A5066841797- Glioma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Gene expression and cancer classification
- Cancer Research and Treatments
- Renal and related cancers
- Microfluidic and Capillary Electrophoresis Applications
- Transboundary Water Resource Management
- Immune cells in cancer
- International Maritime Law Issues
- MicroRNA in disease regulation
- Nanoplatforms for cancer theranostics
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Ion Transport and Channel Regulation
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
Johannes Gutenberg University Mainz
2023-2025
University of Luxembourg
2020-2023
University of Applied Sciences Mainz
2023
Laboratoire National de Santé
2018-2021
Biogem
2021
Centre Hospitalier de Luxembourg
2020
Patient-based cancer models are essential tools for studying tumor biology and the assessment of drug responses in a translational context. We report establishment large cohort unique organoids patient-derived orthotopic xenografts (PDOX) various glioma subtypes, including gliomas with mutations IDH1, paired longitudinal PDOX from primary recurrent tumors same patient. show that PDOXs enable long-term propagation patient represent clinically relevant avatars retain histopathological,...
Circulating cell-free DNA (cfDNA), circulating tumour (ctDNA) and inflammatory cytokines have prognostic predictive value in oncology. However, the effects of acute exercise on cfDNA levels are unknown. Here, we explore kinetics cfDNA, ctDNA upon an incremental test a pilot cohort cancer patients compared with healthy control subjects. Patients solid tumours (n = 12) age-matched subjects 6) were recruited to perform all-out cardiopulmonary bicycle test. Blood samples collected before (Pre),...
MicroRNAs (miRNAs), formed by cleavage of pre-microRNA the endoribonuclease Dicer, are critical modulators cell function post-transcriptionally regulating gene expression.Selective ablation Dicer in AQP2-expressing cells (DicerAQP2Cre+ mice) was used to investigate role miRNAs kidney collecting duct mice.The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due greatly reduced AQP2 AQP4 levels. Although epithelial sodium channel levels were decreased cortex increased...
Abstract Background Mutations in isocitrate dehydrogenase 1 or 2 (IDH1/2) define glioma subtypes and are considered primary events gliomagenesis, impacting tumor epigenetics metabolism. IDH enzyme activity is crucial for the generation of reducing potential normal cells, yet impact mutation on cellular antioxidant system not understood. The aim this study was to determine how glutathione (GSH), main brain, maintained IDH1-mutant gliomas, despite an altered NADPH/NADP balance. Methods...
Summary Digital polymerase chain reaction (dPCR) is an emerging technology that enables accurate and sensitive quantification of nucleic acids. Most available dPCR systems have two channel optics, with ad hoc software limited to the analysis single duplex assays. Although multiplexing strategies were developed, variable assay designs, systems, low DNA input data restricted ability for a universal automated clustering approach. To overcome these issues, we developed Cluster Predictor (dPCP),...
ABSTRACT Patient-derived cancer models are essential tools for studying tumor biology and preclinical interventions. Here, we show that glioma patient-derived orthotopic xenografts (PDOXs) enable long-term propagation of patient tumors represent clinically relevant avatars. We created a large collection PDOXs from primary recurrent gliomas with without mutations in IDH1 , which retained histopathological, genetic, epigenetic transcriptomic features no mouse-specific clonal evolution....