A5075939927- Renin-Angiotensin System Studies
- Renal and Vascular Pathologies
- Diabetes Treatment and Management
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Nitric Oxide and Endothelin Effects
- Chronic Kidney Disease and Diabetes
- Hormonal Regulation and Hypertension
- Spaceflight effects on biology
- Pharmacological Receptor Mechanisms and Effects
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- Photodynamic Therapy Research Studies
- Rheumatoid Arthritis Research and Therapies
- Light effects on plants
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Receptor Mechanisms and Signaling
- DNA and Nucleic Acid Chemistry
- Acute Kidney Injury Research
- Renal Transplantation Outcomes and Treatments
- Stress Responses and Cortisol
- Antifungal resistance and susceptibility
- Planetary Science and Exploration
- Systemic Lupus Erythematosus Research
Semmelweis University
2015-2024
Hungarian Academy of Sciences
2010-2024
The University of Texas at Austin
2024
Széchenyi István University
2024
Tufts Medical Center
2023
Methodist Hospital
2021
Eötvös Loránd University
2021
Montavid Thermodynamic Research Group
2015-2020
TCM-Klinik Bad Kötzting
2019
University Medical Center Brackenridge
2018
Carotid-femoral pulse wave velocity is an established method for characterizing aortic stiffness, individual predictor of cardiovascular mortality in adults. Normal values the pediatric population derived from a large data collection have yet to be available. The aim this study was create reference database and characterize factors determining children teenagers. measured by applanation tonometry. Reference tables velocities obtained 1008 healthy subjects (aged between 6 20 years; 495 males)...
A highly sensitive and specific diagnostic test for Brucella based on polymerase chain reaction is under development in our laboratory. commercially available PCR kit was used to create primers that allowed the amplification of a 635 bp fragment 43 kDa outer membrane protein gene from abortus strain 19. We successfully amplified cloned present pMS64 plasmid genomic S19 DNA. The DNA easily detected by agarose gel electrophoresis. Using both Br. purified as template each component adjusted...
Increased activation of the renin-angiotensin-aldosterone system (RAAS) and elevated growth factor production are crucial importance in development renal fibrosis leading to diabetic kidney disease. The aim this study was provide evidence for antifibrotic potential RAAS inhibitor (RAASi) treatment explore exact mechanism protective effect. We found that RAASi ameliorate diabetes-induced interstitial decrease profibrotic production. prevents by acting directly on proximal tubular cells,...
Lyophilization is a cost-effective method for biological specimen preservation but detailed tissue-specific reference protocols are still lacking. Moreover, data limited on the long-term stability of proteins and nucleic acids in lyophilized samples.Here, we offer lyophilization various rat mouse tissues (kidney, heart, liver, lung, aorta, skin) coupled with technical hints optimal sample preparation. We demonstrate that samples stored at 4 °C 20 months can yield protein RNA similar quantity...
Introduction Cardiovascular disease (CVD) is two to five times more prevalent in diabetic patients and the leading cause of death. Therefore, identification novel therapeutic strategies that reduce risk CVD a research priority. Clinical trials showed reduction relative heart failure by sodium-glucose cotransporter 2 inhibitors (SGLT2i) are partly beyond their glucose lowering effects, however, molecular mechanisms still elusive. Here we investigated role SGLT2i dapagliflozin (DAPA)...
Mechanisms of renal ischemia-reperfusion injury remain unresolved, and effective therapies are lacking. We previously showed that dehydroepiandrosterone protects against in male rats. Here, we investigated the potential role σ1-receptor activation mediating this protection. In rats, pretreatment with either or fluvoxamine, a high-affinity agonist, improved survival, function structure, inflammatory response after sublethal injury. human proximal tubular epithelial cells, stimulation by...
Diabetic kidney disease is a worldwide epidemic, and therapies are incomplete. Clinical data suggest that improved renal outcomes by Na + -glucose cotransporter 2 inhibitor (SGLT2i) partly beyond their antihyperglycemic effects; however, the mechanisms still elusive. Here, we investigated effect of SGLT2i dapagliflozin (DAPA) in prevention elevated O-GlcNAcylation tubular hypoxia as contributors fibrosis. Type 1 diabetes was induced streptozotocin adult male Wistar rats. After onset...
Increased O-linked β- N-acetylglucosamine glycosylation ( O-GlcNAcylation) is a known contributor to diabetes; however, its relevance in diabetic nephropathy (DN) poorly elucidated. Here, we studied the process and enzymes of O-GlcNAcylation with special emphasis on Akt-endothelial nitric oxide synthase (eNOS) heat shock protein (HSP)72 signaling. Since tubular injury prominent site DN, effect hyperglycemia was first measured proximal (HK2) cells cultured high glucose. In vivo levels...
Previously, we demonstrated gender differences in Na-K-ATPase (NKA) expression and function after renal ischemia-reperfusion (I/R) injury (Sex the alterations of Na(+), K(+)-ATPase following rat kidney. J Physiol 555: 471-480, 2004). Postischemic membrane destruction causes inhibition NKA, whereas heat shock protein (HSP) 72 helps to preserve it. We tested sex postischemic HSP72 colocalization with NKA. The left pedicle uninephrectomized female (F) male (M) Wistar rats was clamped for 55 min...
Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression α smooth muscle actin (α-SMA) that belongs to the family six conserved isoforms showing high degree homology. The aim present study was develop real-time PCRs clearly discriminate α-SMA ß-actin from other isoforms. Real-time using self-designed mouse, human rat specific...