High-risk human papillomaviruses (HPVs) encode two viral oncoproteins, E6 and E7, from a single bicistronic pre-mRNA containing three exons introns. Retention of intron 1 in the coding region is essential for production full-length oncoprotein. However, splicing extremely efficient cervical cancer cells, leading to spliced transcript, E6*I, E6. Here, we investigated whether this might benefit E7 production. Using RNA interference as tool, targeted using small interfering RNAs (siRNAs)...

Paeonol, as one of the most abundant plant-derived polyphenols, has multiple bioactivities including anti-inflammatory, anti-tumor, and anti-cardiovascular diseases. Nevertheless, anti-aging effects related mechanisms paeonol are rarely reported. In this study, we found that significantly prolonged mean lifespan Caenorhabditis elegans (C. elegans) by 28.49% at a dose 200 μM. Moreover, promoted health C. increasing body bending pharyngeal pumping rates reducing lipofuscin accumulation level....

The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the via interactions with A/C-rich RNA elements. An SE4 element regulates selection bovine type 1 (BPV-1) late-specific splice site, binding SRp20 suppresses this selection. Expression late BPV-1 L1 or human (HPV) L1, major capsid protein, inversely correlates levels...

Polyphenol oxidase (PPO) causes the browning reaction in fruits and vegetables deteriorates quality. Thermal treatment for enzyme inactivation may result defects as opposed to high pressure CO₂ (HPCD) processing. In this study, changes activity, dissociation, aggregation conformation of purified PPO from thermal HPCD treated juice were investigated. exhibited at 55⁻65 °C whereas processing alone same temperature resulted still showing activity. Under 25 65 °C, degree was higher (0.39 0.24)...

Mung beans possess health benefits related to their bioactive ingredients, mainly flavonoids, which are highly concentrated in the coat. However, anti-aging effects of mung rarely reported. In this work, we found that bean coat extract (MBCE), rich vitexin and isovitexin, extended lifespan promoted Caenorhabditis elegans (C. elegans) without any disadvantages. Moreover, MBCE enhanced resistance heat oxidation C. by reducing accumulation intracellular reactive oxygen species up-regulating...

Caffeoylquinic acids, as plant-derived polyphenols, exhibit multiple biological activities such antioxidant, anti-inflammatory, and neuroprotective activities. However, only limited information about their effect on longevity is available. In the current study, molecular docking was employed to explore interactions between six representative caffeoylquinic acids insulin-like growth factor-1 receptor (IGFR), which an important target protein for longevity. The results indicated that all...

Abstract BACKGROUND Artemisia selengensis Turcz. (AST) is a common edible and medicinal herb possessing extensive biological activities various health‐promoting functions. However, the anti‐aging effects of AST have been neglected. This work evaluated leaf extract (ASTE) on stress tolerance longevity in Caenorhabditis elegans . RESULTS ASTE treatment enhanced resistance significantly extended lifespan C. Moreover, prolonged healthspan by increasing body bending pharyngeal pumping rates,...

Scope Vitexin and isovitexin are natural plant nutraceuticals for human health longevity. This research investigates the underlying mechanisms of vitexin on aging health. The vital role DAF‐2/insulin‐like growth factor‐1 receptor (IGFR) is illustrated in insulin/insulin‐like signaling pathway (IIS) modulated by isovitexin. Methods results In vitro, vivo models molecular docking methods performed to explore antiaging mechanism (50 100 µM) extended lifespan Caenorhabditis elegans . declines...

Edible chrysanthemum is a common food resource for tea and functional foods with potential benefits human health. Studies have indicated that has the effect on inflammatory diseases, while effects gouty inflammation remain underexplored. The present study aimed to investigate anti-gout activity characterize active ingredients of chrysanthemums by using metabolite profiles, in vitro experiments, spectrum-effect analysis. Results showed 'Boju' (BJ), 'Hangbaiju' (HBJ), 'Huaiju' (HJ) exhibited...

Abstract Background: Breast cancer (BC), the most common globally and a leading cause of death in women, often utilizes radiation therapy (RT) as part treatment, with over 85% patients receiving RT after breast-conserving surgery. Still, 15% women experience local recurrence despite RT, underscoring need to better understand molecular mechanisms driving response resistance. We hypothesized that transcriptomic changes occur ionizing intrinsically radiosensitive resistant BC models would offer...

Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization transformation of infected keratinocytes epithelial cells. Although it unclear how 16E6 disrupts the epigenetic profile host genes, its presence in nucleus a key feature. The present report describes intrinsic properties influence import living When coding region full-length was inserted frame into C...

Ginger is a traditional medicinal and edible plant with multiple health-promoting properties.

Bovine papillomavirus type 1 (BPV-1) late pre-mRNAs are spliced in keratinocytes a differentiation-specific manner: the leader 5' splice site alternatively splices to proximal 3' (at nucleotide 3225) express L2 or distal 3605) L1. Two exonic splicing enhancers, each containing two ASF/SF2 (alternative factor/splicing factor 2) binding sites, located between sites and have been identified as regulating alternative usage. The present report demonstrates for first time that is required under...

Diabetes mellitus is well known to cause bladder dysfunction; however, the molecular mechanisms governing this process and effects on individual tissue elements within are poorly understood, particularly in type 2 diabetes. A shotgun proteomics approach was applied identify proteins differentially expressed between diabetic (TallyHo) control (SWR/J) mice smooth muscle urothelium, separately. We were able 1760 nonredundant from detrusor 3169 urothelium. Pathway network analysis of...

Alcoholic liver disease (ALD) is still a global health concern. Long-term alcohol intake alters the gut microbiota diversity and metabolic activity, causes intestinal barrier dysfunction, leading to development of ALD. This research explored protective effects underlying mechanisms red raspberry (RR) on alcohol-related disorders in mice. Male C57BL/6J mice were fed standard diet or supplemented with 2%, 4%, 8% weight/weight RR. Meanwhile, administered 35% (v/v) ethanol (EtOH, 10 mL per kg...

Animal models of vaginal distention (VD) have demonstrated increased expression chemokine (C-C motif) ligand 7 (CCL7) In this study, we investigated the CCL7 in mice simulated birth trauma-induced urinary incontinence using VD and pudendal nerve transection (PNT).Forty-nine were divided into 6 groups: VD, sham PNT, anesthesia, age-matched controls. The urethra, vagina, rectum harvested for immediately or 24 hours after assigned procedure. Venous sampling quantification serum was also...

Vitexin and isovitexin, as potential SKN-1/Nrf2 (SKN-1 is a homologous protein of mammalian Nrf2) activators, extended lifespan promoted healthspan in Caenorhabditis elegans. This study aims to elucidate the role vitexin isovitexin-induced anti-aging stress-resistance. isovitexin upregulated antioxidant gene expressions, reduced ROS accumulation, increased SKN-1 accumulation nucleus. They prolonged clear during stressful conditions skn-1-dependent manner. skn-1 was also found be necessary...

Diabetes affects the erectile function significantly. However, penile alterations in early stage of diabetes experimental animal models have not been well studied. We examined changes penis and its main components diabetic rats.Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics age-matched controls. Three or nine weeks after induction, was removed for immunohistochemical staining smooth muscle neuronal nitric oxide synthase (nNOS) midshaft tissues....