A5035077360- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Renal Transplantation Outcomes and Treatments
- Prenatal Screening and Diagnostics
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Herpesvirus Infections and Treatments
- Synthesis and Biological Evaluation
- Acute Myeloid Leukemia Research
- Cancer Immunotherapy and Biomarkers
- T-cell and Retrovirus Studies
- Polyomavirus and related diseases
- Telomeres, Telomerase, and Senescence
- Liver physiology and pathology
- Advanced Biosensing Techniques and Applications
- Prostate Cancer Treatment and Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Cells and Metastasis
- Calcium signaling and nucleotide metabolism
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
Karolinska Institutet
2013-2025
Academic Center for Dentistry Amsterdam
2022
University of Amsterdam
2022
Vrije Universiteit Amsterdam
2022
Cancer Center Amsterdam
2018-2021
Amsterdam UMC Location Vrije Universiteit Amsterdam
2018-2021
Amsterdam University Medical Centers
2021
University Medical Center
2020
University Hospital and Clinics
2020
Karolinska University Hospital
2013-2017
There is a strong biological rationale for the augmentation of allogeneic natural killer (NK) cell therapies with chimeric antigen receptor (CAR) to enhance acute myeloid leukemia (AML) targeting. CD38 an established immunotherapeutic target in multiple myeloma and under investigation as AML. expression on NK cells its further induction during ex vivo expansion represent barriers development CAR-NK therapy. We set out develop therapy AML, first by using line which has low baseline...
Suppression of immune reactivity by increased expression co-inhibitory receptors has been discussed as a major reason to why the system fails control tumor development. Elucidating pattern tumor-infiltrating lymphocytes in different cancer types will help develop future treatment strategies. We characterized markers reflecting and affecting T-cell functionality flow cytometry on isolated from blood, ascites advanced ovarian patients (n = 35). Significantly higher proportions CD4+ CD8+...
Long-term stable mixed chimerism (MC) is a rare phenomenon after hematopoietic stem cell transplantation (HSCT) characterized by 5% to 95% residual recipient cells. The underlying mechanisms of MC are largely unknown. In this study we compared full donor with long-lasting for median 9.5 years (range, 5 16.5) post-HSCT in patients nonmalignant diseases. Several factors significantly associated the likelihood development were identified univariate analysis, eg, younger age, sibling donor, and...
Abstract Objectives The clinical outcome after allogeneic haematopoietic stem cell transplantation (aHCT) relies greatly on the efficient recovery of T cells. Several studies have investigated short‐term γδ reconstitution and their role in outcomes following transplantation. Nevertheless, long‐term characteristics impact remained largely unknown. Methods We analysed cells from 20 recipient/donor pairs at phenotypic, clonotypic functional levels to assess ≥ 8 years (median 18 years)...
Chronic graft-versus-host disease (cGVHD) is a debilitating complication arising in around half of all patients treated with an allogeneic hematopoietic stem cell transplantation. Even though treatment severe cGVHD has improved during recent years, it remains one the main causes morbidity and mortality affected patients. Biomarkers blood that could aid diagnosis classification severity are needed for development novel strategies can alleviate symptoms reduce need painful sometimes...
Chimeric antigen receptor (CAR) T cells are highly successful in the treatment of hematologic malignancies. We recently generated affinity-optimized CD38CAR cells, which effectively eliminate multiple myeloma (MM) with little or no toxicities against nonmalignant hematopoietic cells. The lack universal donors and long manufacturing times however limit broad application CAR cell therapies. Natural killer (NK) from third party individuals may represent a viable source “off shelf” CAR-based...
Long-term stable mixed chimerism is a rare and poorly understood phenomenon post hematopoietic stem cell transplantation. This study aims to shed light on whether the two systems in patients with remain functional. Additionally, we investigate possible immunologic differences these individuals compared only donor derived immune cells. Patients chimerism, at median 10 (5–16) years post-HSCT for non-malignant diseases, were assessed regarding clinical situation system (phenotypical...
We have recently shown the strong negative impact of multiple myeloma (MM)-bone marrow mesenchymal stromal cell (BMMSC) interactions to several immunotherapeutic strategies including conventional T cells, chimeric antigen receptor (CAR) and daratumumab-redirected NK cells. This BMMSC-mediated immune resistance via upregulation antiapoptotic proteins in MM cells was mainly observed for moderately cytotoxic modalities. Here, we set out assess hypothesis that this distinct mode evasion can be...
Acute graft-versus-host disease (aGVHD) is 1 of the main major complications post-hematopoietic stem cell transplantation (HSCT). Identifying patients at risk severe aGVHD may lead to earlier intervention and treatment, resulting in increased survival a better quality life. We aimed identify biomarkers donor grafts patient plasma around time that might be predictive development. build on our previously published methods by using multiplex assays multicolor flow cytometry. identified 5 easily...
Background . Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). We designed functional assay for assessment of individual risk acute GVHD. Study Design and Methods Blood samples were collected from patients donors before HSCT. Two groups seven each selected, one in which individuals developed GVHD grades II–IV none showed any clinical signs Peripheral blood mononuclear cells (PBMCs) isolated incubated mixed lymphocyte...
Less than a third of patients with acute myeloid leukemia (AML) are cured by chemotherapy and/or hematopoietic stem cell transplantation, highlighting the need to develop more efficient drugs. The low efficacy standard treatments is associated inadequate depletion CD34+ blasts and leukemic cells, latter drug-resistant subpopulation cells characterized CD34+CD38- phenotype. To target these primitive better, we have designed CD34/CD3 bi-specific T-cell engager (BTE) its anti-leukemia potential...
BACKGROUND Benign prostatic hyperplasia (BPH) is the most common benign adenoma and prostate cancer frequent malignancy in men over 50 years of age Western world, where it remains a significant health problem. Prostate lesions are known to contain immune cells, which may contribute control tumor progression. However, due their low numbers restricted access necessary material difficult isolate cells from tissue characterize immunological features. METHODS An efficient robust method was...
Abstract This study investigated how stromal cells affect the IL-2 pathway in alloantigen-activated T cells. We found that decidual (DSCs) from term placentas promoted a high production of cultures with The intensity expression cluster differentiation 25 (CD25; IL-2Rα) on was increased by DSCs, whereas frequency and signaling subunits CD122 (IL-2Rβ) CD132 (IL-2Rγc) were reduced. Consequently, uptake STAT5 phosphorylation (pSTAT5) abrogated. DSCs also decreased proportion pSTAT5+ response to...
Immune reconstitution is often impaired for months to years in patients after allogeneic hematopoietic stem cell transplantation (HSCT) which predisposes them a wide collection of clinical infections.
Despite the considerable progress in acute myeloid leukemia (AML) treatment, relapse after allogeneic hematopoietic stem cell transplantation (HSCT) is still frequent and associated with a poor prognosis. Relapse has been shown to be correlated an incomplete eradication of CD34+ leukemic cells prior HSCT. Previously, we have that novel CD34-directed, bispecific T-cell engager (BTE) can efficiently redirect effector function toward cancer cells, thus eliminating vitro vivo. However, its...
Allogeneic hematopoietic Stem Cell Transplantation (ASCT) is well established as a curative treatment for many hematological malignancies and non-malignant disorders.The aim of ASCT in these diseases to achieve sustained donor engraftment fight leukemic cells malignant disease, improve function, provide immune competence or normalize enzyme deficiency.Peripheral blood bone marrow commonly used monitor after ASCT.The presence mixed donor/recipient chimerism transplantation, donor/donor double...