A5024506446- Pancreatic function and diabetes
- Pancreatitis Pathology and Treatment
- Pancreatic and Hepatic Oncology Research
- Diabetes Management and Research
- Diabetes and associated disorders
- Cannabis and Cannabinoid Research
- 3D Printing in Biomedical Research
- Trace Elements in Health
- Diabetes Treatment and Management
- Immunotherapy and Immune Responses
- Renal Transplantation Outcomes and Treatments
- Virus-based gene therapy research
- Organ and Tissue Transplantation Research
- Zebrafish Biomedical Research Applications
- Pediatric Hepatobiliary Diseases and Treatments
- Congenital heart defects research
- Gastrointestinal disorders and treatments
- Electrospun Nanofibers in Biomedical Applications
- Genetics and Neurodevelopmental Disorders
- Xenotransplantation and immune response
- Angiogenesis and VEGF in Cancer
- Sphingolipid Metabolism and Signaling
- T-cell and B-cell Immunology
- Metabolism and Genetic Disorders
- Heavy Metal Exposure and Toxicity
Royal Adelaide Hospital
2014-2024
The University of Adelaide
2013-2024
University of Wollongong
2023
Flinders Medical Centre
2017-2021
John Wiley & Sons (United States)
2020
Smith College
2020
Syracuse University
2020
University of Rochester
2020
University of South Australia
2018
Women's and Children's Hospital
2017
Whilst initial rates of insulin independence following islet transplantation are encouraging, long-term function using the Edmonton Protocol remains a concern. The aim this single-arm, multicenter study was to evaluate an immunosuppressive protocol antithymocyte globulin (ATG), tacrolimus and mycophenolate mofetil (MMF) followed by switching sirolimus MMF. Islets were cultured for 24 h prior transplantation. primary end-point HbA1c <7% cessation severe hypoglycemia. Seventeen recipients ≥ 12...
Abstract Over the last two decades, pancreatic islet transplantations have become a promising treatment for Type I diabetes. However, although providing consistent and sustained exogenous insulin supply, there are number of limitations hindering widespread application this approach. These include lack sufficient vasculature allogeneic immune attacks after transplantation, which both contribute to poor cell survival rates. Here, these issues addressed using biofabrication An...
Porous silicon nanoparticles (pSiNP), modified to target dendritic cells (DC), provide an alternate strategy for the delivery of immunosuppressive drugs. Here, we aimed develop a DC-targeting pSiNP displaying c-type lectin, cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), and CD11c monoclonal antibodies. The in vivo tracking these fluorescent was assessed both C57BL/6 mice common marmosets (Callithrix jacchus) by intravenous injection (20 mg/kg). Rapamycin...
Abstract Regulatory T‐cells (Tregs) are important modulators of the immune system through their intrinsic suppressive functions. Systemic adoptive transfer ex vivo expanded Tregs has been extensively investigated for allogeneic transplantation. Due to time‐consuming and costly expansion protocols Tregs, more targeted approaches could be beneficial. The encapsulation human natural induced localized immunosuppression is described first time. encapsulated in alginate‐gelatin methacryloyl...
Phenoxodiol (2H-1-benzopyran-7-0,1, 3-[4-hydroxyphenyl], PXD) is a synthetic analogue of the naturally-occurring plant isoflavone and anticancer agent, genistein. PXD directly induces mitotic arrest apoptosis in most cancer cells currently undergoing clinical trials, as chemotherapeutic ovarian prostate cancers. We show here that also exhibits potent antiangiogenic properties. Thus, it inhibited endothelial cell proliferation, migration capillary tube formation expression matrix...
Background: Failure to develop protective immunity in response vaccination is common among kidney transplant recipients, rendering them susceptible severe infection. Novel strategies are required. Here, we investigated the potential of mechanistic-target-of-rapamycin (mTOR) inhibitors improve vaccine responses. Methods: Humoral and cellular responses primary COVID-19 (ChAdOx1 or BNT162b2) were assessed for recipients receiving mTOR inhibitor-based (mTOR inhibitor, mycophenolate,...
The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve transplanted islets. In addition, EPCs directly affect β-cells. objective this study was to examine ability enhance murine syngeneic marginal mass transplant model mechanisms through which occurs. We found cotransplanted improved cure rate initial glycemic control Gene...
Intrahepatic islet transplantation for type 1 diabetes is limited by the need multiple infusions and poor viability posttransplantation. The development of alternative sites necessary to improve survival facilitate monitoring retrieval. We tested a clinically proven biodegradable temporizing matrix (BTM), polyurethane-based scaffold, generate well-vascularized intracutaneous “neodermis” within skin transplantation. In murine models, BTM did not impair syngeneic renal-subcapsular transplant...
AbstractPancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both survival transplanted islets. Here, we describe first time vitro formation rat mosaic pseudoislets comprised pancreatic β-cells with interspersed vasculogenic EPC. Bone marrow-derived EPC displayed a similar phenotype to non-adherent EPC, recently described human mouse. Mosaic pseudoislet...
Background The early loss of functional islet mass (50–70%) due to apoptosis after clinical transplantation contributes allograft failure. Insulin-like growth factor (IGF)-II is an antiapoptotic protein that highly expressed in β-cells during development but rapidly decreases postnatal life. Methods We used adenoviral (Ad) vector overexpress IGF-II isolated rat islets and investigated its action against exogenous cytokines interleukin-1β– interferon-γ–induced cell death vitro. Using...
Kidney transplant recipients (KTRs) are highly vulnerable to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and disease 2019 (COVID-19). The 28-day case mortality for KTRs is 24%, mounting evidence suggests poor immunogenicity clinical effectiveness of vaccines in this group.1Phanish M. Ster I.C. Ghazanfar A. et al.Systematic review meta-analysis COVID-19 kidney recipients, the South West London Transplant Network experience.Kidney Int Rep. 2021; 6: 574-585Google...
The initial response to islet transplantation and the subsequent acute inflammation is responsible for significant attrition of islets following both autologous allogenic procedures. This multicentre study compares this inflammatory using cytokine profiles complement activation.Inflammatory pathway activity were examined in two cohorts patients undergoing total pancreatectomy followed either by (n=11) or (n=6) transplantation. Two who underwent alone (n=2) served as controls.The peak...
Abstract Inadequate immune response to vaccination is a long-standing problem faced by immunosuppressed kidney transplant recipients (KTRs), requiring novel strategies improve vaccine efficacy. In this study, the potential of mechanistic target rapamycin inhibitors (mTORi) T cell responses COVID-19 was investigated. Following primary with adenoviral (ChAdOx1) or mRNA (BNT162b2) vaccines, KTRs receiving demonstrated greater than those healthy individuals, characterized increased frequencies...
Angiogenesis is a complex multicellular process requiring the orchestration of many events including migration, alignment, proliferation, lumen formation, remodeling, and maturation. Such complexity indicates that not only individual genes but also entire signaling pathways will be crucial in angiogenesis. To define an angiogenic blueprint regulated genes, we utilized our well-characterized three-dimensional collagen gel model vitro angiogenesis, which majority cells synchronously progress...
Abstract Porous biodegradable scaffolds have many applications in bioengineering, ranging from cell culture and transplantation, to support structures, induce blood vessel tissue formation vivo. While numerous strategies been developed for the manufacture of porous scaffolds, it remains challenging control spatial organization pores. In this study, we introduce use granular convection effect, also known as muesli or brazil nut rapidly engineer particulate templates with a vertical size...