A5062503131- Plant Molecular Biology Research
- Plant-derived Lignans Synthesis and Bioactivity
- Global trade and economics
- Plant nutrient uptake and metabolism
- Consumer Market Behavior and Pricing
- Bayesian Methods and Mixture Models
- COVID-19 Pandemic Impacts
- Plant Reproductive Biology
- COVID-19 epidemiological studies
- Firm Innovation and Growth
- Statistical Methods and Inference
- Economic Growth and Productivity
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Phytochemistry and Biological Activities
- Corporate Finance and Governance
- COVID-19 and Mental Health
- Traditional and Medicinal Uses of Annonaceae
- Photosynthetic Processes and Mechanisms
- Economics of Agriculture and Food Markets
- Monetary Policy and Economic Impact
- Banking stability, regulation, efficiency
- Plant Parasitism and Resistance
- Magnolia and Illicium research
- Global Trade and Competitiveness
Niigata University
2022-2024
University of British Columbia
2012-2023
RIKEN Center for Sustainable Resource Science
2013-2022
Tokyo University of Agriculture and Technology
2016-2022
Niigata Prefectural Central Hospital
2022
University of British Columbia Hospital
2021
Hitotsubashi University
2017-2019
Fujifilm (Japan)
2008-2015
Japan Science and Technology Agency
2013-2015
Western University
2004-2009
The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as major for more than 70 y. Although several pathways have proposed, how is synthesized plants still unclear. Previous genetic enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) YUCCA (YUC) flavin monooxygenase-like proteins are required biosynthesis IAA during development, but these enzymes were placed two...
Auxin is an essential hormone, but its biosynthetic routes in plants have not been fully defined. In this paper, we show that the TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family of amino transferases converts tryptophan to indole-3-pyruvate (IPA) and YUCCA (YUC) flavin monooxygenases participates converting IPA indole-3-acetic acid, main auxin plants. Both YUCs TAAs shown play roles biosynthesis, it has suggested they participate two independent pathways. Here, all taa mutant...
Auxins are hormones that regulate many aspects of plant growth and development. The main auxin is indole-3-acetic acid (IAA), whose biosynthetic pathway not fully understood. Indole-3-acetaldoxime (IAOx) has been proposed to be a key intermediate in the synthesis IAA several other indolic compounds. Genetic studies biosynthesis Arabidopsis have suggested 2 distinct pathways involving CYP79B or YUCCA ( YUC ) genes may contribute IAOx also involved conversion IAA. Here we report biochemical...
Auxin plays an essential role in root development. It has been a long-held dogma that auxin required for development is mainly transported from shoots into roots by polarly localized transporters. However, it known also synthesized roots. Here we demonstrate group of YUCCA (YUC) genes, which encode the rate-limiting enzymes biosynthesis, Arabidopsis Five YUC genes (YUC3, YUC5, YUC7, YUC8 and YUC9) display distinct expression patterns during Simultaneous inactivation five (yucQ mutants) leads...
The functions of microRNAs and their target mRNAs in Arabidopsis thaliana development have been widely documented; however, roles stress-responsive targets are not as well understood. Using small RNA deep sequencing ATH1 microarrays to profile mRNAs, we identified IAA-Ala Resistant3 (IAR3) a new miR167a. As expected, IAR3 mRNA was cleaved at the miR167a complementary site under high osmotic stress levels decreased, whereas increased. hydrolyzes an inactive form auxin (indole-3-acetic acid...
This paper evaluates the dynamic impact of various policies adopted by US states on growth rates confirmed Covid-19 cases and deaths as well social distancing behavior measured Google Mobility Reports, where we take into consideration people's voluntarily behavioral response to new information transmission risks. Our analysis finds that both risks are important determinants shows a change in explains large fraction observed changes behavior. counterfactual experiments suggest nationally...
In dynamic discrete choice analysis, controlling for unobserved heterogeneity is an important issue, and finite mixture models provide flexible ways to account it. This paper studies nonparametric identifiability of type probabilities type-specific component distributions in choices. We derive sufficient conditions identification various choices used applied work under different assumptions on the Markov property, stationarity, type-invariance transition process. Three elements emerge as...
Gibberellins (GAs) are diterpene plant hormones essential for many developmental processes. Although the GA biosynthesis pathway has been well studied, our knowledge on its early stage is still limited. There two possible routes of isoprenoids leading to GAs, mevalonate (MVA) in cytosol and methylerythritol phosphate (MEP) plastids. To distinguish these possibilities, metabolites from each isoprenoid were selectively labeled with<sup>13</sup>C <i>Arabidopsis</i> seedlings. Efficient...
Plants produce the common isoprenoid precursors isopentenyl diphosphate and dimethylallyl (DMAPP) through methylerythritol phosphate (MEP) pathway in plastids mevalonate (MVA) cytosol. To assess which pathways contribute DMAPP for cytokinin biosynthesis, metabolites from each were selectively labeled with (13)C Arabidopsis seedlings. Efficient labeling was achieved by blocking endogenous genetically or chemically during feed of a precursor specific to MEP MVA pathways. Liquid...
