sensitive sites measured lines for doses up to 8 or 5 Gy, respectively. Comets analysed terms tail moments, which transformed into Gy-equivalents....

As an alternative method to conventional split-thickness skin grafts (STSGs), we recently proposed fractional grafting (FSG), which consists in harvesting hundreds of microscopic tissue columns (MSTCs) place them directly into the wound (Tam et al., 2013, “Fractional Skin Harvesting: Autologous Graft Without Donor Site Morbidity,” Plast. Reconstructive Surgery–Global Open, 1(6)). This paper (i) introduces concept and operational principles a simple but robust (FSH) device (ii) presents...

Ablative fractional laser treatment uses thousands of very small beam wounds to damage a fraction the skin, which stimulates tissue remodeling. Each open micro-wound heals without scarring, but amount skin tightening achieved is limited. This animal study was performed test hypothesis that immediate temporary closure could increase after ablative treatment.Four adult swine were used for study; 98 square sites (3 × 3 cm) tattooed on abdomen and flanks each pig. An Erbium:YAG (Sciton Profile,...