A5103065031- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immune cells in cancer
- MicroRNA in disease regulation
- Glioma Diagnosis and Treatment
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Neuropeptides and Animal Physiology
- Cancer, Stress, Anesthesia, and Immune Response
- Nanoplatforms for cancer theranostics
- Infrared Thermography in Medicine
- Cancer Mechanisms and Therapy
- Neuroscience and Neuropharmacology Research
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Salivary Gland Disorders and Functions
- interferon and immune responses
- Antimicrobial Resistance in Staphylococcus
- Brain Metastases and Treatment
- Extracellular vesicles in disease
- Immune Response and Inflammation
Lanzhou University
2025
The University of Texas MD Anderson Cancer Center
2014-2024
Shandong University of Traditional Chinese Medicine
2024
Changsha Normal University
2022
Hunan Normal University
2022
Xiamen University
2017-2022
Baylor College of Medicine
2020
Xiamen Maternal and Child Health Hospital
2019
The University of Texas at Austin
2013
Jilin University
2012
Glioblastoma is highly enriched with macrophages, and osteopontin (OPN) expression levels correlate glioma grade the degree of macrophage infiltration; thus, we studied whether OPN plays a crucial role in immune modulation. Quantitative PCR, immunoblotting, ELISA were used to determine expression. Knockdown was achieved using complementary siRNA, shRNA, CRISPR/Cas9 techniques, followed by series vitro functional migration immunological assays. gene-deficient mice examine roles...
The progressive inflammatory process found in transforming growth factor beta1 (TGF-beta1)-deficient mice is associated with several manifestations of autoimmunity, including circulating antibodies to nuclear antigens, immune complex deposition, and increased expression both class I II major histocompatibility (MHC) antigens. contribution MHC antigens the genesis this phenotype has been determined by crossing TGF-beta1-null [TGF-beta1(-/-)] genotype into II-deficient [MHC-II(-/-)]...
Abstract Purpose: The signal transducer and activator of transcription 3 (STAT3) is frequently overexpressed in most cancers, propagates tumorigenesis, a key regulator immune suppression cancer patients. We sought to determine the incidence phosphorylated STAT3 (p-STAT3) expression malignant gliomas different pathologic types, whether p-STAT3 negative prognostic factor, influences inflammatory response within gliomas. Methods: Using immunohistochemical analysis, we measured 129 patients with...
Mice bearing the TGF-beta 1 null mutation (-/-) develop lymphoid infiltrates in heart, lungs, salivary glands, and other organs similar to those seen pseudolymphoma of Sjögren's Syndrome. We studied sera from -/- mice found elevated Ab levels dsDNA, ssDNA, Sm ribonucleoprotein. No Abs SSA/Ro or SSB/La no IgM rheumatoid factor were found. Serum autoantibodies predominately IgG specific as shown by ELISA inhibition studies. Antinuclear patterns on Western blots varied one mouse next,...
Abstract Purpose: Anti-programmed cell death protein 1 (PD-1) therapy has demonstrated inconsistent therapeutic results in patients with glioblastoma (GBM) including those profound impairments CD8 T-cell effector responses. Experimental Design: We ablated the CD8α gene BL6 mice and intercrossed them Ntv-a to determine how T cells affect malignant progression forming endogenous gliomas. Tumor-bearing were treated PD-1 efficacy of this treatment absence cells. The tumor microenvironment...
Gram-negative bacterial infection is a common cause of septic shock in the older population U.S. We employed an experimental model sepsis to study increased lethality due LPS animals. Three ages male B6JC3J/Nia mice, young (2 mo old), mature (12 and senescent (24 were treated with LPS, mice found be 10 times more sensitive lethality. Increased sensitivity correlated significantly elevated plasma TNF-alpha nitric oxide levels. Abs afforded aged animals passive protection against supralethal...
Significance The bacterial cell wall peptidoglycan is essential for maintenance of viability and yet dynamic, permitting growth division. Peptidoglycan synthesis inhibited by important antibiotics, including β-lactams vancomycin. Using the human pathogen Staphylococcus aureus , we have examined homeostatic mechanisms how their interruption leads to death. This has revealed two antibiotic-induced killing mediated specific hydrolases, both involving appearance holes that span entire thickness...
