A5029590918- Mesenchymal stem cell research
- Biomedical Ethics and Regulation
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Periodontal Regeneration and Treatments
- Virus-based gene therapy research
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Bone fractures and treatments
- Hematopoietic Stem Cell Transplantation
- RNA Interference and Gene Delivery
- Orthopaedic implants and arthroplasty
- Bone and Joint Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Cells and Metastasis
- Cell death mechanisms and regulation
- Cytomegalovirus and herpesvirus research
- Knee injuries and reconstruction techniques
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Extracellular vesicles in disease
- Spinal Cord Injury Research
- Abdominal vascular conditions and treatments
Banc de Sang i Teixits
2015-2024
Universitat Autònoma de Barcelona
2015-2024
Vall d'Hebron Institut de Recerca
2017-2024
Instituto de Salud Carlos III
2023
Deleted Institution
2023
Institute of Research and Innovation Parc Tauli
2021
Corporació Sanitària Parc Taulí
2020-2021
Weatherford College
2020
University of Almería
2010-2012
Wellcome/MRC Cambridge Stem Cell Institute
2011
Abstract We have generated embryonic stem (ES) cells and transgenic mice with green fluorescent protein (GFP) inserted into the Pitx3 locus via homologous recombination. In central nervous system, ‐directed GFP was visualized in dopaminergic (DA) neurons substantia nigra ventral tegmental area. Live primary DA can be isolated by fluorescence‐activated cell sorting from these mouse embryos. culture, –GFP is coexpressed a proportion of ES‐derived neurons. Furthermore, ES cell‐derived...
Contrary to the minimal criteria proposed by International Society for Cell and Gene Therapy defining multipotent mesenchymal stromal cells (MSC), human leukocyte antigen (HLA)-DR expression is largely unpredictable in ex vivo-expanded clinical-grade cultures. Although activation of MSC culture does not appear affect their functionality, a large study investigating impact HLA-DR on cell identity potency still missing literature. A retrospective analysis 130 clinical batches bone marrow...
Cell and gene therapy poses evolving challenges. The current article summarizes the discussions held by European Regional Committee of International Society for & Gene Therapy Blood Marrow Transplantation (EBMT) on challenges in this field, focusing setting. This emphasizes imperative assessment real-world cell activity, advocating expanded registries beyond hematopoietic transplantation chimeric antigen receptor-T-cell therapy. Accreditation's role ensuring standardized procedures, as...
<h2>Abstract</h2><h3>Background</h3> Small cardiac tissue engineering constructs show promise for limiting post-infarct sequelae in animal models. This study sought to scale-up a 2-cm<sup>2</sup> preclinical construct into human-size advanced therapy medicinal product (ATMP; PeriCord), and test it first-in-human implantation. <h3>Methods</h3> The PeriCord is clinical-size (12–16 cm<sup>2</sup>) decellularised pericardial matrix colonised with human viable Wharton's jelly-derived mesenchymal...
Background: The selection of assays suitable for testing the potency clinical grade multipotent mesenchymal stromal cell (MSC)-based products and its interpretation is a challenge both developers regulators. Here, we present bioprocess design production Wharton’s jelly (WJ)-derived MSCs validated immunopotency assay approved by competent regulatory authority batch release together with study failure modes in potential impact on critical quality attributes (CQA) final product. Methods:...
In this study, data are presented on serologic H-2 typing of 320 wild mice collected in several parts Europe and Egypt. The sample was typed for 39 class I (K, D) 16 II (Ia) antigens. phenotype frequencies antigens showed high variability their distribution among different areas, ranging from absence or presence low frequency one area to about one-half the another area. average phenotypic private 6.3%; at least 60% alleles (blanks) could not be identified with available reagents. suggest...
The availability of specific markers expressed in different regions the developing nervous system provides a useful tool to illuminate their development, regulation and function. We have identified by expression profiling putative non-coding RNA, Rmst, that exhibits prominent midbrain floor plate region, isthmus roof anterior neural tube. At developmental stage when ventral dopaminergic neuron territory is being established, Rmst appears be restricted presumptive neurons tegmental area lies...
Abstract Background Orthopaedic diseases are one of the major targets for regenerative medicine. In this context, Wharton’s jelly (WJ) is an alternative source to bone marrow (BM) allogeneic transplantation since its isolation does not require invasive procedure cell collection and raise ethical concerns. However, osteogenic capacity human WJ-derived multipotent mesenchymal stromal cells (MSC) remains unclear. Methods Here, we compared baseline potential MSC from WJ BM sources by cytological...
Multipotent mesenchymal stromal cells (MSCs) are currently the most extensively studied type of adult stem in advanced stages development field regenerative medicine. The biological properties MSCs have generated great hope for their therapeutic use degenerative and autoimmune conditions that, at present, lack effective treatment options. Over last decades, been typically obtained from bone marrow, but extraction process is highly invasive quality numbers isolated drastically influenced by...