A5049894748- Ion Transport and Channel Regulation
- Dialysis and Renal Disease Management
- Renal function and acid-base balance
- Electrolyte and hormonal disorders
- Ion channel regulation and function
- Diet and metabolism studies
- Chronic Kidney Disease and Diabetes
- Blood Pressure and Hypertension Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Dementia and Cognitive Impairment Research
- Renal and related cancers
- Neuroscience of respiration and sleep
- Neurological Disorders and Treatments
- Physiological and biochemical adaptations
- High Altitude and Hypoxia
- Neurological and metabolic disorders
- Nitric Oxide and Endothelin Effects
- Birth, Development, and Health
- Hemoglobin structure and function
- Connexins and lens biology
- Bone and Dental Protein Studies
- Erythropoietin and Anemia Treatment
- COVID-19 Clinical Research Studies
- Advanced Glycation End Products research
- Heart Rate Variability and Autonomic Control
Aarhus University
2015-2024
University of Zurich
2023
Target (United States)
2023
Institute for Biomedicine
2006
Recent studies have demonstrated that a novel anion exchanger, pendrin, is expressed in the apical domain of type B intercalated cells mammalian collecting duct. The purpose this study was 1) to determine expression and distribution pendrin along duct connecting tubule mouse rat kidney establish whether non-A-non-B 2) intracellular localization different populations by immunoelectron microscopy. A peptide-derived affinity-purified antibody generated specifically recognized immunoblots...
Vascular endothelial growth factor A (VEGFA) production by podocytes is critical for glomerular health. VEGFA also expressed in tubular epithelial cells kidney; however, its physiologic role the tubule has not been established. Using targeted transgenic mouse models, we found that Vegfa specific along nephron, whereas expression of receptor (Kdr/Vegfr2) largely restricted to adjacent peritubular capillaries. Embryonic deletion did affect systemic levels, renal abundance was markedly...
ABSTRACT Cholinergic synapses are widespread throughout the human central nervous system. Their high density in thalamus, neocortex, limbic system and striatum suggests that cholinergic transmission plays a vital role memory, attention, learning other higher cognitive functions. As result, brain's occupies position research on normal cognition age-related decline, including dementias such as Alzheimer's disease. In addition to its brain, neuronal pathways essential for physiological...
Arginine‐vasopressin (AVP) regulates water reabsorption in renal collecting duct principal cells. Its binding to G s ‐coupled vasopressin V2 receptors increases cyclic AMP (cAMP) and subsequently elicits the redistribution of channel aquaporin‐2 (AQP2) from intracellular vesicles into plasma membrane (AQP2 shuttle), thereby facilitating primary urine. The AQP2 shuttle is a paradigm for cAMP‐dependent exocytic processes. Using sections rat kidney, AQP2‐expressing cell line CD8, cells, we...
Mature enamel consists of densely packed and highly organized large hydroxyapatite crystals. The molecular machinery responsible for the formation fully matured is poorly described but appears to involve oscillative pH changes at surface. We conducted an immunohistochemical investigation selected transporters related proteins in multilayered rat incisor organ. Connexin 43 (Cx-43) found papillary cells ameloblasts, whereas Na(+)-K(+)-ATPase heavily expressed during maturation cell layer only....
A link between brain dysfunction and advanced kidney disease was first noted in the 1930s.Terms such as "uremic encephalopathy", "dialysis disequilibrium syndrome" dementia" were later used to describe impairment of function severe uremic states or during rapid initiation early dialysis regimens.Recent data, however, suggest damage can be present at earlier stages chronic (CKD), manifest with a continuum from mild involvement (mild cognitive impairment, MCI) up clinically relevant...
The anion exchanger pendrin is present in the apical plasma membrane of type B and non-A-non-B intercalated cells cortical collecting duct (CCD) connecting tubule involved HCO(3)(-) secretion. In this study, we investigated whether abundance subcellular localization are regulated response to experimental metabolic acidosis alkalosis with maintained water sodium intake. NH(4)Cl loading (0.033 mmol NH(4)Cl/g body wt for 7 days) dramatically reduced 22 +/- 4% control values (n = 6, P < 0.005)....
