A5100368987- Cardiac Valve Diseases and Treatments
- Aortic Disease and Treatment Approaches
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Congenital Heart Disease Studies
- Connective tissue disorders research
- Infective Endocarditis Diagnosis and Management
- Cardiomyopathy and Myosin Studies
- Animal Genetics and Reproduction
- Aortic aneurysm repair treatments
- MicroRNA in disease regulation
- Innovative Energy Harvesting Technologies
- Cardiovascular Issues in Pregnancy
- Hip and Femur Fractures
- Endoplasmic Reticulum Stress and Disease
- Coronary Artery Anomalies
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Xenotransplantation and immune response
- Tracheal and airway disorders
- Vasculitis and related conditions
- Cardiovascular and Diving-Related Complications
- RNA Interference and Gene Delivery
Second Military Medical University
2012-2023
Changhai Hospital
2018-2023
Wuhan University
2022
Renmin Hospital of Wuhan University
2022
Jiangsu Province Hospital
2022
Henan Cancer Hospital
2022
Xinjiang Academy of Agricultural Sciences
2022
Nanjing General Hospital of Nanjing Military Command
2012-2022
Chengdu Sport University
2022
Pingdingshan University
2022
Implantable medical devices are widely used for monitoring and treatment of severe diseases. In particular, an implantable cardiac pacemaker is the most effective therapeutic device treating bradyrhythmia, however its surgical replacement inevitable every 5–12 years due to limited life built-in battery. Although several approaches energy harvesting have been explored in this decade powering pacemakers, modern, commercial, full-function has never powered effectively yet. Here, we report...
Various detergent-based protocols are used to remove cells and cellular debris in porcine aortic valve (PAV). However, the removal of antigenic components has not been thoroughly elucidated date. In this study, we 4 decellularize PAVs aimed evaluate their effects on removing components.Porcine valves were decellularized using sodium dodecyl sulfate (SDS), SDS combination with deoxycholate (SDS/SD), Triton X-100, X-100 SD (Triton X-100/SD), respectively. Untreated as controls....
Induced pluripotent stem (iPS) cell technology demonstrates that somatic cells can be reprogrammed to a state by over-expressing four reprogramming factors. This has created an interest in deriving iPS from domesticated animals such as pigs, sheep and cattle. Moloney murine leukemia retrovirus vectors have been widely used generate study mouse cells. However, this system infects only rat cells, which limits its use establishing other mammals. In our study, we demonstrate novel strategy...
Calcific aortic valve disease (CAVD) is an active pathological process mediated by abnormal activation and transdifferentiation of valvular interstitial cells (VICs). The present study aims to investigate the function underlying mechanism basic fibroblast growth factor (BFGF) on osteogenic differentiation VICs. Porcine VICs cultured with induction medium are supplemented or without BFGF. Morphology identified fluorescein isothiocyanate-labeled phalloidin, cell viability assessed counting...
Calcific aortic valve disease (CAVD) is a common heart in aging populations, and aberrant osteogenic differentiation of valvular interstitial cells (VICs) plays critical role the pathogenesis ectopic ossification valve. miR-214 has been validated to be involved osteogenesis process. Here, we aim investigate mechanism CAVD progression. expression was significantly downregulated leaflets, accompanied by upregulation markers. Overexpression suppressed VICs, while silencing promoted this...
Background . Tbx18 is a vital transcription factor involved in embryonic sinoatrial node (SAN) formation process but gradually vanished after birth. Myocardial injection of lentiviral converts cardiomyocytes into pacemaker-like cells morphologically and functionally. In this vitro vivo study, genetical modification porcine bone mesenchymal stem (BMSCs) by recapturing the expression creates biological pacemaker which was examined. Methods The isolated BMSCs were transfected with Tbx18,...
This study aimed to determine the mechanism of isogeneic-induced pluripotent stem cells (iPSCs) homing vascular transplants and their therapeutic effect on chronic allogeneic vasculopathy. We found that integrin β1 (Intgβ1) was dominant β unit in iPSCs mediates adhesion circulatory endothelial (ECs). Intgβ1 knockout or Intgβ1-siRNAs inhibit iPSC migration across activated monolayers. The effects following were examined: iPSCs, Intgβ1-knockout transfected with nontargeting siRNAs,...
Abstract Although a variety of reprogramming strategies have been reported to create transgene-free induced pluripotent stem (iPS) cells from differentiated cell sources, fundamental question still remains: Can we generate safe iPS that the full spectrum features corresponding embryonic (ES) cells? Studies in mouse indicated positive answer this question. However, reality is no other species derived line can truly mimic ES quality. Specifically, critical data for chimera formation and...
Postoperative hypoxemia is a high-risk complication after acute type A aortic dissection (TAAD) surgery. Prone position (PP) an effective treatment for respiratory failure, which may improve the gas exchange of injured lung and patient's survival. PP reported to condition cardiac However, limited data exist on effect in patients who underwent TAAD surgery.We retrospectively analyzed clinical outcomes seven with severe The results arterial blood gas, chest X-ray, survival were collected.Seven...
Multiple of subsequent procedures may necessary in Marfan syndrome (MFS) patients after initial surgery. The aim this study was to investigate the full spectrum secondary distal vascular or valvular interventions encountered surgery.Retrospective analysis 201 consecutive MFS between January 2000 and March 2019 who underwent 274 aortic reinterventions 5 mitral valve replacements.Of enrolled (73 female, mean age 37.0±12.8 years), surgical indication for 93 root aneurysm, another 108...
Data on outcome of Stanford type A aortic dissection (TAAD) in Marfan syndrome (MFS) patients are limited. We investigated the full spectrum reoperation and survival after initial surgery MFS who suffered TAAD.Retrospective analysis 85 consecutive one-single center during past 15 years.Overall, with TAAD underwent surgical repair [74% acute dissections; 80% DeBakey I; 91% composite valved graft; 70% total arch replacement (TAR); 68% frozen elephant trunk (FET); 7% in-hospital mortality] at...
Background: Defecation delay is a common symptom in patients after tricuspid valve replacement (TVR). Previous studies have demonstrated that defecation was associated with worse clinical outcomes of critically ill patients. Our study aimed to investigate the incidence and risk factors TVR its adverse outcomes. Methods: A retrospective conducted 206 undergoing under cardiopulmonary bypass from May 2005 July 2021. According first postoperative time surgery, were divided into delayed group...
Calcific aortic valve disease (CAVD)was once recognized as a passive degenerative disease, but it has recently been shown to be an active process predominately regulated by valvular interstitial cells (VICs), which maintain the integrity of heart valve. In vitro culture VICs will lead phenotypic switching into osteoblast-like and form calcific nodules, can influenced multiple extrinsic factors. This paper provides systematical overview established in models CAVD. Key words: Calcific...
Although the mammalian heart had been recognized as a post-mitotic non-regenerative organ, recent evidence demonstrated measurable cardiomyocyte (CM) renewal at low rates in normal hearts and augmented post-injury myocardium. However, resources cellular processes of such new CM formation, underlying molecular programs remained largely undetermined. While CMs neonatal or adult tissue culture can undergo (partial) dedifferentiation proliferation, roles vivo cycling were unclear. Here, we...