A5064174082- Microbial Fuel Cells and Bioremediation
- 3D Printing in Biomedical Research
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Chemistry and Chemical Engineering
- biodegradable polymer synthesis and properties
- Molecular Junctions and Nanostructures
- Pluripotent Stem Cells Research
- Radical Photochemical Reactions
- Law, Rights, and Freedoms
- Healthcare Education and Workforce Issues
- CRISPR and Genetic Engineering
- CO2 Reduction Techniques and Catalysts
- Bacterial biofilms and quorum sensing
- RNA modifications and cancer
- Diabetic Foot Ulcer Assessment and Management
- American Constitutional Law and Politics
- Click Chemistry and Applications
- RNA Research and Splicing
- Microplastics and Plastic Pollution
- Advanced Polymer Synthesis and Characterization
University of California, San Francisco
2022-2025
The University of Texas at Austin
2018-2025
Chan Zuckerberg Initiative (United States)
2022-2024
University of California, Santa Barbara
2018
Abstract Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, neuromorphic computing. Despite their potential, developing programmable modular methods living systems to interface with OECTs has proven challenging. Here we describe hybrid containing the model electroactive bacterium Shewanella oneidensis that enable transduction of computations responses. Specifically,...
While scientific environments have been described as unwelcoming to the LGBGQ+ community, and fields like physics systematically documented these challenges, climate in biology workplaces has not assessed. We conducted largest survey date of LGBTQ+ experiences biology, including 1419 biologists across five professional societies, with 486 identifying LGBTQ+. Trans gender non-conforming (TGNC) reported lower belonging morale within workplace, community compared cis, straight biologists. They...
Metabolic engineering has facilitated the production of pharmaceuticals, fuels, and soft materials but is generally limited to optimizing well-defined metabolic pathways. We hypothesized that reaction space available could be expanded by coupling extracellular electron transfer performance an exogenous redox-active metal catalyst. Here we demonstrate electroactive bacterium Shewanella oneidensis can control activity a copper catalyst in atom-transfer radical polymerization (ATRP) via...
The relative scarcity of well-defined genetic and metabolic linkages to material properties impedes biological production inorganic materials. physiology electroactive bacteria is intimately tied transformations, which makes genetically tractable well-studied electrogens, such as Shewanella oneidensis, attractive hosts for synthesis. Notably, this species capable reducing a variety transition-metal ions into functional nanoparticles, but exact mechanisms nanoparticle biosynthesis remain...
Extracellular electron transfer (EET) is a process through which certain microorganisms can electrons across their cell membranes to external acceptors, linking cellular metabolism environment. While Geobacter and Shewanella have been the primary models for EET research, emerging studies reveal that EET-active species are also associated with fermentation human gut microbiome. Leveraging ability of bridge biological electronic systems, we present protocol using organic electrochemical...
Enhancing materials with the qualities of living systems, including sensing, computation, and adaptation, is an important challenge in designing next-generation technologies. Living address this by incorporating live cells as actuating components that control material function. For abiotic materials, requires new methods couple genetic metabolic processes to properties. Toward goal, we demonstrate extracellular electron transfer (EET) from Shewanella oneidensis can be leveraged radical...
Extracellular electron transfer (EET) is an anaerobic respiration process that couples carbon oxidation to the reduction of metal species. In presence a suitable catalyst, EET allows for cellular metabolism control variety synthetic transformations. Here, we report use from electroactive bacterium Shewanella oneidensis metabolic and genetic over Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC). CuAAC conversion under aerobic conditions was dependent on live, actively respiring S. cells....
Organoids are powerful models of tissue physiology, yet their applications remain limited due to relatively simple morphology and high organoid-to-organoid structural variability. To address these limitations we developed a soft, composite yield-stress extracellular matrix that supports optimal organoid morphogenesis following freeform 3D bioprinting cell slurries at tissue-like densities. The material is designed with two temperature regimes: 4 °C it exhibits reversible behavior support...
Abstract Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on variety inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed electrodes. To address this, we developed platform for EET flux from cell suspensions using aqueous dispersions infrared plasmonic tin‐doped indium oxide nanocrystals. Tracking the change in optical extinction...
Three-dimensional (3D) printing can be beneficial to tissue engineers and the regenerative medicine community because of its potential rapidly build elaborate 3D structures from cellular material inks. However, predicting changes structure pattern printed tissues arising mechanical activity constituent cells is technically conceptually challenging. This perspective targeted scientists interested in bioprinting, but point view as mechanically active living materials. The dynamic forces...
Abstract Individual cells direct non-equilibrium processes through coordinated signal transduction and gene expression, allowing for dynamic control over multicellular, system-wide behavior. This behavior extends to remodeling the extracellular polymer matrix that encases biofilms tissues, where constituent dictate spatiotemporal network properties including stiffness, pattern formation, transport properties. The majority of synthetic networks cannot recreate these phenomena due their lack...
The construction of three-dimensional (3D) microvascular networks with defined structures remains challenging. Emerging bioprinting strategies provide a means patterning endothelial cells (ECs) into the geometry 3D networks, but microenvironmental cues necessary to promote their self-organization cohesive and perfusable microvessels are not well known. To this end, we reconstituted microvessel formation in vitro by thin lines closely packed ECs fully embedded within extracellular matrix...
The human antibody repertoire is a unique repository of information regarding infection, inflammation, and autoimmunity the past, present, future. However, antibodies can span vast ranges concentrations with varying affinities often heavily polarized by few species. These complexities lead to difficulties detecting characterizing low abundance species that may be relevant disease. We therefore developed method selectively remove from sample in proportion titer prior analysis, referred as...
Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, neuromorphic computing. Despite their potential, developing programmable modular methods living systems to interface with OECTs has proven challenging. Here we describe hybrid containing the model electroactive bacterium
Abstarct Biological production of inorganic materials is impeded by relatively few organisms possessing genetic and metabolic linkage to material properties. The physiology electroactive bacteria intimately tied transformations, which makes genetically tractable well-studied electrogens, such as Shewanella oneidensis , attractive hosts for synthesis. Notably, this species capable reducing a variety transition-metal ions into functional nanoparticles, but exact mechanisms nanoparticle...
Abstract Extracellular electron transfer (EET) is a critical form of microbial metabolism that enables respiration on variety inorganic substrates, including metal oxides. However, quantifying current generated by electroactive bacteria has been predominately limited to biofilms formed electrodes. To address this, we developed platform for EET flux from cell suspensions using aqueous dispersions infrared plasmonic tin-doped indium oxide nanocrystals. Tracking the change in optical extinction...
Abstract Extracellular electron transfer (EET) is an anaerobic respiration process that couples carbon oxidation to the reduction of metal species. In presence a suitable catalyst, EET allows for cellular metabolism control variety synthetic transformations. Here, we report use from model electroactive bacterium Shewanella oneidensis metabolic and genetic over Cu(I)-catalyzed Alkyne-Azide Cycloaddition (CuAAC). CuAAC conversion under aerobic conditions was dependent on live, actively...
Abstract Enhancing materials with the qualities of living systems, including sensing, computation, and adaptation, is an important challenge in designing next-generation technologies. Living seek to address this by incorporating live cells as actuating components that control material function. For abiotic materials, requires new methods couple genetic metabolic processes properties. Toward goal, we demonstrate extracellular electron transfer (EET) from Shewanella oneidensis can be leveraged...