We have previously demonstrated that vasopressin increases the water permeability of inner medullary collecting duct (IMCD) by inducing trafficking aquaporin-2 to apical plasma membrane and this response is dependent on intracellular calcium mobilization calmodulin activation. Here, we address hypothesis mediated in part through activation calcium/calmodulin-dependent myosin light chain kinase (MLCK) regulation non-muscle II. Immunoblotting immunocytochemistry presence MLCK, regulatory...
Tissue kallikrein (TK) is a serine protease synthetized in renal tubular cells located upstream from the collecting duct where potassium balance regulated. Because secretion of TK promoted by K + intake, we hypothesized that this enzyme might regulate plasma concentration ([K ]). We showed wild-type mice and excretion increase parallel after single meal, representing an acute load, whereas aldosterone not modified. Using synthase-deficient mice, confirmed control aldosterone-independent....
There seems to be a lack of consensus on the necessity and modality psychological specifically cognitive assessment candidates for kidney transplantation. Both points are often delegated individual hospitals/centres, whereas international guidelines inconsistent. We think it is essential investigate professionals' opinions advance towards consistent clinical practice. This paper presents results an survey among professionals, mainly nephrologists from CONNECT (Cognitive decline in...
The loop diuretic furosemide inhibits NaCl reabsorption in the thick ascending limb (TAL). In addition, acidifies urine, which is traditionally explained by increased Na+ loading to distal tubule causing an activation of H+ secretion via H+-ATPase α-intercalated cells. inability acidify urine response serves diagnose renal tubular acidosis (dysfunction cells). Since TAL important for acid/base regulation, we speculated that it involved furosemide-induced urinary acidification. Luminal (100...
Glycogen storage disease XI, also known as Fanconi-Bickel syndrome (FBS), is a rare autosomal recessive disorder caused by mutations in the SLC2A2 gene that encodes glucose-facilitated transporter type 2 (GLUT2). Patients develop life-threatening renal proximal tubule dysfunction for which no treatment available apart from electrolyte replacement. To investigate pathogenesis of FBS, expression was ablated mouse kidney and HK-2 cells. GLUT2Pax8Cre+ mice developed time-dependent glycogen...
Mild cognitive impairment (MCI) is common in people with chronic kidney disease (CKD), and its prevalence increases progressive loss of function. MCI characterized by a decline performance greater than expected for an individual age education level but minimal instrumental activities daily living. Deterioration can affect one or several domains (attention, memory, executive functions, language, perceptual motor social cognition). Given the increasing disease, more CKD will also develop...
The electroneutral Na(+)-dependent HCO3- transporter NBCn1 is strongly expressed in the basolateral membrane of rat medullary thick ascending limb cells (mTAL) and up-regulated during NH4(+)-induced metabolic acidosis. Here we used vitro perfusion BCECF video-imaging mTAL tubules to investigate functional localization regulation influx Tubule acidification was induced by removing luminal Na+ (DeltapHi: 0.88 +/- 0.11 pH units, n = 10). Subsequently solution changed CO2/HCO3- buffer with...
Extracellular nucleotides are important regulators of epithelial ion transport. Here we investigated nucleotide-mediated effects on colonic NaCl secretion and the signal transduction mechanisms involved. Basolateral UDP induced a sustained activation Cl(-) secretion, which was completely inhibited by 293B, specific inhibitor cAMP-stimulated basolateral KCNQ1/KCNE3 K(+) channels. We therefore speculated that P2Y(6) receptor could increase cAMP. Indeed elevated cAMP in isolated crypts....
Extracellular nucleotides are important regulators of epithelial ion transport. Here we investigated nucleotide-mediated effects on colonic NaCl secretion and the signal transduction mechanisms involved. Basolateral UDP induced a sustained activation Cl– secretion, which was completely inhibited by 293B, specific inhibitor cAMP-stimulated basolateral KCNQ1/KCNE3 K+ channels. We therefore speculated that P2Y6 receptor could increase cAMP. Indeed elevated cAMP in isolated crypts. identified an...
The renin-angiotensin system is well known to be involved in the pathophysiological changes renal function after obstruction of ureter. Previously, we demonstrated that bilateral ureteral (BUO) associated with dramatic expression both sodium transporters and aquaporin water channels (AQPs). We now examined effects AT(1)-receptor antagonist candesartan on dysregulation AQPs key rats subjected 24-h BUO followed 2 days release (BUO-2R). Consistent previous observations, BUO-2R resulted a...