A5051879659- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- bioluminescence and chemiluminescence research
- Bacillus and Francisella bacterial research
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Microbial Metabolic Engineering and Bioproduction
- Infective Endocarditis Diagnosis and Management
- Bacterial Genetics and Biotechnology
- Eosinophilic Esophagitis
- Streptococcal Infections and Treatments
- Oral microbiology and periodontitis research
- Computational Drug Discovery Methods
- Antibiotic Resistance in Bacteria
- Polyomavirus and related diseases
- Parasites and Host Interactions
- Yersinia bacterium, plague, ectoparasites research
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Salivary Gland Disorders and Functions
- Infections and bacterial resistance
- Listeria monocytogenes in Food Safety
University of Nebraska Medical Center
2018-2025
New York University
2023-2025
NYU Langone Health
2023-2025
Central South University
2024
West China Medical Center of Sichuan University
1999
Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH3 CO2 from urea, is key to pH homeostasis bacterial pathogens under acidic stress nitrogen limitation. However, the function of urease S. niche colonization metabolism has not been extensively studied. We discovered essential for viability urea-rich environments weak acid stress. The regulation transcription by CcpA, Agr, CodY was identified this study, implying a...
ABSTRACT Depletion of microbiota increases susceptibility to gastrointestinal colonization and subsequent infection by opportunistic pathogens such as methicillin-resistant Staphylococcus aureus (MRSA). How the absence gut impacts evolution MRSA is unknown. The present report used germ-free mice investigate evolutionary dynamics in microbiota. Through genomic analyses competition assays, we found that adapts microbiota-free through sequential genetic mutations structural changes enhance...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H2O2, a crucial host defense against S. aureus. We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion resulted decreased ATP levels and growth, despite increased rates...
Staphylococcus epidermidis is a commensal bacterium that resides on our skin. As commensal, it protects humans from bacterial pathogens through variety of mechanisms. However, also significant cause biofilm infections due to its ability bind plastic. Polysaccharide intercellular adhesin component biofilm, and we propose the expression this polysaccharide beneficial in certain host niches, such as providing extra strength when colonizing lumen catheter, detrimental others, colonization skin...
ABSTRACT Severe COVID-19 has been associated with coinfections bacterial and fungal pathogens. Notably, patients who develop Staphylococcus aureus bacteremia exhibit higher rates of mortality than those infected either pathogen alone. To understand this clinical scenario, we collected examined S. blood respiratory isolates from a hospital in New York City during the early phase pandemic both SARS-CoV-2+ SARS-CoV-2− patients. Whole genome sequencing these revealed broad phylogenetic diversity...
Abstract Whole genome sequencing (WGS) and clinical review were used to characterize 14 cases of central line-associated bloodstream infection (CLABSI) due Staphylococcus epidermidis . WGS, which demonstrated disparate strains, suggested that 42.9% S. CLABSI contamination, while 57.1% contamination events.
Gastrointestinal (GI) colonization by methicillin-resistant Staphylococcus aureus (MRSA) is associated with a high risk of transmission and invasive disease in vulnerable populations. The immune microbial factors that permit GI remain unknown. Male sex correlated enhanced nasal carriage, skin soft tissue infections, bacterial sepsis. Here, we established mouse model sexual dimorphism during MRSA. Our results show contrast to male mice were susceptible persistent colonization, female rapidly...
Gastrointestinal (GI) colonization by methicillin-resistant Staphylococcus aureus (MRSA) is associated with a high risk of transmission and invasive disease in vulnerable populations. The immune microbial factors that permit GI remain unknown. Male sex correlated enhanced nasal carriage, skin soft tissue infections, bacterial sepsis. Here, we established mouse model sexual dimorphism during MRSA. Our results show contrast to male mice were susceptible persistent colonization, female rapidly...
Isotopically labeling a metabolite and tracing its metabolic fate has provided invaluable insights about the role of metabolism in human diseases addition to variety other issues. 13C-labeled tracers or unlabeled 1H-based NMR experiments are currently most common application metabolomics studies. Unfortunately, coverage metabolome been consequently limited abundant carbon-containing metabolites. To expand enhance impact studies, we present protocol for 15N-labeled tracer that may also be...
Abstract Staphylococcus aureus is a metabolically versatile pathogen that colonizes nearly all organs of the human body. A detailed and comprehensive knowledge staphylococcal metabolism essential to understand its pathogenesis. To this end, we have reconstructed experimentally validated an updated enhanced genome-scale metabolic model S. USA300_FPR3757. The combined genome annotation data, reaction stoichiometry, regulation information from biochemical databases previous strain-specific...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H 2 O , a crucial host defense against S. . We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion resulted decreased ATP levels and growth, despite increased rates respiration or...
The bacterial microbiota promotes the life cycle of intestine-dwelling whipworm Trichuris by mediating hatching parasite eggs ingested mammalian host. Despite enormous disease burden associated with colonization, mechanisms underlying this transkingdom interaction have been obscure. Here, we used a multiscale microscopy approach to define structural events bacteria-mediated for murine model muris . Through combination scanning electron (SEM) and serial block face SEM (SBFSEM), visualized...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H 2 O , a crucial host defense against S. . We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion increased both respiration and fermentation but decreased ATP levels growth,...
Eubacterium species are a group of obligated anaerobic gram-positive bacilli that recognized as commensals the gastrointestinal tract flora. Cases bacteremia mediated by rare. This report describes case caused callanderi in an 82-year-old female with history cecal perforation secondary to obstructing sigmoid stricture. The results showed utility using whole genome sequencing identify causative agent and underlined significance organisms diagnostic microbiology practice perform antimicrobial...
Gastrointestinal (GI) colonization by methicillin-resistant Staphylococcus aureus (MRSA) is associated with a high risk of transmission and invasive disease in vulnerable populations. The immune microbial factors that permit GI remain unknown. Male sex correlated enhanced nasal carriage, skin soft tissue infections, bacterial sepsis. Here, we established mouse model sexual dimorphism during MRSA. Our results show contrast to male mice were susceptible persistent colonization, female rapidly...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H2O2, a crucial host defense against S. aureus. We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion increased both respiration and fermentation but decreased ATP levels growth,...
The agr quorum-sensing system links Staphylococcus aureus metabolism to virulence, in part by increasing bacterial survival during exposure lethal concentrations of H 2 O , a crucial host defense against S. . We now report that protection surprisingly extends beyond post-exponential growth the exit from stationary phase when is no longer turned on. Thus, can be considered constitutive protective factor. Deletion increased both respiration and aerobic fermentation but decreased ATP levels...