A5100756206- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Hippo pathway signaling and YAP/TAZ
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Protein Tyrosine Phosphatases
- Animal Virus Infections Studies
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- Virology and Viral Diseases
- Inflammatory mediators and NSAID effects
- Mosquito-borne diseases and control
- Signaling Pathways in Disease
- Lipid metabolism and biosynthesis
- Galectins and Cancer Biology
- Ferroptosis and cancer prognosis
- Herpesvirus Infections and Treatments
- Wnt/β-catenin signaling in development and cancer
- Cancer Research and Treatments
- Natural product bioactivities and synthesis
Dalian Medical University
2015-2024
Zhongda Hospital Southeast University
2023
Second Affiliated Hospital of Dalian Medical University
2015-2021
First Affiliated Hospital of Dalian Medical University
2015
Institute of Biophysics
2013
Chinese Academy of Sciences
2013
China Agricultural University
2013
University of Science and Technology of China
2013
Yangzhou University
2007-2012
State Key Laboratory of Veterinary Biotechnology
2011
Transcription coactivator Yes-associated protein (YAP) plays an important role in the regulation of cell proliferation and apoptosis. Here, we identify a new YAP cellular senescence. We find that expression levels proteins decrease following replication-induced senescence IMR90 cells. Silencing inhibits induces premature In additional experiments, observe induced by deficiency is TEAD- Rb/p16/p53-dependent. Furthermore, show Cdk6 direct downstream target gene senescence, through YAP-TEAD...
Abstract Hepatocellular carcinoma (HCC) is refractory to chemotherapies, necessitating novel effective agents. The lysosome inhibitor Bafilomycin A1 (BafA1) at high concentrations displays cytotoxicity in a variety of cancers. Here we show that BafA1 nanomolar suppresses HCC cell growth both 2 dimensional (2D) and 3D cultures. induced cycle arrest the G1 phase triggered Cyclin D1 turnover cells dual-specificity tyrosine phosphorylation-regulated kinase 1B (DYRK1B) dependent manner. Notably,...
ABSTRACT Newcastle disease virus (NDV) is an important avian pathogen. We previously reported that NDV triggers autophagy in U251 glioma cells, resulting enhanced replication. In this study, we investigated whether chicken cells and tissues to enhance demonstrated infection induced steady-state chicken-derived DF-1 primary embryo fibroblast (CEF) evident through increased double- or single-membrane vesicles, the accumulation of green fluorescent protein (GFP)-LC3 dots, conversion LC3-I...
Radioresistance is the major cause of cancer treatment failure. Additionally, splicing dysregulation plays critical roles in tumorigenesis. However, involvement alternative resistance cells to radiotherapy remains elusive. We sought investigate key role factor SRSF1 radioresistance lung cancer.Lung cell lines, xenograft mice models, and RNA-seq were employed study detailed mechanisms radioresistance. Clinical tumor tissues TCGA dataset utilized determine expression levels distinct...
Alternative splicing is a critical process to generate protein diversity. However, whether and how alternative regulates autophagy remains largely elusive. Here we systematically identify the factor SRSF1 as an suppressor. Specifically, inhibits autophagosome formation by reducing accumulation of LC3-II numbers autophagosomes in different cell lines. Mechanistically, promotes long isoform Bcl-x that interacts with Beclin1, thereby dissociating Beclin1-PIK3C3 complex. In addition, also...
Non-small cell lung cancer (NSCLC) is the most common type of with a poor prognosis. We previously found that protein disulfide isomerase family 6 (PDIA6) upregulated in squamous carcinoma (LSCC). This study aimed to elucidate clinical relevance, biological functions, and molecular mechanisms PDIA6 NSCLC. The expression NSCLC was assessed using TCGA database, western blotting, immunohistochemistry. Correlations clinicopathological survival features were evaluated. functions regulating...
Abstract Mps one binder 2 (MOB2) regulates the NDR kinase family, however, whether and how it is implicated in cancer remain unknown. Here we show that MOB2 functions as a tumor suppressor glioblastoma (GBM). Analysis of expression glioma patient specimens bioinformatic analyses public datasets revealed was downregulated at both mRNA protein levels GBM. Ectopic suppressed, while depletion enhanced, malignant phenotypes GBM cells, such clonogenic growth, anoikis resistance, formation focal...
A number of new cell death processes have been discovered in recent years, including ferroptosis, which is characterized by the accumulation lipid peroxidation products derived from iron metabolism. The evidence suggests that ferroptosis has a tumor-suppressor function. However, mechanism mediates response tumor cells to oncolytic viruses remains poorly understood. Newcastle disease virus (NDV) can selectively replicate cells. We show NDV-induced acts through p53-SLC7A11-GPX4 pathway....
Epidermal growth factor (EGF) induces paxillin tyrosine dephosphorylation and Src activation, but the signaling pathways that mediate these responses were largely undefined. We found Gab1, a docking protein for SHP2 protein-tyrosine phosphatase in EGF-stimulated cells, was associated with paxillin. dephosphorylated caused dissociation of Csk, negative regulator Src, from had no effect on paxillin-Src association. A lower level Tyr-530 phosphorylation detected paxillin-associated cells....
