A5090984428- Immunotherapy and Immune Responses
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- interferon and immune responses
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Peptidase Inhibition and Analysis
- Biofuel production and bioconversion
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Synthesis and Characterization of Heterocyclic Compounds
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Heparin-Induced Thrombocytopenia and Thrombosis
- Immune responses and vaccinations
- Hepatitis B Virus Studies
- Algal biology and biofuel production
- Poxvirus research and outbreaks
- Effects and risks of endocrine disrupting chemicals
- Escherichia coli research studies
- Crystallization and Solubility Studies
- Bacterial biofilms and quorum sensing
- Molecular Communication and Nanonetworks
- Diagnosis and treatment of tuberculosis
Shaanxi University of Science and Technology
2025
University of British Columbia
2025
Guizhou University
2019-2022
The Affiliated Hospital to Changchun University of Chinese Medicine
2021
University of Nottingham
2008
Nottingham City Hospital
2008
University of Cambridge
2007
Wellcome Trust
2007
Imperial College London
2007
Hammersmith Hospital
1995-2006
The 38-kDa glycolipoprotein of Mycobacterium tuberculosis has been known to evoke prominent T cell and Ab responses in patients with active tuberculosis. In this study, we investigated its protective capacity using plasmid DNA immunization a mouse experimental model. Prior knowledge several antigenic determinants beneficial for analyzing the phenotype specificity cells, which determine efficacy vaccination procedure. C57BL/6 mice responded gene-pcDNA3 strong CD4+ Th1 CD8+ cytotoxic...
Immunization of mice with a recombinant vaccinia virus expressing the human papillomavirus type 16 (HPV-16) E6 gene elicits specific antibody, proliferative and cytotoxic T lymphocyte responses. B cell epitopes were mapped by using synthetic peptides. This study provides background to future investigation aimed at developing prophylactic therapeutic vaccines against HPV-16 infection cervical cancer.
Abstract The central importance of dendritic cells (DC) in both innate and acquired immunity is well recognized the mammalian immune system. By contrast DC have yet to be characterized avian species despite fact that such as chicken a well-developed CD83 has proven an excellent marker for human murine systems. In this study we identify (chCD83) equivalent CD83. We demonstrate first time unlike CD83, chCD83 uniquely expressed B cell areas secondary lymphoid organs with no or bursa Harderian...
The purpose of this study was to explore the molecular mechanism Danggui Buxue Decoction (DBD) intervening premature ovarian failure (POF).The active compounds-targets network, compounds-POF-targets and protein-protein interaction (PPI) network were constructed by a pharmacology approach: Gene Ontology (GO) function Kyoto Encyclopedia Genome (KEGG) pathway analysis DAVID 6.8 database. docking method used verify between core components DBD targets. Then, High-Performance Liquid Chromatography...
A number of subunit-based vaccine candidates have recently begun to erode the exclusive position Mycobacterium bovis bacillus Calmette-Guérin (BCG), which gives unpredictable and highly variable protection against tuberculosis. In this paper we investigated protective capacity 19,000 MW 38,000 glyco-lipoproteins M. tuberculosis expressed by recombinant vaccinia viruses in a mouse infection model. Both proteins were at high levels vaccinia-infected cells. addition, two inoculations C57B1/6...
Dysfunctional dendritic cells (DC) are common in cancer patients; however, the underlying molecular targets poorly understood. Nevertheless, adoptive-transfer and situ vaccination protocols continue, largely without addressing immune-suppression. Understanding tumour-mediated DC suppression would assist rational design of next generation immunotherapy. This study used a tumour-lysate loaded model adoptive immunotherapy also necrotic many tumours. Patient-derived healthy-donor...