Abstract Although tissue-resident memory T (T RM ) cells specific for previously encountered pathogens have been characterized, the induction and recruitment of brain following immune therapy has not observed in context glioblastoma. Here, we show that expressing fibrinogen-like 2 (FGL2)–specific single-chain variable fragments (T-αFGL2) can induce tumor-specific CD8 + prevent glioblastoma recurrence. These display a highly expanded cell receptor repertoire distinct from found peripheral...
Studies have indicated that soluble products, including chemotactic factors, released by activated lung macrophages and fibroblasts are critical mediators in the pathogenesis of asbestos-induced pulmonary fibrosis. We provide evidence produced epithelial cells response to asbestos may also contribute disease. In present study, carcinogenic fibrogenic fibers, chrysotile crocidolite asbestos, were shown directly stimulate human type-II cell line, A549, a lesser degree primary bronchial cells,...
Two-component systems (TCSs) are key elements in bacterial signal transduction response to environmental stresses. TCSs generally consist of sensor histidine kinases (SKs) and their cognate regulators (RRs). Many SKs exhibit autokinase, phosphoryltransferase phosphatase activities, which regulate RR activity through a phosphorylation dephosphorylation cycle. However, how perform different enzymatic activities is poorly understood. Here, several crystal structures the minimal catalytic region...
Dynorphins (dyn) are a major class of endogenous opioid peptides that modulate the functions immune cells. However, effects dyn on glial cells in central nervous system (CNS) have not been well characterized. Because nitric oxide (NO) and proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) produced by involved various physiopathological conditions CNS, this study examined production NO TNF-alpha from mouse treated with lipopolysaccharide (LPS). LPS induced...
Abstract Melanoma is a common and deadly tumor that upon metastasis to the central nervous system (CNS) has median survival duration of less than 5 months. Activation signal transducer activator transcription 3 (STAT3) been identified as key mediator drives fundamental components melanoma. We hypothesized WP1066, novel inhibitor STAT3 signaling, would enhance antitumor activity cyclophosphamide (CTX) against melanoma, including disease within CNS. The mechanisms efficacy were investigated by...
Diabetes and periodontitis exhibit a bidirectional relationship, posing significant challenges for the treatment of in patients with diabetes. Our previous studies showed that hypoglycemic agent liraglutide (LIRA), together glycemic control, had favorable therapeutic effects on diabetic (DP), achieving "two birds one stone" effect. Therefore, exploration topical application LIRA treating DP is warranted. In this study, nanoparticles were loaded using poly (lactic-co-glycolic acid) (PLGA),...
Mice homozygous for a disrupted TGF-beta1 allele develop multiple lymphoproliferative disorders similar to those seen in the pseudolymphoma of Sjögren's syndrome. At 2 wk age, these mutant mice begin wasting syndrome and die at around 4 5 age. We studied salivary glands from symptomatic >14 days Reverse transcriptase-PCR analysis showed up-regulation proinflammatory cytokine genes such as IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, IL-10, IFN-gamma mice. Enhanced expression intercellular adhesion...
The signal transducer and activator of transcription 3 (STAT3) is a key molecular hub tumorigenesis immune suppression. expression phosphorylated STAT3 (p-STAT3) has been shown to be higher in melanoma metastasis the central nervous system (CNS) relative distant rest body (systemic). We sought determine whether increased p-STAT3 non-CNS systemic associated with an risk developing CNS negative prognostic factor for overall survival time.We retrospectively identified 299 patients stage IV...
The natural hormone estradiol (E2) induces tumors in rodents and various types of DNA damage vitro vivo, but has not been mutagenic bacterial or mammalian assays. Recent reports chromosomal genetic lesions induced by E2 led us to re-examine the mutation frequency molecular alterations hypoxanthine-guanine phosphoribosyltransferase (hprt) gene Chinese hamster V79 cells. at both physiological pharmacological concentrations (10-11, 10-10, 10-7, 10-6 M) significantly increased hprt 2. 57-,...