A5013727947- Phytochemicals and Antioxidant Activities
- Microbial Natural Products and Biosynthesis
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Marine Sponges and Natural Products
- Plant-Microbe Interactions and Immunity
- Phytochemical compounds biological activities
- Essential Oils and Antimicrobial Activity
- Marine Biology and Environmental Chemistry
- Plant Pathogenic Bacteria Studies
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Alkaloids: synthesis and pharmacology
- Natural product bioactivities and synthesis
- Coral and Marine Ecosystems Studies
- TGF-β signaling in diseases
- Environmental Toxicology and Ecotoxicology
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolism and Applications
- Natural Antidiabetic Agents Studies
- Chemical synthesis and alkaloids
- Microbial Inactivation Methods
- Phytochemistry and Bioactive Compounds
- Meat and Animal Product Quality
Gannan Normal University
2017-2025
Inner Mongolia Agricultural University
2025
Tongji Hospital
2025
Huazhong University of Science and Technology
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2022
Guangxi Normal University
2017-2019
Dalian Ocean University
2017-2018
Hong Kong University of Science and Technology
2013-2017
University of Hong Kong
2013-2017
South China Agricultural University
2014-2016
G protein-coupled receptors (GPCRs), which constitute the largest and structurally best conserved family of signaling molecules, are involved in virtually all physiological processes. Crystal structures available only for detergent-solubilized light receptor rhodopsin. In addition, this is GPCR presumed higher order oligomeric state native membranes has been demonstrated (Fotiadis, D., Liang, Y., Filipek, S., Saperstein, D. A., Engel, Palczewski, K. (2003) Nature 421, 127–128). Here, we have...
The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation even larger oligomeric complexes. Here, we have investigated the organization prototypical GPCR rhodopsin its native membrane by electron atomic force microscopy (AFM). Disc from mice were isolated observed AFM at room temperature. In all experimental conditions, forms structural dimers organized paracrystalline arrays. A semi-empirical molecular model for paracrystal is...
Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) is the major cultivar planted in China. Almost all peels produced that country are currently discarded, which not only harmful to environment but also a waste of resources. It therefore necessary carry out research explore utilization potential this resource.A 95% ethanol extract (EE) peel was prepared and fractionated into three subextracts: petroleum ether (PEE), ethyl acetate (EtOAcE), water (WE) by simple liquid / extractions....
The visual process is initiated by the photoisomerization of 11-<i>cis</i>-retinal to all-<i>trans-</i> retinal. For sustained vision 11-<i>cis</i>-chromophore must be regenerated from all-<i>trans</i>-retinal. This requires RPE65, a dominant retinal pigment epithelium protein. Disruption <i>RPE65</i> gene results in massive accumulation all-<i>trans</i>-retinyl esters epithelium, lack and therefore rhodopsin, ultimately blindness. We reported previously (Van Hooser, J. P., Aleman, T. S.,...
The present study investigates the chemical composition and antioxidant capacity of juice from Gannan navel orange, which is harvested at one- to two-week intervals during ripening period. total soluble solid (TSS), polyphenol content (TPC), flavonoid (TFC), sucrose hesperidin contents gradually increase with fruit, followed by slight declines late maturity stage. Contrary these observations, titratable acid (TA), vitamin C (Vc), limonin trend downward throughout However, fructose, glucose,...
The present study investigated the antibacterial mechanism, control efficiency, and nontarget toxicity of actinomycin X2 (Act-X2) against Xanthomonas citri subsp. (Xcc) for first time. Act-X2 almost completely inhibited proliferation Xcc in growth curve assay at a concentration 0.25 MIC (minimum inhibitory concentration, = 31.25 μg/mL). This effect was achieved by increasing production reactive oxygen species (ROS), blocking formation biofilms, obstructing synthesis intracellular proteins,...
Summary A gene bank of Azospirillum lipoferum Br17 constructed in the vector λGEM11 was screened with a Bradyrhizobium japonicum nifA probe. 7.3 kb EcoRI fragment carrying ‐like thereby isolated and subsequently used to screen brasilense Sp7 pUC18. Two fragments 5.6 3.6 covering ‐homology region were found. Mutants Nif ‐ phenotype obtained by site‐directed Tn5 mutagenesis subsequent recombination into A. genome. The mutations clustered two loci located at each extremity fragment. One these...
