A5084191299- NMR spectroscopy and applications
- Advanced NMR Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced MRI Techniques and Applications
- Various Chemistry Research Topics
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Molecular spectroscopy and chirality
- Fish Ecology and Management Studies
- Biochemical effects in animals
- Nanopore and Nanochannel Transport Studies
- Experimental Learning in Engineering
- Microplastics and Plastic Pollution
- Innovative Teaching Methods
- Advanced Photocatalysis Techniques
- Epilepsy research and treatment
- Biomedical and Engineering Education
- Conducting polymers and applications
- Neurological Disease Mechanisms and Treatments
- Preterm Birth and Chorioamnionitis
- Amino Acid Enzymes and Metabolism
- Aquaculture disease management and microbiota
- Liver Disease Diagnosis and Treatment
Harvard University
2025
Massachusetts General Hospital
2024-2025
The Scarborough Hospital
2019-2024
University of Toronto
2019-2024
Toronto Public Health
2021
In U.S. Pacific Northwest coho salmon (
Used cooking oil disposal is a concern at the global scale. Direct from domestic households and restaurants via drains can result in serious environmental issues. In this study, waste was collected directly vats McDonald's restaurant acrylated straightforward one-step reaction. After addition of photoinitiator without any photoinhibitor, product be printed using commercial 3D printer. The formulated resin produced high-resolution prints with features down to 100 micrometers. rapid prototyped...
Abstract NMR/MRI are critical tools for studying molecular structure and interactions but suffer from relatively low sensitivity spectral overlap. Here, a Nuclear Magnetic Resonance (NMR) approach, termed DREAMTIME, is introduced that provides “a window” inside complex systems, capable of showing only what the user desires, with complete specificity. The chooses list molecules interest, approach detects those targets while all other invisible. demonstrated in whole human blood urine, small...
Photocatalysis is typically monitored via analysis of phases in isolation and focuses on the removal a target analyte from solution phase. Here we analyze photocatalytic action TiO2-nitrogen-doped graphene quantum dot (NGQD) composite analyte, phenol, using comprehensive multiphase NMR (CMP-NMR) which observes signals solid, solution, gel situ. Phenol preferentially interacts with photocatalyst compared to pure TiO2, increasing its effective concentration near catalyst surface degradation...
Alzheimer's disease (AD) is a complex, progressive neurodegenerative disorder, impacting millions of geriatric patients globally. Unfortunately, AD can only be diagnosed post-mortem, through the analysis autopsied brain tissue in human patients. This renders early detection and countering progression difficult. As progresses, metabolomic profile other organs change. These alterations detected peripheral systems (i.e., blood) such that biomarkers identified monitored with minimal invasion. In...
Due to the COVID-19 pandemic, social distancing restrictions are in place most public settings, and undergraduate laboratory is no exception. In order accommodate requirements, many exercises being redeployed an online format, which deprives students of experiential learning opportunities a real setting. To bridge this gap for exercises, open-source remote titration unit was created. This based on simple Raspberry Pi architecture equipped with webcam servo (a small motor allowing fine...
Abstract Synergism between different phases gives rise to chemical, biological or environmental reactivity, thus it is increasingly important study samples intact. Here, SASSY (SimultAneous Solid and Solution spectroscopY) introduced simultaneously observe (and differentiate) all in multiphase using standard, solid‐state NMR equipment. When monitoring processes, the traditional approach of studying solids liquids sequentially, can lead information non‐observed phase being missed. solves this...
In recent years there has been a renewed interest in benchtop NMR. Given their lower cost of ownership, smaller footprint, and ease use, they are especially suited as an educational tool. Here, new experiment targeted at upper-year undergraduates first-year graduate students follows the conversion D-glucose into ethanol low-field. First, high low-field data on compared learn both Hz ppm scales how J-coupling is field-independent. The then acquire own quantitative NMR datasets perform...
Nuclear Magnetic Resonance (NMR) spectroscopy is a non-invasive analytical technique which allows for the study of intact samples. Comprehensive Multiphase NMR (CMP-NMR) combines techniques and hardware from solution state solid to allow holistic analysis all phases (i.e. solutions, gels solids) in unaltered This first apply CMP-NMR deceased, organisms uses 13C enriched Daphnia magna (water fleas) as an example. D. are commonly used model environmental toxicology studies. As primary...
Abstract Benchtop NMR provides improved accessibility in terms of cost, space, and technical expertise. In turn, this encourages new users into the field spectroscopy. Unfortunately, many interesting samples education research, from beer to whole blood, contain significant amounts water that require suppression 1 H order recover sample information. However, due reduction chemical shift dispersion benchtop systems, signals are much closer resonance compared those a corresponding high‐field...
Comprehensive multiphase–nuclear magnetic resonance (CMP–NMR) is a non-invasive approach designed to observe all phases (solutions, gels, and solids) in intact samples using single NMR probe. Studies of dead living organisms are important understand processes ranging from biological growth environmental stress. Historically, such studies have utilized 1H-based phase editing for the detection soluble/swollen components 1H-detected 2D metabolite assignments/screening. However, require slow...
