A5071659132- Mycobacterium research and diagnosis
- Neutropenia and Cancer Infections
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Digital Imaging for Blood Diseases
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- 3D Printing in Biomedical Research
- Plant Pathogens and Fungal Diseases
- Cell Image Analysis Techniques
- Advanced biosensing and bioanalysis techniques
- Image Processing Techniques and Applications
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Protein purification and stability
- Respiratory viral infections research
- Malaria Research and Control
- Phytochemistry and Bioactive Compounds
- Viral Infectious Diseases and Gene Expression in Insects
Chan Zuckerberg Initiative (United States)
2020-2025
Abstract We evaluated the performance of Abbott BinaxNOW rapid antigen test for coronavirus disease 2019 (Binax-CoV2) to detect virus among persons, regardless symptoms, at a public plaza site ongoing community transmission. Titration with cultured severe acute respiratory syndrome 2 yielded human observable threshold between 1.6 × 104-4.3 104 viral RNA copies (cycle [Ct], 30.3–28.8). Among 878 subjects tested, 3% (26 878) were positive by reverse-transcription polymerase chain reaction,...
ABSTRACT We evaluated the performance of Abbott BinaxNOW™ Covid-19 rapid antigen test to detect virus among persons, regardless symptoms, at a public plaza site ongoing community transmission. Titration with cultured clinical SARS-CoV-2 yielded human observable threshold between 1.6×10 4 -4.3×10 viral RNA copies (cycle (Ct) 30.3-28.8 in this assay). Among 878 subjects tested, 3% (26/878) were positive by RT-PCR, which 15/26 had Ct<30, indicating high load. 40% (6/15) Ct<30...
Organoids are powerful models of tissue physiology, yet their applications remain limited due to relatively simple morphology and high organoid-to-organoid structural variability. To address these limitations we developed a soft, composite yield-stress extracellular matrix that supports optimal organoid morphogenesis following freeform 3D bioprinting cell slurries at tissue-like densities. The material is designed with two temperature regimes: 4 °C it exhibits reversible behavior support...
Manual microscopic inspection of fixed and stained blood smears has remained the gold standard for Plasmodium parasitemia analysis over a century. Unfortunately, smear preparation consumes time reagents, while manual microscopy is skill-dependent labor-intensive. Here, we demonstrate that deep learning enables both life stage classification accurate quantification ordinary brightfield images live, unstained red cells. We tested our method using light microscope equipped with visible...
Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although light well-established centralized laboratory settings, cost, size, environmental requirements high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present...
Malaria diagnostic testing in high transmission settings remains a burden on healthcare systems. Here we present Remoscope, portable automated imaging cytometer that scans fresh, unstained whole blood using custom neural network low-cost hardware. By screening up to two million red cells, Remoscope performs label-free quantitative stage-specific detection of
Tissue culture in 96-well microplates is conventionally a tedious, highly manual process subject to human error and sensitive individual technique. Here, we describe the Automated Cell Culture Splitter (ACCS), versatile low-cost system for passaging plates of adherent or suspension cells, routine maintenance more specialized downstream applications such as seeding microscopy. The built around Opentrons OT-2 liquid handling robot incorporates novel on-deck imaging-based cell counting...
Abstract Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although light well-established centralized laboratory settings, cost, size, environmental requirements high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here...
Abstract Manual microscopic inspection of fixed and stained blood smears has remained the gold standard for Plasmodium parasitemia analysis over a century. Unfortunately, smear preparation consumes time reagents, while manual microscopy is skill-dependent labor-intensive. Here, we demonstrate that label-free combined with deep learning enables both life stage classification accurate quantification. Using custom-built microscope, find deep-ultraviolet light enhances image contrast resolution,...