A new phthalide derivative, xylarphthalide (1), along with two known compounds (-)-5-carboxylmellein (2) and (-)-5-methylmellein (3), were isolated from the endophytic fungus Xylaria sp. GDG-102 cultured Chinese medicinal plant Sophora tonkinensis. Their structures identified by MS NMR experiments, absolute configuration of 1 was further confirmed single-crystal X-ray diffraction analysis. Compound 1–3 showed antibacterial activities against Bacillus megaterium, subtilis, Staphylococcus...
The antioxidant effect of 95% ethanol extract and its three subfractions, PE (petroleum ether), EtOAc (ethyl acetate), water extracts, from Gannanzao navel orange peel, were evaluated by ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (1,1-diphenyl-2-picryl-hydrazyl) FRAP (ferric reducing/antioxidant potential) methods for the first time. Furthermore, TPC (total polyphenol content), TFC flavonoid primary individual flavonoids four extracts analyzed compared. results...
Citrus canker, caused by Xanthomonas citri subsp. (Xcc), is a severe citrus disease. Currently, copper-containing pesticides are widely used to manage this disease, posing high risks the environment and human health. This study reports discovery of naturally occurring anti-Xcc compounds from deep-sea fungus, Aspergillus terreus SCSIO 41202, possible mode action. The ethyl acetate extract A. was subjected bioassay-guided isolation, resulting in eight (1–8) with minimum inhibitory...
The effects of five different drying methods, namely, freeze (FD), shade (SD), hot-air oven at 50 °C (OD50), 70 (OD70), and microwave (MD) on the bioactive phytochemicals antioxidant capacity navel orange peel were assessed comprehensively discussed in detail. Compared with other MD-treated contained lowest total phenolic content (TPC) flavonoid (TFC). subjected to OD70 treatment was superior TPC relative treatments highest TFC found peels treated FD. HPLC analysis identified thirteen...
Citrus canker and citrus blue mold are two severe diseases in plants, which mainly caused by Xanthomonas citri susp. (Xcc) Penicillium italicum, respectively. The currently widely used pesticides for these harmful to human health the environment. Therefore, searching novel antimicrobial agents, especially from natural resources, is getting increasing interest.In this study, crude extract of Streptomyces sp. GLL-9, an endophyte a navel orange tree, was found exhibit excellent effects against...
Chemical investigation on the South China Sea gorgonian coral Subergorgia suberosa has led to isolation of one new butenolide (5R)-5-(1-ethoxypropyl)-5-hydroxy-3,4-dimethylfuran-2(5H)-one (1) as a pair inseparable epimers, along with two known butenolides (S)-5-hydroxy-3,4-dimethyl-5-propylfuran-2(5H)-one (2) and (S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one (3). Their structures including absolute configurations were determined unambiguously by detailed analyses spectroscopic data...
Long-term fine particulate matter (PM2.5) exposure was associated with childhood obesity. However, the key PM2.5 components and whether effect may vary by obesity type, growth stage, sex, individual/family characteristics have yet been examined. In this study, we investigated 213,907 Chinese children adolescents aged 3-18 years in 2017-2019. Three-year average concentrations of five major were assigned to each participant's address. Multivariable mixed-effects model weighted quantile sum...
The present study reported the discovery of sesterdiaporate (SES), a structurally unique antifungal sesterterpene lactone from Diaporthe sp. HT-79. SES exhibited pronounced inhibitory effect on spore germination and mycelial growth Penicillium italicum, with median effective concentrations (EC50) 0.37 0.079 μg/mL, respectively. This was significantly more than that observed prochloraz (0.74 6.83 respectively). Treatment resulted in swelling both spores mycelia P. italicum as well an increase...
Agropyron michnoi is a perennial grass with rhizomes in the genus Agropyron. It has strong tolerance to drought and low temperature, it an established species sandy flat hilly slope lands, which constitute grassland. So, important forage dry grassland desert steppes. Rhizomes not only enable asexual reproducibility but also confer resilience stresses A. michnoi. However, during production utilization, been found that there are significant differences development of among individuals michnoi,...