Abstract NMR applied to living organisms is arguably the ultimate tool for understanding environmental stress responses and can provide desperately needed information on toxic mechanisms, synergistic effects, sublethal impacts, recovery, biotransformation of xenobiotics. To perform in vivo spectroscopy, a flow cell system required deliver oxygen food while maintaining optimal line shape spectroscopy. In this tutorial, two such systems their constructions are discussed: (a) single pump...
The ability to visualize chemical processes can be an asset in a teaching laboratory. Despite the advances laboratory technology, apparatuses used of simple thermodynamics concepts, such as exothermic/endothermic reactions, historically remain unchanged and benefit from fresh perspective. recent incorporation thermal imaging cameras into chemistry has enabled students better grasp concepts thermodynamics. In fact, most visual learners will this approach, advocating its importance...
Abstract NMR/MRI are critical tools for studying molecular structure and interactions but suffer from relatively low sensitivity spectral overlap. Here, a Nuclear Magnetic Resonance (NMR) approach, termed DREAMTIME, is introduced that provides “a window” inside complex systems, capable of showing only what the user desires, with complete specificity. The chooses list molecules interest, approach detects those targets while all other invisible. demonstrated in whole human blood urine, small...
Elucidating the interactions between plastic nanoparticles and small molecules is important to understanding these as they occur in polluted waterways. For example, that breaks down into micro- nanoscale particles will interact with molecule pollutants are also present contaminated waters. Other components of natural water, such dissolved organic matter, influence interactions. Here we use a collection complementary NMR techniques examine binding polystyrene three common antibiotics,...
Comprehensive multiphase (CMP) NMR, first described in 2012, combines all of the hardware components necessary to analyze phases (solid, gel, and solution) samples their natural state. In combination with spectral editing experiments, it can fully differentiate study transfer chemical species across between phases, providing unprecedented molecular-level information unaltered systems. However, many samples, such as swollen soils, plants, small organisms, contain water, salts, ionic...
Comprehensive Multiphase NMR (CMP-NMR) is a recently developed technique capable of simultaneously observing different phases - solutions, gels, and solids while providing the chemical specificity traditional NMR. With this new tool, heterogeneous photocatalysis phenol by titanium dioxide (P25 TiO2) re-examined to gain information about occurrence reaction at regions between catalyst solution. It was found that proportion in changes over course photodegradation period. The photocatalyst...
Nuclear magnetic resonance (NMR) spectroscopy is commonly employed in a wide range of metabolomic research. Unfortunately, due to its relatively low sensitivity, smaller samples become challenging study by NMR. Cryoprobes can be used increase sensitivity cooling the coil and preamplifier, offering improvements ∼3 4x. Alternatively, microcoils mass improving sample filling proximity, along with decreased electrical resistance. combining two approaches not just technically challenging, but as...
Comprehensive multiphase NMR combines the ability to study and differentiate all phases (solids, gels, liquids) using a single probe. The general goal of CMP-NMR is intact environmental biological samples better understand conformation, organization, association, transfer between across phases/interfaces that may be lost with conventional sample preparation such as drying or solubilization. To date, studies have used 4 mm probes rotors. Here, larger 7 probehead introduced which provides ∼3...
Many key building blocks of life contain nitrogen moieties. Despite the prevalence nitrogen-containing metabolites in nature, 15N nuclei are seldom used NMR-based metabolite assignment due to their low natural abundance and lack comprehensive chemical shift databases. However, with advancements isotope labeling strategies, 13C enriched becoming more common metabolomic studies. Simple multidimensional nuclear magnetic resonance (NMR) experiments that correlate 1H via single bond 1JNH or...
The recent popularity of benchtop (BT) NMR systems has prompted its applications in undergraduate laboratories around the world. Owing to their low maintenance cost, due lack a superconducting magnetic core, and simple operation, these BT can fulfill many learning objectives outlined organic chemistry curricula. With variety currently available (e.g., 43, 60, 80, 100 MHz), it be overwhelming for instructors determine which system is appropriate needs. When used as structure elucidation tool,...
Plastic pollution is a growing environmental concern. In addition to posing hazards wildlife, micro- and nano-scale plastic particles can adsorb toxic small molecules from polluted waterways, which may be released when these are ingested by animals or humans. Previous NMR studies have examined the binding between surface-modified polystyrene nanoparticles amino acids as models for with variety of functional groups. These previous studies, however, could only examine liquid phase therefore...
HR-MAS NMR is a powerful tool, capable of monitoring molecular changes in intact heterogeneous samples. However, one the biggest limitations 1H its narrow spectral width which leads to considerable overlap complex natural DREAMTIME highly selective technique that allows users isolate suites metabolites from congested spectra. This permits targeted metabolomics by and ideal for specific processes. To date, has only been employed solution-state NMR, here it adapted applications. At high...