A5060002497- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Protein purification and stability
- Microtubule and mitosis dynamics
- Bacterial Genetics and Biotechnology
- Galectins and Cancer Biology
- Genetics, Aging, and Longevity in Model Organisms
- Mosquito-borne diseases and control
- Trace Elements in Health
- Crystallization and Solubility Studies
- RNA Research and Splicing
- Nuclear Structure and Function
- Toxin Mechanisms and Immunotoxins
- Advanced Biosensing Techniques and Applications
- Fungal and yeast genetics research
- Cell Adhesion Molecules Research
- interferon and immune responses
- Protein Degradation and Inhibitors
- HIV Research and Treatment
- Insect and Pesticide Research
Inserm
2010-2024
Institut de Recherche en Cancérologie de Montpellier
2014-2024
Institut Regional du Cancer de Montpellier
2014-2015
Université de Montpellier
2014-2015
Vicomtech
2014
eHealth Africa
2014
The University of Queensland
2006-2013
Centre National de la Recherche Scientifique
2005-2011
Centre de Biologie Structurale
2010-2011
Australian Research Council
2007
Abstract Human Eg5, a member of the kinesin superfamily, plays key role in mitosis, as it is required for formation bipolar spindle. We describe here first vitro microtubule-activated ATPase-based assay identification small-molecule inhibitors Eg5. screened preselected libraries obtained from National Cancer Institute and identified S-trityl-l-cysteine most effective Eg5 inhibitor with an IC50 1.0 μmol/L inhibition basal ATPase activity 140 nmol/L activity. Subsequent cell-based assays...
Triple-negative breast cancer (TNBC) treatment is currently restricted to chemotherapy. Hence, tumor-specific molecular targets and/or alternative therapeutic strategies for TNBC are urgently needed. Immunotherapy emerging as an exciting option patients. The aspartic protease cathepsin D (cath-D), a marker of poor prognosis in (BC), overproduced and hypersecreted by human BC cells. This study explores whether cath-D tumor cell-associated extracellular biomarker potent target antibody-based...
Antibody molecules are able to recognize any antigen with high affinity and specificity. To get insight into the molecular diversity at source of this functional diversity, we compiled analyzed a non-redundant aligned collection 227 structures antibody-antigen complexes. Free energy binding all residue side chains was quantified by computational alanine scanning, allowing first large-scale quantitative description antibody paratopes. This demonstrated that as few 8 residues among 30 key...
Siphophage SPP1 infects the gram-positive bacterium Bacillus subtilis using its long non-contractile tail and tail-tip. Electron microscopy (EM) previously allowed a low resolution assignment of most orf products belonging to these regions. We report here structure distal protein (Dit, gp19.1). The combination x-ray crystallography, EM, light scattering established that Dit is back-to-back dimer hexamers. However, fitting in virion EM maps was only possible with hexamer located between...
Crystallization is a major bottleneck in the process of macromolecular structure determination by X-ray crystallography. Successful crystallization requires formation nuclei and their subsequent growth to crystals suitable size. Crystal generally occurs spontaneously supersaturated solution as result homogenous nucleation. However, typical sparse matrix screening experiment, precipitant protein concentration are not sampled extensively, supersaturation conditions for nucleation often...
The SPP1 siphophage uses its long non-contractile tail and tip to recognize infect the Gram-positive bacterium Bacillus subtilis. tail-end cap attached are critical components for host recognition opening of tube genome exit. In present work, we determined cryo-electron microscopic (cryo-EM) structure a complex formed by protein gp19.1 (Dit) N terminus downstream in genome, gp21(1-552) (Tal). This assembles two back-to-back stacked ring hexamers, interacting loosely, trimers with at both...
Systemic sclerosis (SSc) is an autoimmune, inflammatory and fibrotic disease with limited treatment options. Developing new therapies therefore crucial to address patient needs. To this end, we focused on galectin-3 (Gal-3), a lectin known be associated several pathological processes seen in SSc. Using RNA sequencing of whole-blood samples cross-sectional cohort 249 patients SSc, Gal-3 its interactants defined strong transcriptomic fingerprint severity, pulmonary cardiac malfunctions,...
Abstract Context: The involvement of calcium signaling in tumor progression has been well documented the literature. In particular, channel TRPV6 identified as a key player physiopathology solid tumors. At transcriptomic level, is overexpressed many cancers such prostate, pancreatic and ovarian underscoring its potential new therapeutic target. While some TRPV6-targeting antagonistic peptides have reached clinical stage, no antibody candidate currently development. Leveraging phage display...
Ran-GTP interacts strongly with importin-beta, and this interaction promotes the release of importin-alpha-nuclear localization signal cargo from importin-beta. Ran-GDP also but is 4 orders magnitude weaker than Ran-GTP.importin-beta interaction. Here we use yeast complement nuclear import proteins to show that between importin-beta dissociation GDP Ran. The Ran-GDP-importin-beta complex stabilizes complex, which cannot be dissociated by importin-alpha. Although Ran has a higher affinity for...
Circular dichroism (CD) spectroscopy beamlines at synchrotrons produce dramatically higher light flux than conventional CD instruments. This property of synchrotron radiation circular (SRCD) results in improved signal-to-noise ratios and allows data collection to lower wavelengths, characteristics that have led the development novel SRCD applications. Here we describe use study protein complex formation, specifically evaluating formed between carboxypeptidase A its inhibitor latexin. Crystal...
Intrabodies, when expressed in cells after genetic fusion to fluorescent proteins, are powerful tools study endogenous protein dynamics inside cells. However, it remains challenging determine the conditions for specific imaging and precise labelling of target antigen with such intracellularly antibody fragments. Here, we show that single‐chain Fv (scFv) fragments can be generated specifically recognize proliferating cell nuclear (PCNA) living cancer After selection by phage display,...
The caspase recruitment domain (CARD) is present in death-domain superfamily proteins involved inflammation and apoptosis. BinCARD named for its ability to interact with Bcl10 inhibit downstream signalling. Human expressed as two isoforms that encode the same N-terminal CARD region but which differ considerably their C-termini. Both are immune cells, although BinCARD-2 much more highly expressed. Crystals of fold common both had low symmetry (space group P1). Molecular replacement was...
TRPV6 calcium channel is a prospective target in prostate cancer (PCa) since it not expressed healthy while its expression increases during progression. Despite the role of PCa cell survival and apoptotic resistance has been already established, no reliable tool to vivo thus reduce tumor burden known date. Here we report generation mouse monoclonal antibody mAb82 raised against extracellular epitope pore region channel. inhibited currents by 90% at 24 µg/ml dose-dependent manner decreasing...
Background Protein crystallisation screening involves the parallel testing of large numbers candidate conditions with aim identifying suitable as a starting point for production diffraction quality crystals. Generally, condition is performed in 96-well plates. While previous studies have examined effects protein construct, purity, or ingredients on crystallisation, few effect plate. Methodology/Principal Findings We statistically rigorous examination and evaluated interactions between...
Kinesins form a superfamily of molecular motors involved in cell division and intracellular transport. Twenty kinesins have been found the Caenorhabditis elegans genome, four these belong to kinesin-14 subfamily, i.e., with C-terminal motor domains. Three kinesin-14s, KLP-15, KLP-16, KLP-17, distinct subgroup which KLP-15 KLP-16 are more than 90% identical appear be related by relatively recent gene duplication. They essential for meiotic spindle organization chromosome segregation, mostly...