A5018878265- Renal and related cancers
- Ion Transport and Channel Regulation
- Dialysis and Renal Disease Management
- MicroRNA in disease regulation
- Genetic and Kidney Cyst Diseases
- Glycogen Storage Diseases and Myoclonus
- Chronic Kidney Disease and Diabetes
- Electrolyte and hormonal disorders
- Dementia and Cognitive Impairment Research
- Birth, Development, and Health
- Pancreatic function and diabetes
- Renal Diseases and Glomerulopathies
- COVID-19 and healthcare impacts
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Fish Ecology and Management Studies
- Cardiac, Anesthesia and Surgical Outcomes
- Blood Pressure and Hypertension Studies
- Gut microbiota and health
- Telemedicine and Telehealth Implementation
- Neuroendocrine regulation and behavior
- Neurological Complications and Syndromes
- COVID-19 diagnosis using AI
- Advanced Fluorescence Microscopy Techniques
- Renal cell carcinoma treatment
University of Campania "Luigi Vanvitelli"
2013-2025
Biogem
2016-2025
Regione Campania
2023
University of Naples Federico II
2013-2022
Federico II University Hospital
2020-2022
Ospedale Policlinico San Martino
2022
Aarhus University
2022
Universitätsmedizin Greifswald
2020-2021
Abstract Nephrogenic diabetes insipidus (NDI) is a rare tubulopathy characterized by urinary concentration defect due to renal resistance vasopressin. Loss-of-function mutations of vasopressin V2 receptor (V2R) gene ( AVPR2 ) the most common cause disease. We have identified five novel L86P, R113Q, C192S, M272R, and W323_I324insR from NDI-affected patients. Functional characterization these mutants revealed that R113Q C192S were normally localized at basolateral membrane polarized...
Glycogen storage disease XI, also known as Fanconi-Bickel syndrome (FBS), is a rare autosomal recessive disorder caused by mutations in the SLC2A2 gene that encodes glucose-facilitated transporter type 2 (GLUT2). Patients develop life-threatening renal proximal tubule dysfunction for which no treatment available apart from electrolyte replacement. To investigate pathogenesis of FBS, expression was ablated mouse kidney and HK-2 cells. GLUT2Pax8Cre+ mice developed time-dependent glycogen...
Mild cognitive impairment (MCI) is common in people with chronic kidney disease (CKD), and its prevalence increases progressive loss of function. MCI characterized by a decline performance greater than expected for an individual age education level but minimal instrumental activities daily living. Deterioration can affect one or several domains (attention, memory, executive functions, language, perceptual motor social cognition). Given the increasing disease, more CKD will also develop...
The distal nephron is a heterogeneous part of the composed by six different cell types, forming epithelium convoluted (DCT), connecting, and collecting duct. To dissect function these cells, knockout models specific for their unique marker have been created. However, since this develops at border between ureteric bud metanephric mesenchyme, specificity single markers has recently questioned. Here, mapping fate aquaporin 2 (AQP2) Na+-Cl- cotransporter (NCC)-positive cells using transgenic...
Department of Medical Translational Sciences, University Campania "Luigi Vanvitelli," Naples, Italy; and Biology Molecular Genetics Institute, Biogem, Ariano Irpino, Italy Correspondence: Dr. Francesco Trepiccione, email: [email protected] See related article, "A Snake Toxin Derivative for Treatment Hyponatremia Polycystic Kidney Diseases," on pages 181–192.
Abstract Potassium depletion affects AQP2 expression and the cellular composition of kidney collecting duct. This, in turn, contributes to development a secondary form nephrogenic diabetes insipidus hypokalemic nephropathy. Here we show that after 14 days potassium depletion, fraction A-type intercalated cells increases while principal decreases along outer medullary duct rats. The acquired novel distribution pattern forming rows attached each other. These morphological changes occur...
Cyclosporine A (CsA) is one of the most frequently used anticalcineurinic drugs for preventing graft rejection and autoimmune disease. Its use hampered by nephrotoxic effects, namely an impairment glomerular filtration rate (GFR) hypertension. Evidence suggests that reactive oxygen species (ROS) play a causal role in nephrotoxicity. The present study aims to investigate vivo effects new recombinant mitochondrial manganese-containing superoxide dismutase (rMnSOD), strong antioxidant, on...
Dicer is a crucial enzyme for the maturation of miRNAs. Mutations in gene are highly associated with Pleuro Pulmonary Blastoma-Family Dysplasia Syndrome (PPB-FDS, OMIM 601200), recently proposed to be renamed syndrome. Aside from pulmonary phenotype (blastoma), renal nephroma and thyroid goiter frequently part To investigate phenotype, conditional knockout (cKO) mice Pax8 expressing cells were generated. cKO progressively develop glomerulocystic coupled urinary concentration impairment,...
