A5013846127- Parasitic Infections and Diagnostics
- Malaria Research and Control
- Amoebic Infections and Treatments
- Mosquito-borne diseases and control
- Parasitic infections in humans and animals
- Toxoplasma gondii Research Studies
- Coccidia and coccidiosis research
- Enterobacteriaceae and Cronobacter Research
- Gut microbiota and health
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Computational Drug Discovery Methods
- Erythrocyte Function and Pathophysiology
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Digital Imaging for Blood Diseases
- Blood properties and coagulation
- Complement system in diseases
- Infant Nutrition and Health
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Venomous Animal Envenomation and Studies
- Parasites and Host Interactions
- Vibrio bacteria research studies
National University of Singapore
2016-2025
Guangxi Medical University
2025
Guangxi Medicinal Botanical Garden
2025
University of Illinois System
2024
National University Hospital
2023
University of Otago
2019-2020
Singapore Science Park
2012-2018
Singapore Immunology Network
2017
National University Health System
2017
Agriculture and Food
2017
The mechanism of action artemisinin and its derivatives, the most potent anti-malarial drugs, is not completely understood. Here we present an unbiased chemical proteomics analysis to directly explore this in Plasmodium falciparum. We use alkyne-tagged analogue coupled with biotin identify 124 covalent binding protein targets, many which are involved essential biological processes parasite. Such a broad targeting spectrum disrupts biochemical landscape parasite causes death. Furthermore,...
Laminins and their integrin receptors are implicated in epithelial cell differentiation progenitor maintenance. We report here that a previously unrecognized subpopulation of mouse alveolar cells (AECs) expressing the laminin receptor α6β4, but little or no pro-surfactant C (pro-SPC), is endowed with regenerative potential. Ex vivo, this expanded clonally as progenitors also differentiated toward mature types. Integrin β4 itself was not required for AEC proliferation differentiation. An vivo...
In blood vessels with luminal diameter less than 300μm, red cells (RBCs) which are smaller in size and more deformable leukocytes, migrate to the axial centre of vessel due flow velocity gradient within vessels. This phenomenon displaces leukocytes wall is aptly termed as margination. Here, we demonstrate using microfluidics that stiffer malaria-infected RBCs (iRBCs) behave similar undergo margination towards sidewalls. provides better understanding hemodynamic effects iRBCs microcirculation...
Excess hydrogen peroxide (H2O2) is produced in the pathogenesis of brain injuries and neurodegenerative diseases. H2O2 may damage cells through direct oxidation lipids, proteins DNA or it can act as a signaling molecule to trigger intracellular pathways leading cell death. In this study, caused plasma membranes primary astrocytes become more gel-like, while artificial vesicles composed rat lipid extract became liquid crystalline-like. Besides effects on membrane phase properties, promoted...
During intraerythrocytic development, Plasmodium falciparum exports proteins that interact with the host cell plasma membrane and subplasma membrane-associated spectrin network. Parasite-exported modify mechanical properties of RBCs, resulting in altered circulation. In this work, optical tweezers experiments at normal physiological febrile temperatures are coupled, for first time, targeted gene disruption techniques to measure effect a single parasite-exported protein on RBC deformability....
Blastocystis is a common gut eukaryote detected in humans and animals. It has been associated with gastrointestinal disease the past although recent metagenomic studies also suggest that it member of normal microbiota. This study investigates interactions between pathogenic human isolates belonging to subtype 7 (ST7) bacterial representatives Generally, ST7 exerts positive effect on viability representative bacteria except Bifidobacterium longum. Gene expression analysis flow cytometry...
Blastocystis is a common gut protistan parasite in humans and animals worldwide, but its interrelationship with the host microbiota mucosal immune responses remains poorly understood. Different murine models of colonization were used to examine effect subtype (ST4) on microbial community adaptive system.Blastocystis ST4-colonized normal healthy mice Rag1-/- asymptomatically was able alter composition, mainly leading increases proportion Clostridia vadinBB60 group Lachnospiraceae NK4A136...
Rationale:The gut microbiota plays a significant role in the pathogenesis of inflammatory bowel disease (IBD).However, Blastocystis infection and Blastocystis-altered development diseases their underlying mechanisms are not well understood.Methods: We investigated effect ST4 ST7 on intestinal microbiota, metabolism, host immune responses, then explored microbiome dextran sulfate sodium (DSS)-induced colitis mice.Results: This study showed that prior colonization with conferred protection...
Abstract The large intestine harbors microorganisms playing unique roles in host physiology. beneficial or detrimental outcome of host‐microbiome coexistence depends largely on the balance between regulators and responder intestinal CD4 + T cells. We found that ulcerative colitis‐like changes after infection with protist Blastocystis ST7 a mouse model are associated reduction anti‐inflammatory Treg cells simultaneous expansion pro‐inflammatory Th17 responders. These alterations depended...
Abstract Blastocystis is a species complex that exhibits extensive genetic diversity, evidenced by its classification into several genetically distinct subtypes (ST). Although studies have shown the relationships between specific subtype and gut microbiota, there no study to show effect of ubiquitous ST1 on microbiota host health. Here, we colonization increased proportion beneficial bacteria Alloprevotella Akkermansia , induced Th2 Treg cell responses in normal healthy mice. ST1-colonized...
Blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and wide range animals. Evidence accumulated over last decade suggests association with gastrointestinal disorders involving diarrhea, abdominal pain, constipation, nausea, fatigue. Clinical experimental studies have associated inflammation, it has been shown that potential to modulate host immune response. also reported be an opportunistic pathogen immunosuppressed patients, especially those suffering...