Multinucleated giant cells (MGCs) are characteristic of granulomatous inflammation. Matrix metalloproteinase (MMP)- 9, the major monocyte-derived matrix metalloproteinase, is key in inflammatory tissue damage. At 72 h, MGCs secrete 153 ± 2.5 ng/mL MMP-9, compared with 115 3.8 during macrophage differentiation (P < .05). In contrast, level MGC secretion-specific inhibitor, inhibitor (TIMP)-1, lower Mature constitutively greater concentrations MMP-9 than do monocytes or macrophages tuberculous...
Intravesical administration of live M. bovis BCG organisms for carcinoma in situ the urinary bladder is most successful immunotherapy solid malignancy. Nevertheless BCG-therapy associated with significant toxicity and ineffective 30-40% cases. Recently it has been proposed that cytokine-modified mycobacteria may give greater efficacy. As any involving (wild-type or recombinant) likely to have (notably immunocompromised), we examined anti-tumour potential closely related nonpathogenic...
The transforming protein E7 of human papilloma virus type 16 can stimulate cytotoxic T lymphocytes (CTL) which protect experimental animals against growth expressing tumour cells. In this study we compared CTL responses in mice immunized with either MF59 adjuvant or recombinant vaccinia (Vac‐E7). We have chosen H‐2 d because no E7‐specific been described MHC haplotype. Immunization these Vac‐E7 generated lysed target cells infected transfected the gene. from showed specificity for same...
CD8+ T cells have been implicated in protective anti-tuberculous immune responses, but little is known about the identity of mycobacterial antigens recognized by cells. In this study we identified Mycobacterium tuberculosis 38 kDa protein as a target for murine cytotoxic lymphocytes (CTL) which were induced vaccination C57BL/6 mice with DNA delivered plasmid, transfected tumour or infection tubercle bacilli. Using overlapping synthetic peptides covering whole sequence, predicted to contain...
Mycobacterium tuberculosis is the single, most important cause of morbidity attributable to a single infectious organism. CD8+ T cells play an role in anti-tuberculous immune responses both mice and humans. Data concerning identity mycobacterial antigens recognized by limited; consequently, few CTL epitopes have been characterized. The authors identified allele-specific (H-2b d) MHC class I binding motifs six prominent M. protein (the 19 38 kDa lipoglycoproteins 10, 16, 65 70 stress...
Bufei decoction (BFD) has been applied to treat chronic obstructive pulmonary disease (COPD) for centuries as a recognized traditional Chinese herbal formula. However, mechanisms of BFD on COPD are unclear. This study conducts an inquiry into the underlying therapeutic effect COPD. A rat model with qi deficiency in lungs was established through induction using cigarette and sawdust smoking combined intratracheal instillation lipopolysaccharide following treatment 28 days. Changes Th17/Treg...
Position-dependent gene expression is a critical aspect of the development and behaviour multicellular organisms. It requires complex series interactions to occur between different cell types in addition intracellular signalling cascades. We used Escherichia coli study properties an artificial system at interface two expanding populations. genetically engineered one population produce diffusible acyl-homoserine lactone (AHL) signal, another respond it. Our experiments demonstrate how such...
Caprine parainfluenza virus type 3 (CPIV3), a new strain of virus, was isolated from the goats in 2014 China. Studies have shown that viral infection can induce changes expression profile host miRNAs, which modulate natural immune responses and infection. In this study, we report bta-miR-677 suppressed CPIV3 replication Madin-Darby bovine kidney (MDBK) cells guinea pigs. Bta-miR-677 overexpression promoted I interferon (IFN-I) IFN-stimulated genes (ISGs) production, thereby inhibiting...
Porcine deltacoronavirus (PDCoV) is an emenging swine enteropathogenic coronavirus that can cause high mortality rate. Little known regarding the pathogenicity of PDCoV against 27-years-old piglets. In this study, piglets were experimentally infected with from cell culture, challenged do not have obvious symptoms 1 to 7 days post-challenge (DPC), while viral shedding was detected in rectal swab at DPC. Tissues small intestines displayed slight macroscopic and microscopic lesions no antigen...