<b><i>Background:</i></b> MicroRNAs (miRNAs) are a family of short noncoding RNAs that play important roles in posttranscriptional gene regulation. miRNAs inhibit target expression by blocking protein translation or inducing mRNA degradation and therefore have the potential to modulate physiological pathological processes. <b><i>Summary:</i></b> In kidney, role organogenesis pathogenesis several diseases, including renal carcinoma, diabetic...
Focal and segmental glomerulosclerosis (FSGS) is a histological pattern frequently found in patients with nephrotic syndrome that often progress to end-stage kidney disease. The initial step development of this histologically defined entity injury ultimately depletion podocytes, highly arborized interdigitating cells on the glomerular capillaries important function for filtration barrier. Since there are still no causal therapeutic options, animal models needed develop new treatment...
MicroRNAs (miRNAs), formed by cleavage of pre-microRNA the endoribonuclease Dicer, are critical modulators cell function post-transcriptionally regulating gene expression.Selective ablation Dicer in AQP2-expressing cells (DicerAQP2Cre+ mice) was used to investigate role miRNAs kidney collecting duct mice.The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due greatly reduced AQP2 AQP4 levels. Although epithelial sodium channel levels were decreased cortex increased...
<b><i>Introduction:</i></b> Since the pandemic of COVID-19 started from December 2019, remarkable numbers infections and deaths associated with have been recorded worldwide. End-stage kidney disease patients on dialysis are particularly at high risk due to impairments in innate adaptive immune systems. Vaccination (DP) still remains challenging because variable response a low seroconversion rate compared healthy participants (HP). Therefore, it is urgently necessary...
Abstract Renal micropuncture, which requires the direct access to renal tubules, has for long time been technique of choice measure single nephron glomerular filtration rate (SNGFR) in animal models. This approach is challenging by virtue complex preparation and numerous technically difficult steps. The introduction intravital multiphoton microscopy (MPM) offers another SNGFR mean high laser-tissue penetration optical sectioning capacity. Previous MPM studies measuring vivo relied on fast...
Mutations in SLC37A4, which encodes the intracellular glucose transporter G6PT, cause rare glycogen storage disease type 1b (GSD1b). A long-term consequence of GSD1b is kidney failure, requires KRT. The main protein markers proximal tubule function, including NaPi2A, NHE3, SGLT2, GLUT2, and AQP1, are downregulated as part phenotype.We utilized an inducible mouse model GSD1b, TM-G6PT-/-, to show that accumulation plays a crucial role altering morphology function. To limit entry into cells...
Healthcare associated infections (HAI) are a major concern. Infections and sepsis prevalent causes of prolonged hospitalization, healthcare resources consumption, mortality. Infection prevention control (IPC) measures need to be encouraged with the purpose become standard protocol. Hand hygiene represents most important measure prevent spread infections. Poor hand is often due to: belief that use gloves replaces hygiene, lack good example from colleagues superiors, sinks difficult access or...
The I-κB kinase (IKK) subunit NEMO/IKKγ (NEMO) is an adapter molecule that critical for canonical activation of NF-κB, a pleiotropic transcription factor controlling immunity, differentiation, cell growth, tumorigenesis, and apoptosis. To explore the functional role NF-κB signaling in thyroid gland differentiation function, we have generated murine strain bearing genetic deletion NEMO locus thyroid. Here show thyrocyte-specific knock-out mice gradually develop hypothyroidism after birth,...
Endoderm-derived organs as liver and pancreas are potential targets for regenerative therapies, thus, there is great interest in understanding the pathways that regulate induction specification of this germ layer. Currently, knowledge molecular mechanisms guide vivo endoderm restricted by lack early specific markers. Nephrocan (Nepn) a gene whose expression characterizes stages murine (E7.5–11.5) encodes secreted N-glycosylated protein. In present study, we report identification new...
Immunosuppression induced by anticancer therapy in a COVID-19-positive asymptomatic patient with cancer may have devastating effect and, eventually, be lethal. To identify cases among patients receiving active treatment, the Federico II University Hospital Naples performs rapid serological tests addition to hospital standard clinical triage for COVID-19 infection.From 6 17 April 2020, all candidates chemotherapy, radiotherapy or target/immunotherapy, if negative at on day scheduled received...
Background Vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are highly effective. Nevertheless, immunocompromised participants were excluded from randomized controlled clinical trials. This study evaluates the efficacy and safety of Pfizer/BioNTech BNT162b2 (BNT162b2) vaccine in patients with breast gynecological cancer treated active anticancer therapy versus a control cohort healthy participants. Methods Immune responses to ( n = 44) or malignancy 6) on (28...
Background: Virtual reality (VR) experience is the most adopted form of video-gaming to reduce preoperative anxiety. This prospective randomized clinical trial aimed examine feasibility and efficacy VR in children undergoing elective surgery. Materials Methods: All patients older than 13 years scheduled for surgery between March June 2021 were enrolled. Preoperative consisted watching a 5-minute video using head-mounted display. Four parameters evaluated compared two groups: (1) patient...