A5054279862- Malaria Research and Control
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Invertebrate Immune Response Mechanisms
- Trypanosoma species research and implications
- Genetic and Kidney Cyst Diseases
- HIV Research and Treatment
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Microtubule and mitosis dynamics
- Toxoplasma gondii Research Studies
- Research on Leishmaniasis Studies
- Hepatitis C virus research
- Heat shock proteins research
- Monoclonal and Polyclonal Antibodies Research
- Yersinia bacterium, plague, ectoparasites research
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Herpesvirus Infections and Treatments
- Prenatal Screening and Diagnostics
- Complement system in diseases
- Lipid metabolism and biosynthesis
- Hemoglobinopathies and Related Disorders
- Genomics and Phylogenetic Studies
Institut Pasteur
2014-2025
Inserm
2015-2025
Université Paris Cité
2023-2024
Centre National de la Recherche Scientifique
1995-2014
HIA du Val-de-Grâce à Paris
2008
Bipar
2002
Stanford University
1997-2001
Institute of Parasitology
1994
Toxoplasma gondii is a common human pathogen causing serious, even fatal, disease in the developing fetus and immunocompromised patients. Despite its ability to reproduce sexually broad geographic host range, has clonal population structure comprised principally of three lines. We have analyzed 15 polymorphic loci archetypal type I, II, III strains found that polymorphism was limited to, at most, two rather than allelic classes no detected between alleles given type. Multilocus analysis 10...
"Age-Dependent Carriage of Multiple Plasmodium falciparum Merozoite Surface Antigen-2 Alleles in Asymptomatic Malaria Infections" published on Jan 1995 by The American Society Tropical Medicine and Hygiene.
During intraerythrocytic development, Plasmodium falciparum exports proteins that interact with the host cell plasma membrane and subplasma membrane-associated spectrin network. Parasite-exported modify mechanical properties of RBCs, resulting in altered circulation. In this work, optical tweezers experiments at normal physiological febrile temperatures are coupled, for first time, targeted gene disruption techniques to measure effect a single parasite-exported protein on RBC deformability....
"Different Genetic Characteristics of Plasmodium falciparum Isolates Collected During Successive Clinical Malaria Episodes in Senegalese Children" published on Jun 1996 by The American Society Tropical Medicine and Hygiene.
We report on an analysis of the constraints PCR typing field Plasmodium falciparum isolates by using a few highly polymorphic markers, MSA-1, MSA-2, TRAP, and CS. show that reactions are specific for P. species. The detection threshold (minimum number parasites required to detect visible band ethidium bromide) differed from one marker other and, within locus, primer combination other. Importantly, various MSA-1 MSA-2 reference alleles were amplified with same efficiency. Amplification...
Proteins exported by Plasmodium falciparum to the red blood cell (RBC) membrane modify structural properties of parasitized RBC (Pf-RBC). Although quasi-static single assays show reduced ring-stage Pf-RBCs deformability, parameters influencing their microcirculatory behavior remain unexplored. Here, we study dynamic and role parasite protein Pf155/Ring-Infected Erythrocyte Surface Antigen (RESA). Diffraction phase microscopy revealed RESA-driven decreased fluctuations. Microfluidic...
Toxoplasma gondii has recently come under intense study as a model for intracellular parasitism because it number of properties that facilitate experimental manipulation. Attention is now being turned towards understanding the developmental biology this complex parasite. The differentiation between two asexual stages, rapidly growing tachyzoites and more slowly dividing, encysted bradyzoites, particular interest. Progression from former to latter influenced by host's immune response. This...
The iron-regulated irp2 gene is specific for the highly pathogenic Yersinia species and encodes high-molecular-weight protein 2 (HMWP2). Despite established correlation between presence of HMWP2 virulence, role this still unknown. To gain insight into function HMWP2, entire coding sequence promoter were sequenced. Two putative -35 -10 sequences identified upstream a large open reading frame, two potential Fur-binding sites found overlapping second box. frame composed 6,126 nucleotides may...
Summary A gene encoding a previously undescribed antigen of Plasmodium falciparum has been isolated from genomic expression library by use pool human immune sera. Northern blot analysis indicated that the is expressed at late stages intra–erythrocytic cycle. This antigen, 332, contains series degenerated amino acid repeats. Human antibodies affinity–purified on 332 recombinant reacted with family parasite proteins are products different genes. We identified antigens 11.1 and Pf 155–RESA as...
Artesunate, the recommended drug for severe malaria, rapidly clears malaria parasite from infected patients but frequently induces anemia-called post-artesunate delayed hemolysis (PADH)-for which a simple predictive test is urgently needed. The underlying event in PADH expulsion of artesunate-exposed parasites their host erythrocytes by pitting. We show that histidine-rich protein 2 (HRP2) Plasmodium falciparum persists circulation artesunate-treated Bangladesh and French travelers who...
