A5049623814- Bacterial Infections and Vaccines
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- Peripheral Neuropathies and Disorders
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Immune responses and vaccinations
- IL-33, ST2, and ILC Pathways
- Vector-Borne Animal Diseases
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Protist diversity and phylogeny
- Immune cells in cancer
- Colorectal Cancer Treatments and Studies
- Cancer Immunotherapy and Biomarkers
- Genetic factors in colorectal cancer
- Transgenic Plants and Applications
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
La Jolla Institute for Immunology
2020-2025
Animal and Plant Health Agency
2016-2021
Discovery Institute
2018
Sanford Burnham Prebys Medical Discovery Institute
2018
Centre de Recerca en Sanitat Animal
2014
Universitat Autònoma de Barcelona
2014
Introduction Previous studies suggest that monocytes are an important contributor to tuberculosis (TB)-specific immune signatures in blood. Methods Here, we carried out comprehensive single-cell profiling of paired blood samples active TB (ATB) patients at diagnosis and mid-treatment, healthy controls. Results At diagnosis, ATB displayed increased monocyte-to-lymphocyte ratio, frequency CD14+CD16- intermediate CD14+CD16+ monocytes, upregulation interferon signaling genes significantly...
The immune response elicited by the protective whole-cell pertussis (wP) versus less-protective acellular (aP) vaccine has been well characterized; however, important clinical problems remain unsolved, as inability of currently administered aP is resulting in reemergence disease (i.e., whooping cough). Strong evidence shown that original, childhood and wP priming vaccines provide a long-lasting imprint on CD4+ T cells impacts immunity. However, vaccination might prevent but not infection,...
The increased incidence of whooping cough worldwide suggests that current vaccination against Bordetella pertussis infection has limitations in quality and duration protection. resurgence been linked to the introduction acellular vaccines (aP), which have an improved safety profile compared with previously used whole-cell (wP) vaccines. To determine immunological differences between aP wP priming infancy, we performed a systems approach immune response booster vaccination. Transcriptomic,...
Abstract Conventional dendritic cells (cDC) are professional antigen-presenting that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants recently pigs. Pigs an important biomedical model species a key source of animal protein; therefore further understanding their system will help underpin the development disease prevention strategies. To characterise cDC populations porcine blood, DC were...
The palatine tonsil is the portal of entry for food and air, continuously subjected to environmental challenges including pathogens which use pharynx as a primary site replication. In pigs, this includes viruses causing porcine respiratory reproductive syndrome, classical African swine fever; diseases have impacted pig production industry globally. Despite importance tonsils in host defence, little known regarding phenotype myeloid cells resident tonsil. Here, we characterised five cell...
Systems vaccinology studies have identified factors affecting individual vaccine responses, but comparing these findings is challenging due to varying study designs. To address this lack of reproducibility, we established a community resource for Bordetella pertussis booster responses and host annual contests predicting patients' vaccination outcomes. We report here on our experiences with the "dry-run" prediction contest. found that, among 20+ models adopted from literature, most successful...
Little is known about the host factor in response to PRRSV vaccination. For this purpose, piglets were immunized with a commercial PRRSV-live vaccine and classified as high responders (HR) or low (LR) regards frequencies of virus-specific IFN-γ-secreting cells. Six weeks post vaccination, PBMCs isolated from three individuals most extreme responses each HR LR groups 3 unvaccinated controls, either stimulated phytohaemagglutinin, challenged mock treated for 24 h, prior conducting...
<h3>Background</h3> Autologous chimeric antigen receptor (CAR) T cell therapies have revolutionized the treatment of some cancers and are now demonstrating effects in autoimmune disease. With several approved on market cure rates approaching 50% various hematologic malignancies, access to these life-saving is paramount. However, challenges with manufacturing, scaling, need for inpatient necessitate a truly off-the-shelf solution, especially non-oncology indications. Utilizing success mRNA...
Outbreaks of
Classical swine fever (CSF) is a highly contagious disease caused by the classical virus (CSFV). The live attenuated C-strain vaccine efficacious, initiating protection within several days of delivery. strain detected in tonsil early after inoculation, yet little known role that tonsillar immune cells might play protection. Comparing with pathogenic CSFV Alfort-187 strain, changes myeloid cell compartment were observed. infection led to emergence an additional CD163+CD14+ population, which...
Computational models that predict an individual's response to a vaccine offer the potential for mechanistic insights and personalized vaccination strategies. These are increasingly derived from systems vaccinology studies generate immune profiles human cohorts pre- post-vaccination. Most of these involve relatively small profile single vaccine. The ability assess performance resulting would be improved by comparing their on independent datasets, as has been done with great success in other...
Abstract Whole-cell inactivated vaccine against Bordetella pertussis (wP) was substituted in many countries by an acellular subunit (aP) to reduce side effects. Recent epidemiological studies have shown that aP vaccination infancy induces less durable immunity than wP vaccination. To determine immunological differences associated with vs. priming, we performed system-level profiling of the immune response adults primed following Tdap booster as a surrogate antigen encounter vivo . Shared...
Abstract Whooping cough is a contagious disease caused by Bordetella Pertussis. The introduction of an inactivated whole bacteria vaccine (wP) in the 1940s led to dramatic decrease cases. However, due related side-effects, this was substituted acellular (aP) 90’s which resulted rise In order determine if there are differences between those primed with aP vs. wP infancy, we created model using longitudinal samples from boosted individuals as proxy for antigen encounter vivo. previous...