A5012717159- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Neuroscience and Neuropharmacology Research
- Gene Regulatory Network Analysis
- Nanoplatforms for cancer theranostics
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Radiomics and Machine Learning in Medical Imaging
- RNA Research and Splicing
- Acute Lymphoblastic Leukemia research
- Pharmaceutical studies and practices
- Abdominal Surgery and Complications
- Appendicitis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- Immune Response and Inflammation
- Erythropoietin and Anemia Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Infective Endocarditis Diagnosis and Management
- NF-κB Signaling Pathways
- Radiopharmaceutical Chemistry and Applications
ETH Zurich
2014-2024
The Geneva Association
2024
Janssen (Germany)
2023
Amsterdam Neuroscience
2015
University Medical Center Hamburg-Eppendorf
2009-2014
Universität Hamburg
2009-2014
University of Pittsburgh
2009
Whitehead Institute for Biomedical Research
2005-2006
The NF-kappaB family of transcription factors plays a critical role in numerous cellular processes, particularly the immune response. Our understanding how different subunits act coordinately to regulate gene expression is based on limited set genes. We used genome-scale location analysis identify targets all five proteins before and after stimulation monocytic cells with bacterial lipopolysaccharide (LPS). In unstimulated cells, p50 p52 bound large number promoters that were also occupied...
Abstract Motivation: Genome-wide chromatin-immunoprecipitation (ChIP-chip) detects binding of transcriptional regulators to DNA in vivo at low resolution. Motif discovery algorithms can be used discover sequence patterns the bound regions that may recognized by immunoprecipitated protein. However, discovered motifs often do not agree with specificity protein, when it is known. Results: We present a powerful approach analyzing ChIP-chip data, called THEME, tests hypotheses concerning...
Significance Synthetic biology and genetic engineering would greatly benefit from engineered elements that are orthogonal to the host in which they operate. Two-component signaling pathways prevalent signal processing modality prokaryotes is also found low eukaryotes plants but absent vertebrate cells. Here we investigate whether of prokaryotic two-component operational mammalian We find core biochemical processes maintained, whereas capacity sense chemical ligands diminished or obscured....
Synaptic plasticity requires remodeling of the actin cytoskeleton. Although two isoforms, β- and γ-actin, are expressed in dendritic spines, specific contribution γ-actin expression synaptic is unknown. We show that levels regulated by E3 ubiquitin ligase TRIM3. TRIM3 protein Actg1 transcript colocalized messenger ribonucleoprotein granules responsible for targeting RNAs. polyubiquitylates most likely cotranslationally at sites. Trim3−/− mice consequently have increased hippocampal synapses,...
Abstract Introduction The prognoses of critically ill patients with a requirement for emergency laparotomy and severe respiratory and/or hemodynamic instability precluding transport to the operating room (OR) are often fatal without surgery. Attempting surgery at bedside might equally result in an adverse outcome. However, risk factors predictors that could support clinical decision making have not been identified so far. This study describes characteristics, indicative pathophysiology...
Abstract Constructing gene circuits that satisfy quantitative performance criteria has been a long‐standing challenge in synthetic biology. Here, we show strategy for optimizing complex three‐gene circuit, novel proportional mi RNA biosensor, using predictive modeling to initiate search the phase space of sensor genetic composition. We generate library diverse building blocks order access favorable parameter combinations and uncover specific compositions with greatly improved dynamic range....
BACKGROUND: Preoperative autologous blood donation (PABD) and intraoperative salvage (IBS) represent established conservation measures. However, data comparing PABD to IBS are very sparse. STUDY DESIGN AND METHODS: We analyzed from 1103 patients undergoing subsequent major orthopedic surgery in one center. then used a validated model compare IBS. calculated maximal allowable losses (MABLs) for both PABD. also identified criteria efficacious use of either or Our calculations were based on...
Gene therapy that regulates an antitumor gene via a multi-input biomolecular logic module treats multifocal tumors in mouse model of liver cancer.
Cell classifier gene circuits that integrate multiple molecular inputs to restrict the expression of therapeutic outputs cancer cells have potential result in efficacious and safe therapies. Preclinical translation hitherto developments requires creating conditions where animal model, delivery platform, vivo levels inputs, efficacy output, all come together enable detailed evaluation fully assembled circuits. Here we show an integrated workflow addresses these issues builds framework for...
Sphingosine-1-phosphate (S1P) is a bloodborne lipid that regulates vascular tone and endothelial permeability. S1P concentrations are reduced in critically ill patients. As hematopoietic cells produce S1P, this study intends to investigate blood products during storage patient plasma after transfusion.S1P were measured 83 red cell (RBC) units 73 platelet concentrates (PCs) before storage. In addition, 26 patients who received one or two RBC recruited measure levels three times within 24...
Abstract The European Medicines Agency approved ustekinumab for psoriasis treatment in adolescents (12–17 years) 2015 (CADMUS trial), and children (6–11 2020. Interim results are reported from a post-authorization study (NCT03218488) evaluating the long-term safety profile of ustekinumab. This ongoing, prospective, observational, multicentre enrolled patients (6–17 diagnosed with moderate-to-severe plaque initiating Clinical data all adverse events (AEs) through approximately 54 months (data...
Abstract Aberrant activation of epidermal growth factor receptor (EGFR) signaling pathways is associated with tumor and progression. EGFR overexpression occurs in various types solid cancers correlates poor outcome. Unfortunately, the clinical benefit from EGFR-targeted therapies usually short-lived, due to development resistance. TargImmune has developed first-in-class Targeted Apoptotic Immunomodulators (TAIMs), which harness body’s anti-viral responses treat tumors. Our novel nanoparticle...