Primary defects in motile cilia result dysfunction of the apparatus responsible for generating fluid flows. Defects these mechanisms underlie disorders characterized by poor mucus clearance, resulting susceptibility to chronic recurrent respiratory infections, often associated with infertility; laterality occur about 50% such individuals. Here we report biallelic variants LRRC56 (known as oda8 Chlamydomonas) identified three unrelated families. The phenotype comprises and pulmonary...
ABSTRACT Intraflagellar transport (IFT) is required for construction of most cilia and flagella. Here, we used electron microscopy, immunofluorescence live video microscopy to show that IFT absent or arrested in the mature flagellum Trypanosoma brucei upon RNA interference (RNAi)-mediated knockdown IFT88 IFT140, respectively. Flagella assembled prior RNAi did not shorten, showing essential maintenance flagella length. Although ultrastructure axoneme was visibly affected, flagellar beating...
Intraflagellar transport (IFT) is the rapid bidirectional movement of large protein complexes driven by kinesin and dynein motors along microtubule doublets cilia flagella. In this study, we used a combination high-resolution electron light microscopy to investigate how where these IFT trains move within flagellum protist Trypanosoma brucei. Focused ion beam scanning (FIB-SEM) analysis trypanosomes showed that are found almost exclusively two sets (3–4 7–8) distribute in categories according...
Summary During erythrocyte invasion, the Plasmodium falciparum Ring‐infected surface antigen (RESA) establishes specific interactions with spectrin. Based on analysis of strains a large chromosome 1 deletion, RESA has been assigned several functions, none which is firmly established. Analysis parasites disrupted resa1 gene and isogenic parental or resa3 ‐disrupted controls confirmed critical role in reactivity immune adult sera glutaraldehyde‐fixed ring stages. Absence did not influence...
Abstract Background Translation of the genome sequence Plasmodium sp . into biologically relevant information relies on high through-put genomics technology which includes transcriptome analysis. However, few studies to date have used this powerful approach explore alterations P. falciparum parasites exposed antimalarial drugs. Results The rapid action artesunate allowed us study dynamic changes parasite in synchronous cultures drug for 90 minutes and 3 hours. Developmentally regulated genes...
The protozoan parasite Trypanosoma brucei is a mono-flagellated cell during the G1-phase of its cycle. In order to duplicate, it assembles new flagellum alongside mature one, in which further elongation prevented. Our group proposed model where locked after construction full length (Bertiaux et al. 2018) and access building blocks for exclusive flagellum. To test this hypothesis directly, we developed tool inducible expression tagged tubulin. Alpha-tubulin that was with an intragenic...
ABSTRACT Tubulin polyglutamylation is a prominent feature of eukaryotic cilia and flagella. In the protist parasite Trypanosoma brucei , nine different tubulin tyrosine ligase-like (TTLL) enzymes are potentially involved in glutamylation. Given this enzymatic diversity, it important to qualitatively quantitatively characterize polyglutamylation, order understand trypanosome code pave way for functional assignments TTLLs. Robust mass spectrometry (MS) based proteomics approaches analysis...
A genomic library containing DNA fragments of 0.5 to 2 kilobase pairs in length from Yersinia enterocolitica serovar O:8 was constructed a bacteriophage lambda gt11 expression vector. Mouse antibodies specific for the iron-regulated high-molecular-weight proteins (HMWPs) were used screen library. Two positive clones 1 and pairs, designated A13 D7, respectively, detected isolated. They coded beta-galactosidase fusion 151,000 138,000 daltons (Da). Antibodies affinity purified on two...
Protein phosphatase types 1 (PP1) and 2A (PP2A) represent two major families of serine/threonine protein phosphatases that have been implicated in the regulation many cellular processes, including cell growth apoptosis mammalian cells. PP1 PP2A proteins are composed oligomeric complexes comprising a catalytic structure (PP1c or PP2AC) containing enzymatic activity at least one more interacting subunit. The binding different subunits to generates broad variety holoenzymes. We showed here...
The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to hypothesis might be raft-forming lipids. However, a detailed lipidomic analysis flagellar is not available. Novel protocols detach isolate intact from Trypanosoma brucei procyclic forms combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us determine phospholipid composition relative whole cells. Our analyses revealed phosphatidylethanolamine,...
The single flagellum of African trypanosomes is essential in multiple aspects the parasites' development. FLAgellar Member 8 protein (FLAM8), localised to tip cultured insect forms Trypanosoma brucei, was identified as a marker locking event that controls length. Here, we investigated whether FLAM8 could also reflect maturation state other parasite cycle stages. We observed distribution extended along entire flagellar cytoskeleton mammalian-infective forms. Then, rapid concentration distal...