Gab1-SHP2 association is required for Erk mitogen-activated protein kinase activation by several growth factors. interaction activates SHP2. However, an activated SHP2 still needs to associate with Gab1 mediate activation. It was unclear whether dephosphorylate a negative phosphorylation site on or the pleckstrin homology (PH) domain target it plasma membrane. We found that expression of fusion consisting PH and active (Gab1PH-SHP2DeltaN) induced constitutive Mek1 Erk2 Linking SHP2DeltaN...
Gamabufotalin (CS-6), a major bufadienolide of Chansu, has been used for cancer therapy due to its desirable metabolic stability and less adverse effect. However, the underlying mechanism CS-6 involved in anti-tumor activity remains poorly understood.The biological functions gamabufotalin (CS-6) were investigated by migration, colony formation apoptosis assays NSCLC cells. The nuclear localization interaction between transcriptional co-activator p300 NF-κB p50/p65 their binding COX-2...
Abstract Aberrant splicing is frequently found in cancer, yet the biological consequences of such alterations are mostly undefined. Here we report that Hippo–YAP signalling, a key pathway regulates cell proliferation and organ size, under control switch. We show TEAD4, transcription factor mediates undergoes alternative facilitated by tumour suppressor RBM4, producing truncated isoform, TEAD4-S, which lacks an N-terminal DNA-binding domain, but maintains YAP interaction domain. TEAD4-S...
Ferroptosis is a new kind of regulated cell death that characterized by highly iron-dependent lipid peroxidation. Cancer cells differ in their sensitivity to ferroptosis. Here we showed the Suppressor fused homolog (SUFU), critical component Hedgehog signaling, regulates ferroptosis breast cancer cells. Ectopic SUFU expression suppressed, whereas depletion enhanced RSL3-triggered through deregulation ACSL4. Moreover, promoted activation Yes-associated protein (YAP), thereby increasing...
Abstract Androgen receptor plays a critical role in the development of primary as well advanced hormone-refractory prostate cancer. Therefore, ablation androgen from cancer cells is an interesting concept for developing new therapy not only androgen-dependent but also metastatic cancer, which there no effective treatment available. We report here that LAQ824, cinnamyl hydroxamatic acid histone deacetylase inhibitor currently human clinical trials, effectively depleted at nanomolar...
The Yes-associated protein (YAP), a transcriptional coactivator inactivated by the Hippo tumor suppressor pathway, functions as an oncoprotein in variety of cancers. However, its contribution to breast cancer remains controversial. This study investigated role YAP cells under nutrient deprivation (ND). Here, we show that knockdown sensitized MCF7 deprivation-induced apoptosis. Furthermore, response ND, increased autolysosome degradation, thereby enhancing cellular autophagic flux cells. Of...
// Yao Xiao 1,* , Jingshu Wang 2,* Yu Qin 1 Yang Xuan Yunlu Jia 3 Wenxian Hu Wendan Meng Dai Zhenglin Li Canhui Yi Shilei Zhao Mei Sha Du Wei Cheng Xiangsheng 2 Yiming Chen Taihua Wu Songshu Yuhui Yuan Quentin Liu 1,2 Wenlin Huang 2,4 Guo Shusen and Wuguo Deng 1,2,4 Institute of Cancer Stem Cell & First Affiliated Hospital, Dalian Medical University, Dalian, China Sun Yat-sen University Center, State Key Laboratory Oncology in South China, Collaborative Innovation Center Medicine, Guangzhou,...
Background: Oncolytic viruses (OVs) are emerging as potent inducers of immunogenic cell death (ICD), releasing danger-associated molecular patterns (DAMPs) that induce anticancer immunity. Newcastle disease virus (NDV) has been shown to educe ICD in both glioma and lung cancer cells. The objective this study is investigate whether oncolytic NDV induces melanoma cells how it regulated. Methods: Various time points were actuated check the expression release markers induced by strain, NDV/FMW...
Three members of Gab family docking proteins, Gab1, Gab2 and Gab3, have been identified in humans. Previous studies found that the hepatocyte growth factor preferentially utilizes Gab1 for signalling, whereas Bcr-Abl selectively signals through Gab2. Gab1–SHP2 interaction has shown to mediate ERK (extracellular-signal-regulated kinase) activation by EGF (epidermal factor). However, it was unclear whether signalling is dispensable cells where are co-expressed. Using T47D MCF-7 human breast...
Oncolytic viruses represent a promising therapy against cancers with acquired drug resistance. However, low efficacy limits its clinical application. The objective of this study is to investigate whether pharmacologically modulating autophagy could enhance oncolytic Newcastle disease virus (NDV) strain NDV/FMW virotherapy drug-resistant lung cancer cells. effect infection on machinery in A549 cell lines resistant cisplatin (A549/DDP) or paclitaxel (A549/PTX) was investigated by detection...