A5011875361- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Neurobiology and Insect Physiology Research
- Insect Pest Control Strategies
- Forensic Entomology and Diptera Studies
- Insect behavior and control techniques
- Insect and Arachnid Ecology and Behavior
- Microbial Metabolic Engineering and Bioproduction
- Invertebrate Immune Response Mechanisms
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Circadian rhythm and melatonin
- Genetic diversity and population structure
- Developmental Biology and Gene Regulation
- Morphological variations and asymmetry
- CRISPR and Genetic Engineering
- Environmental Toxicology and Ecotoxicology
- Trace Elements in Health
- Cholinesterase and Neurodegenerative Diseases
- Nicotinic Acetylcholine Receptors Study
- Retinal Development and Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Physiological and biochemical adaptations
The University of Melbourne
2016-2025
Washington University in St. Louis
2023
The University of Queensland
2023
Biotechnology Institute
2006
La Trobe University
2003
Fred Hutch Cancer Center
2003
Cancer Research Center
2003
Commonwealth Scientific and Industrial Research Organisation
1998
Carnegie Mellon University
1996
U.S. National Science Foundation
1996
Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about origins and spread resistant alleles in global populations insects. Here, via microarray analysis all P450s Drosophila melanogaster , show that DDT-R a gene conferring to DDT, associated with overtranscription single cytochrome P450 gene, Cyp6g1 . Transgenic shows this alone both necessary sufficient for resistance. Resistance up-regulation are allele has...
Helicoverpa armigera and zea are major caterpillar pests of Old New World agriculture, respectively. Both, particularly H. armigera, extremely polyphagous, has developed resistance to many insecticides. Here we use comparative genomics, transcriptomics resequencing elucidate the genetic basis for their properties as pests.
The increased transcription of the Cyp6g1 gene Drosophila melanogaster, and consequent resistance to insecticides such as DDT, is a widely cited example adaptation mediated by cis-regulatory change. A fragment an Accord transposable element inserted upstream causally associated with has spread high frequencies in populations around world since 1940s. Here we report existence natural allelic series at this locus D. involving copy number variation Cyp6g1, two additional insertions (a P...
Cytochrome P450s form a large and diverse family of heme-containing proteins capable carrying out many different enzymatic reactions. In both mammals plants, some are known to carry reactions essential for processes such as hormone synthesis, while other involved in the detoxification environmental compounds. general, functions insect less well understood. We characterized Drosophila melanogaster P450 expression patterns embryos 2 stages third instar larvae. identified numerous expressed fat...
Abstract Background Arthropods comprise the largest and most diverse phylum on Earth play vital roles in nearly every ecosystem. Their diversity stems part from variations a conserved body plan, resulting recorded adaptive changes genome. Dissection of genomic record sequence change enables broad questions regarding genome evolution to be addressed, even across hyper-diverse taxa within arthropods. Results Using 76 whole sequences representing 21 orders spanning more than 500 million years...
Transposable elements are a major mutation source and powerful agents of adaptive change. Some transposable element insertions in genomes increase to high frequency because the selective advantage mutant phenotype provides. Cyp6g1-mediated insecticide resistance Drosophila melanogaster is due upregulation cytochrome P450 gene Cyp6g1, leading variety classes. The Cyp6g1 correlated with presence long terminal repeat (LTR) an Accord retrotransposon inserted 291bp upstream transcription start...
Helicoverpa armigera and H. zea are amongst the most significant polyphagous pest lepidopteran species in Old New Worlds respectively. Separation of is difficult usually only achieved through morphological differences genitalia. They capable interbreeding to produce fertile offspring. The single status has been doubted, due its wide distribution plant host range across World. This study explores global genetic diversity evolutionary relationship H zea.We obtained partial (511 bp)...
Declining insect population sizes are provoking grave concern around the world as insects play essential roles in food production and ecosystems. Environmental contamination by intense insecticide usage is consistently proposed a significant contributor, among other threats. Many studies have demonstrated impacts of low doses insecticides on behavior, but not elucidated links to insecticidal activity at molecular cellular levels. Here, histological, physiological, behavioral imidacloprid...
Global governance can be informed by a deliberative assembly composed of lay citizens
Lufenuron is an insect growth regulator insecticide mainly used for the control of cat flea. To understand mechanisms resistance to lufenuron, we have characterized lufenuron in a natural population Drosophila melanogaster . In this study precise genetic mapping identify mechanism resistance: overexpression cytochrome P450 gene Cyp12a4. Cyp12a4 predicted encode mitochondrial enzyme. Expression D. third-instar larvae was detected midgut and Malpighian tubules both lufenuron-resistant...
As classical phase II detoxification enzymes, glutathione S-transferases (GSTs) have been implicated in insecticide resistance and may evolved response to toxins the niche-defining feeding substrates of Drosophila species. We annotated GST genes 12 species with recently sequenced genomes analyzed their molecular evolution. Gene copy number variation is attributable mainly unequal crossing-over events large delta epsilon clusters. Within these gene clusters there are also slowly diverging...
We map 114 gene gains and 74 losses in the P450 family across phylogeny of 12 Drosophila species by examining congruence trees trees. Although number genes varies from to 94 examined, we infer that there were at least 77 ancestral genome. One most striking observations data set is elevated loss sechellia lineage. The gain events are not evenly distributed among genes—with 30 showing no or whereas others show as many 20 copy changes examined. clades fewest tend be those evolving with...
Abstract Lucilia cuprina is a parasitic fly of major economic importance worldwide. Larvae this invade their animal host, feed on tissues and excretions progressively cause severe skin disease (myiasis). Here we report the sequence annotation 458-megabase draft genome . Analyses 14,544 predicted protein-encoding genes provide unique insights into fly’s molecular biology, interactions with host insecticide resistance. These have broad implications for designing new methods prevention control myiasis.
The Resistance to Dieldrin gene, Rdl , encodes a GABA-gated chloride channel subunit that is targeted by cyclodiene and phenylpyrazole insecticides. gene was first characterized in Drosophila melanogaster genetic mapping of resistance the dieldrin. 4,000-fold observed due single amino acid replacement, Ala 301 Ser. equivalent change subsequently identified orthologs large range resistant insect species. Here, we report identification duplication at locus D. . 113-kb contains one WT copy...
Large-scale insecticide application is a primary weapon in the control of insect pests agriculture. However, growing body evidence indicates that it contributing to global decline population sizes many beneficial species. Spinosad emerged as an organic alternative synthetic insecticides and considered less harmful insects, yet its mode action remains unclear. Using
The Hsp90 chaperone buffers development against a wide range of morphological changes in many organisms and Drosophila masks the effects hidden genetic variation. Theory predicts that nongenetic buffering will share common mechanisms. For example, it is argued evolved solely as by-product environmental buffering, should mask deviations from any source. To test this idea, we examined effect on purely variation phenotype, measured differences between left right sides several bilaterally...