A5012766730- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Advanced Proteomics Techniques and Applications
- Geomechanics and Mining Engineering
- Nanoplatforms for cancer theranostics
- Endoplasmic Reticulum Stress and Disease
- Supramolecular Self-Assembly in Materials
- Geoscience and Mining Technology
- Advanced Sensor and Control Systems
- Photonic and Optical Devices
- Prion Diseases and Protein Misfolding
- Advanced Biosensing Techniques and Applications
- Hip disorders and treatments
- Neuroscience and Neuropharmacology Research
- Simulation and Modeling Applications
- Click Chemistry and Applications
- Wireless Sensor Networks and IoT
- Ubiquitin and proteasome pathways
- Higher Education and Teaching Methods
- Biotin and Related Studies
- Civil and Geotechnical Engineering Research
- Industrial Technology and Control Systems
- Photoreceptor and optogenetics research
- Semiconductor Lasers and Optical Devices
- Bone and Joint Diseases
La Trobe University
2018-2025
Institute for Molecular Science
2018-2021
Harbin University
2021
Peking University
2017-2019
Beijing Agricultural Machinery Research Institute
2005
The exosome functions in the degradation of diverse RNA species, yet how it is negatively regulated remains largely unknown. Here, we show that NRDE2 forms a 1:1 complex with MTR4, nuclear cofactor critical for recruitment, via conserved MTR4-interacting domain (MID). Unexpectedly, mainly localizes speckles, where inhibits MTR4 recruitment and degradation, thereby ensures efficient mRNA export. Structural biochemical data revealed interacts MTR4's key residues, locks closed conformation,...
Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation unfolded proteins, which has been found associate with a number disease conditions including neurodegenerative diseases, diabetes inflammation. Here we report maleimide-functionalized tetraphenylethene (TPE)-derivatized fluorescent dye, TPE-NMI, shows fluorescence turn-on property upon reacting proteins in vitro live cells under proteostatic stress conditions. The level be measured by flow cytometry...
Impairment of the protein quality control network leads to accumulation unfolded and aggregated proteins. Direct detection in cells may provide possibility for early diagnosis neurodegenerative diseases. Here a new platform based on peptide-conjugated thiol-reactive aggregation-induced emission fluorogen (AIEgen), named MI-BTD-P (or D1), labeling tracking proteins is reported. In vitro experiments with model show that non-fluorescent D1 only becomes highly fluorescent when reacted thiol...
Trypsin is one of the most important enzymes digestive system produced by pancreatic acinar cells. Abnormal trypsin activity will affect function, resulting in corresponding pathological changes human body. Herein, we present a strategy based on ensemble novel dual warhead probe HPC-ESF and natural substrate bovine serum albumin (BSA) for detection including real urine samples. The β-arylethenesulfonyl bearing nonemissive when dissolved aqueous solution but becomes highly fluorescent upon...
A new method using a single cell-permeable biochromic fluorescent dye to differentiate, quantify and image both live dead cells is reported.
Abstract Background There is now ample evidence suggesting that extracorporeal shock wave therapy (ESWT) can improve hip mobility and reduce pain in patients with osteonecrosis of the femoral head (ONFH). The ability ESWT to cure bone marrow edema syndrome (BMES) ONFH, 12 weeks after initial course ESWT, needs be verified further more relevant clinical research-based should consolidated. This study aimed evaluate efficacy for BMES caused by ONFH. Methods retrospective cohort included 67 ONFH...
NIAD-4 derivatives with modular groups are reported, enabling spectral tuning while retaining binding to fibrillar protein structures. Using NIAD-CNOET, cells treated preformed α-synuclein fibrils could be distinguished via flow cytometry.
Collapse of proteostasis has been found in a number disease conditions including neurodegeneration, diabetes and inflammation. To assess protein unfoldedness as measure capacity, maleimide-functionalized tetraphenylethene, TPE-NMI, was synthesized which shows fluorescence turn-on property upon reacting with unfolded proteins vitro live cells under proteostatic stress conditions. The level can be measured by using flow cytometry visualized confocal microscopy. More information the Full Paper...