Pinoresinol-lariciresinol and isoflavone reductase classes are phylogenetically related, as is a third, the so-called "isoflavone homologs." This study establishes first known catalytic function for latter, being able to engender NADPH-dependent reduction of phenylcoumaran benzylic ethers. Accordingly, all three involved in biosynthesis important related phenylpropanoid-derived plant defense compounds. In this investigation, ether from gymnosperm, <i>Pinus taeda</i>, was cloned, with...
Summary Indole‐3–acetic acid ( IAA ), an auxin plant hormone, is biosynthesized from tryptophan. The indole‐3–pyruvic IP y A ) pathway, involving the tryptophan aminotransferase TAA 1 and YUCCA YUC enzymes, was recently found to be a major biosynthetic pathway in Arabidopsis. catalyzes conversion of , produces . Using chemical biology approach with maize coleoptiles, we identified 5–(4–chlorophenyl)‐4H‐1,2,4–triazole‐3–thiol (yucasin) as potent inhibitor biosynthesis ‐expressing coleoptile...
Auxin regulates every aspect of plant growth and development. Previous genetic studies demonstrated that YUCCA (YUC) flavin-containing monooxygenases (FMOs) catalyze a rate-limiting step in auxin biosynthesis YUCs are essential for many developmental processes. We proposed convert indole-3-pyruvate (IPA) to indole-3-acetate (IAA). However, the exact biochemical mechanism has remained elusive. Here we present characterization recombinant Arabidopsis YUC6. Expressed purified from Escherichia...
In Arabidopsis thaliana , lateral root (LR) formation is regulated by multiple auxin/indole-3-acetic acid (Aux/IAA)–AUXIN RESPONSE FACTOR (ARF) modules: (i) the IAA28–ARFs module regulates LR founder cell specification; (ii) SOLITARY-ROOT (SLR)/IAA14–ARF7–ARF19 nuclear migration and asymmetric divisions of cells for initiation; (iii) BODENLOS/IAA12–MONOPTEROS/ARF5 also initiation organogenesis. The number Aux/IAA–ARF modules involved in remains unknown. this study, we isolated shy2-101...
The plant hormone auxin (indole-3-acetic acid [IAA]) has previously been suggested to regulate diverse forms of dormancy in both seed plants and liverworts. Here, we use loss- gain-of-function alleles for synthesis- signaling-related genes, as well pharmacological approaches, study how regulates development the gametophyte generation liverwort Marchantia polymorpha. We found that M. polymorpha possess smallest known toolkit indole-3-pyruvic (IPyA) pathway any land this synthesis mainly is...
The phytohormone auxin plays a central role in many aspects of plant growth and development. IAA is the most studied natural that possesses property polar transport plants. Phenylacetic acid (PAA) has also been recognized as for >40 years, but its development remains unclear. In this study, we show PAA have overlapping regulatory roles distinct characteristics auxins widely distributed vascular non-vascular Although biological activities are lower than those IAA, endogenous levels much...
Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. develop a multicellular infectious organ called haustorium after recognition of host-released signals. To understand molecular events associated with host signal perception and development, we identified differentially regulated genes expressed during early development facultative parasite Phtheirospermum japonicum using de novo assembled transcriptome customized microarray. Among that were upregulated YUC3,...
Despite plants infected by pathogens are often unable to produce offspring, it remains unclear how sterility is induced in host plants. In this study, we demonstrate that TENGU, a phytoplasmal virulence peptide known as dwarfism inducer, acts an inducer of sterility. Transgenic expression TENGU both male and female Arabidopsis thaliana flowers similar those observed double knockout mutants auxin response factor 6 (ARF6) ARF8, which regulate floral development jasmonic acid (JA)-dependent...
This paper develops an open economy model with heterogeneous final goods producers who simultaneously choose whether to export their and use imported intermediates. The highlights mechanisms whereby import policies affect aggregate productivity, resource allocation, industry activity along both the extensive intensive margins. Using theoretical model, we develop estimate a structural empirical that incorporates heterogeneity in productivity shipping costs using Chilean plant-level data for...
Agrobacterium tumefaciens infects plants and induces the formation of tumors called “crown galls” by integrating transferred-DNA (T-DNA) region Ti-plasmid into plant nuclear genome. Tumors are formed because T-DNA encodes enzymes that modify synthesis two growth hormones, auxin cytokinin (CK). Here, we show a CK biosynthesis enzyme, Tmr, which is encoded region, targeted to functions in plastids infected cells, despite having no typical plastid-targeting sequence. Evidence provided Tmr an...
Isopentenyl/dimethylallyl diphosphate isomerase (IPI) catalyzes the interconversion of isopentenyl (IPP) and dimethylallyl (DMAPP), which are universal C(5) units isoprenoids. In plants, IPP DMAPP synthesized via cytosolic mevalonate (MVA) plastidic methylerythritol phosphate (MEP) pathways, respectively. However, role IPI in each pathway plant development is unknown due to a lack genetic studies using IPI-defective mutants. Here, we show that atipi1atipi2 double mutant, defective two...