A5071021225- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced biosensing and bioanalysis techniques
- Biochemical effects in animals
- CRISPR and Genetic Engineering
- Estrogen and related hormone effects
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Protein Hydrolysis and Bioactive Peptides
- Stress Responses and Cortisol
- Photochromic and Fluorescence Chemistry
- Neuroendocrine regulation and behavior
- Insect Utilization and Effects
- Ion channel regulation and function
- Food Industry and Aquatic Biology
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
Veneto Institute of Molecular Medicine
2012-2024
Neuroscience Institute
2015-2024
University of Padua
2012-2024
National Research Council
2020-2023
Giovanni Armenise-Harvard Foundation
2019
Institute of Molecular Medicine
2019
Consorzio Roma Ricerche
2011
Columbia University
2007
Duke Medical Center
2002-2003
Duke University Hospital
2002-2003
The evolving field of food technology is increasingly dedicated to developing functional foods. This study explored bioactive peptides from sunflower protein isolate (SPI), obtained defatted flour, a by-product the oil processing industry. SPI underwent simulated gastrointestinal digestion and peptide-enriched fraction (PEF) showed antioxidant properties in vivo, zebrafish. Among present PEF identified by mass spectrometry analysis, we selected those with silico evaluation, considering their...
The mesencephalic dopaminergic (mDA) cell system is composed of two major groups projecting cells in the Substantia Nigra (SN) (A9 neurons) and Ventral Tegmental Area (VTA) (A10 cells). Selective degeneration A9 neurons occurs Parkinson's disease (PD) while abnormal function A10 has been linked to schizophrenia, attention deficit addiction. molecular basis that underlies selective vulnerability presently unknown. By taking advantage transgenic labeling, laser capture microdissection coupled...
The type of neuronal activity required for circuit development is a matter significant debate. We addressed this issue by analyzing the topographic organization olfactory bulb in transgenic mice engineered to have very little afferent spontaneous due overexpression inwardly rectifying potassium channel Kir2.1 sensory neurons (Kir2.1 mice). In these conditions, topography was unrefined. Odor-evoked responses were readily recorded glomeruli with reduced activity, although functional maps...
Optical recordings of neuronal activity at cellular resolution represent an invaluable tool to investigate brain mechanisms. Zebrafish larvae is one the few model organisms where, using fluorescence-based reporters cell activity, it possible optically reconstruct dynamics across whole brain. Typically, leveraging reduced light scattering, methods like lightsheet, structured illumination, and light-field microscopy use spatially extended excitation profiles detect in parallel signals from...
Neurotrophins are important regulators of visual cortical plasticity. It is still unclear, however, whether they play similar or different roles and which their effects on the electrical activity neurons in vivo. We therefore compared all neurotrophins, nerve growth factor (NGF), brain-derived neurotrophic (BDNF), neurotrophin-4 (NT-4), neurotrophin-3 (NT-3) plasticity cell spontaneous visually evoked activity. Rats were monocularly deprived for 1 week at peak critical period, neurotrophins...
A distinctive feature in the topographic organization of olfactory system mammals is dual function odorant receptor (OR): it detects odors nasal epithelium and plays an instructive role axonal convergence sensory neurons (OSN) into bulb (OB). The latter supported by genetic experiments expression OR not only on cilia, but also axon termini OSN. signaling pathway coupled to cilia well known recognized involve cAMP Ca(2+), whereas, until now, nothing was functional characteristics...
The mechanism of cGMP production in olfactory sensory neurons (OSNs) is poorly understood, although this messenger takes part several key processes such as adaptation, neuronal development, and long-term cellular responses to odorant stimulation. Many aspects the regulation OSNs are still unknown or highly controversial, its subcellular heterogeneity, coupling receptors downstream targets. Here, we have investigated dynamics intracellular distribution living rat culture transfected with a...
Highlights•Axonal odorant receptors respond to cues elaborated in the olfactory bulb•PEBP1, expressed bulb, is a putative ligand of axonal receptors•Genetic ablation PEBP1 results disrupted map vivo•Axonal modulate axon targeting sensory formationSummaryIn mammals, not only detect odors but also define target where neurons project give rise map. The receptor at cilia, it binds odorants, and terminal. mechanism activation function terminal is, however, still unknown. Here, we identify...
NanoChlor, a nanoparticle-based fluorescent probe for chloride that is both ratiometric and capable of spontaneously penetrating neuronal cells at submillimolar concentrations, was designed studied. NanoChlor built on silica nanoparticles grafted with 6-methoxyquinolinium as the chloride-sensitive component fluorescein reference dye. A Stern–Volmer constant 50 M–1 measured in Ringer's buffer pH 7.2, response to chemically induced currents recorded real time hippocampal cells.
Intimate partner violence (IPV) is a significant public health concern whose neurological/behavioral sequelae remain to be mechanistically explained. Using mouse model recapitulating an IPV scenario, we evaluated the female brain neuroendocrine alterations produced by reiterated male-to-female violent interaction (RMFVI). RMFVI prompted anxiety-like behavior in mice hippocampus displayed marked neuronal loss and hampered neurogenesis, namely reduced BrdU-DCX-positive nuclei diminished...
Abstract It is known that administration of nerve growth factor (NGF) prevents the ocular dominance shift induced by monocular deprivation in rat. To determine whether electrical activity visual afferent pathway required for NGF effects on dominance, we infused into cortex animals subjected to complete blockade retinal discharges. Rats at peak critical period received intravitreal tetrodotoxin (TTX) injections silence one eye a 6–7 days; was concurrently delivered means osmotic minipumps. At...
Abstract We have explored whether overexpression of the bcl‐2 gene ‘per se’ can promote regeneration retinal ganglion cells (RGCs) after optic nerve axotomy in developing transgenic mice. used newborn mice (postnatal day 5) because at this age central nervous system environment is more permissive for than adults, thus, maximizing probability to detect a regeneration‐promoting role . Thirty days postsurgery we found that overexpressing , high proportion survived and also some fibers proximal...
Among the X-linked genes associated with intellectual disability, Oligophrenin-1 (OPHN1) encodes for a Rho GTPase-activating protein, key regulator of several developmental processes, such as dendrite and spine formation synaptic activity. Inhibitory interneurons play role in development function neuronal circuits. Whether mutation OPHN1 can affect morphology properties inhibitory remains poorly understood. To address these open questions, we studied well-established mouse model i.e. line...
Abstract Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present monogenic diseases of the central nervous system caused by random inactivation X-chromosome, case X-linked pathologies, or somatic mutations affecting subset neurons. The comprehension mechanisms these and cell-autonomous effects specific requires generation sparse mosaic models, genotype each neuron univocally identified expression fluorescent protein vivo. Here, we show...
Significance Intellectual disability associated with Oligophrenin 1 (OPHN1) mutation is a neurodevelopmental disorder characterized by wide range of cognitive and social dysfunction. Here, we dissected the impact OPHN1 on postnatal migration inhibitory interneurons, process that thought to play key role in developmental plasticity. We found loss function led an early maturation morphofunctional properties neuronal precursors impaired their resulted dramatically lower number cells reaching...