A5062921962- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Immune Response and Inflammation
- bioluminescence and chemiluminescence research
- Tracheal and airway disorders
- Bacillus and Francisella bacterial research
- Bacterial Identification and Susceptibility Testing
- Dysphagia Assessment and Management
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- NF-κB Signaling Pathways
- RNA and protein synthesis mechanisms
- Tumors and Oncological Cases
- Cardiac tumors and thrombi
- Advanced biosensing and bioanalysis techniques
- Anorectal Disease Treatments and Outcomes
- Yersinia bacterium, plague, ectoparasites research
- Glioma Diagnosis and Treatment
- Diphtheria, Corynebacterium, and Tetanus
- Chromosomal and Genetic Variations
Lenox Hill Hospital
2018-2024
Northwell Health
2018-2024
Hofstra University
2022
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2022
Boston University
2014-2019
Czech Academy of Sciences, Institute of Microbiology
2013-2017
Pulmonary Associates
2015-2017
Dentsply Sirona (United States)
2017
New York University
2008-2015
Office of Infectious Diseases
2010-2014
Staphylococcus aureus is capable of infecting nearly every organ in the human body. In order to infiltrate and thrive such diverse host tissues, staphylococci must possess remarkable flexibility both metabolic virulence programs. To investigate genetic requirements for bacterial survival during invasive infection, we performed a transposon sequencing (TnSeq) analysis S. experimental osteomyelitis. TnSeq identified 65 genes essential staphylococcal infected bone an additional 148 mutants with...
Campylobacter jejuni, a gram-negative, invasive organism, is common cause of food-borne bacterial diarrheal disease. However, the relationship between C. jejuni and innate immune system not well described. To better characterize host defense against we investigated ability nitric oxide/reactive nitrogen species to kill two strains jejuni. viability was measured after exposure reactive produced biochemically as acidified nitrite by bone marrow-derived macrophages. We report that caused...
Staphylococcus aureus organisms vary in the function of staphylococcal virulence regulator gene agr. To test for a relationship between agr and transmission S. aureus, we determined prevalence genetic basis dysfunction among nosocomial methicillin-resistant (MRSA) an area MRSA endemicity. Identical inactivating mutations were not detected epidemiologically unlinked clones within or hospitals. Additionally, most mutants had single mutations, indicating that they short lived. Collectively,...
Inactivating mutations in the Staphylococcus aureus virulence regulator agr are associated with worse outcomes bacteremic patients. However, whether dysfunction is primarily a cause or consequence of early bacteremia unknown. Analysis 158 paired S. clones from blood and nasal carriage sites individual patients revealed that recovery an agr-defective mutant was usually predicted by functionality isolates. Many agr-positive isolates produced low levels hemolytic toxins, but were similar to...
Summary Staphylococcus aureus overproduces a subset of immunomodulatory proteins known as the staphylococcal superantigen‐like (Ssls) under conditions pore‐mediated membrane stress. In this study we demonstrate that overproduction Ssls during stress is due to impaired activation two‐component module quorum‐sensing accessory gene regulator (Agr) system. Agr‐dependent repression ssl expression indirect and mediated by transcription factor repressor toxins (Rot). Surprisingly, observed Rot...
The current study identified bacterial factors that may improve management of methicillin-resistant Staphylococcus aureus (MRSA) nosocomial pneumonia. Isolates were obtained from 386 patients enrolled in a randomized, controlled antibiotic efficacy. screened for production virulence and vancomycin susceptibility. After adjustment host such as severity illness treatment modality, cytotoxic activity was strongly inversely associated with mortality; however, it had no effect on clinical cure....
Cigarette smoking increases risk for multiple diseases. MicroRNAs regulate gene expression and may play a role in smoking-induced target organ damage. We sought to describe microRNA signature of cigarette relate it smoking-associated clinical phenotypes, expression, lung inflammatory signaling.
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H2O2, a crucial host defense against S. aureus. We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion resulted decreased ATP levels and growth, despite increased rates...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Acute bacterial pneumonia is a significant public health concern worldwide. Understanding the signals coordinating lung innate immunity may foster development of therapeutics that limit tissue damage and promote host defense. We have previously shown messenger RNA expression IL-6 family cytokine oncostatin-M (OSM) significantly elevated in response to stimuli. However, its physiological significance during unknown. Here we demonstrate OSM rapidly increased airspaces mice after pulmonary...
In bacterial pneumonia, lung damage resulting from epithelial cell injury is a major contributor to the severity of disease and, in some cases, can lead long-term sequelae, especially setting severe or acute respiratory distress syndrome. Leukemia inhibitory factor (LIF), member IL-6 cytokine family, critical determinant tissue protection during but cellular sources LIF and signaling pathways leading its production infected are not known. Here, we demonstrate that epithelium, specifically...
Pneumonia and infection-induced sepsis are worldwide public health concerns. Both pathologies elicit systemic inflammation induce a robust acute-phase response (APR). Although APR activation is well regarded as hallmark of infection, the direct contributions liver to pulmonary defense during remain unclear. By targeting STAT3-dependent changes in liver, we evaluated role STAT3 activity promoting host context pneumonia. We employed two-hit endotoxemia/pneumonia model, whereby administration...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H 2 O , a crucial host defense against S. . We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion resulted decreased ATP levels and growth, despite increased rates respiration or...
ABSTRACT Staphylococcus aureus infections are a significant cause of morbidity and mortality in health care settings. S. clinical isolates vary the function accessory gene regulator ( agr ), which governs expression virulence determinants, including surface exoproteins, while activity has been correlated with patient outcome treatment efficiency. Here we describe duplex real-time nucleic acid sequence-based amplification (NASBA) detection quantification platform for rapid determination...
Alveolar macrophages orchestrate pulmonary innate immunity and are essential for early immune surveillance clearance of microorganisms in the airways. Inflammatory signaling must be sufficiently robust to promote host defense but limited enough prevent excessive tissue injury. Macrophages lungs utilize multiple transcriptional post-transcriptional mechanisms inflammatory gene expression delicately balance elaboration mediators. RNA terminal uridyltransferases (TUTs), including closely...
P-element-induced wimpy testes (Piwi) proteins are known for suppressing retrotransposon activation in the mammalian germline. However, whether Piwi protein or Piwi-dependent functions occur soma is unclear. Contrary to germline-restricted expression, we observed that Piwi-like Miwi2 mRNA indeed expressed epithelial cells of lung adult mice and it induced during pneumonia. Further investigation revealed MIWI2 localized cytoplasm a discrete population multiciliated airway cells. Isolation...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H 2 O , a crucial host defense against S. . We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion increased both respiration and fermentation but decreased ATP levels growth,...
Zinc finger protein 36, C3H type-like 1 (ZFP36L1) is one of several Finger Protein 36 (Zfp36) family members, which bind AU rich elements within 3′ untranslated regions (UTRs) to negatively regulate the post-transcriptional expression targeted mRNAs. The prototypical member family, Tristetraprolin (TTP or ZFP36), has been well-studied in context inflammation and plays an important role repressing pro-inflammatory transcripts such as TNF-α. Much less known about other none have studied...
Aortopulmonary paragangliomas are rare, neural crest-derived tumours that arise in the aortopulmonary window and present a significant surgical challenge due to their proximity major vessels. In relatively few cases reported study, thoracotomy video-assisted thorascopic surgery (VATS) approaches have been utilized for treatment of such masses. this report, we describe application totally endoscopic robotic-assisted VATS approach excision an paraganglioma.