A5070352001- Autophagy in Disease and Therapy
- Immune Cell Function and Interaction
- Skin and Cellular Biology Research
- Hair Growth and Disorders
- T-cell and B-cell Immunology
- Skin Protection and Aging
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- melanin and skin pigmentation
- Dermatologic Treatments and Research
- Dermatology and Skin Diseases
- Influenza Virus Research Studies
- Lipid metabolism and biosynthesis
- Cytomegalovirus and herpesvirus research
- Wound Healing and Treatments
- Sirtuins and Resveratrol in Medicine
- Genomics, phytochemicals, and oxidative stress
- Cannabis and Cannabinoid Research
- Toxin Mechanisms and Immunotoxins
- Cellular Mechanics and Interactions
- Cell death mechanisms and regulation
- Healthcare and Venom Research
- Virus-based gene therapy research
Medical University of Vienna
2011-2023
SKiN Health
2014
In-Q-Tel
2005
Brigham and Women's Hospital
1994-2000
Harvard University
1994-1997
Forschungs- und Beratungsstelle Arbeitswelt
1981-1985
Memory T lymphocytes extravasate at sites of inflammation, but the mechanisms employed by these cells to initiate contact and tethering with endothelium are incompletely understood. An important part leukocyte extravasation is initiation rolling adhesions on endothelial selectins; such events have been studied in monocytes neutrophils not lymphocytes. In this study, potential adhere roll selectins vitro was investigated. We demonstrate that can form tethers P selectin E under physiologic...
Abstract The angiogenic cytokine vascular endothelial growth factor (VEGF)-A plays a central role in both wound healing and tumor growth. In the skin, epidermal keratinocytes are major source of this factor. To study contribution keratinocyte-derived VEGF-A to these angiogenesis-dependent processes, we generated mice which was inactivated specifically keratin 5-expressing tissues. mutant were macroscopically normal, skin capillary system well established, demonstrating that is not essential...
Cutaneous lymphocyte antigen (CLA), defined by the HECA-452 antibody, is a cell surface glycoprotein found on subset of T cells in peripheral blood that binds specifically to E-selectin. This marker present majority at sites cutaneous inflammation and immune responses. Based upon such evidence, an association between CLA expression skin homing has been proposed. To understand better this relationship, we asked whether putative disease-related, antigen-specific expressed CLA. In study,...
Abstract Both IL-1 alpha and beta lack an N terminus secretory sequence, the mechanism of secretion these pleiotropic cytokines is incompletely understood. The epidermis contains large quantities in keratinocytes, which may play a role inducing endothelial adhesion molecules promoting extravasation leukocytes. Here we report that mechanical deformation human keratinocytes leads to rapid release alpha, possibly through transient disruptions plasma membrane. Using device precisely controls...
To investigate the role of angiogenic cytokine vascular endothelial growth factor (VEGF) during pregnancy and lactation, we used mice in which VEGF had been inactivated mammary gland epithelial cells. Pups born to mutant mothers failed thrive, displaying little milk their stomachs. However, when they were transferred control developed normally. Investigation morphology revealed that lobulo-alveolar expansion into fat pad was not complete lactating glands, an accumulation globules evident...
The thymic epithelium plays critical roles in the positive and negative selection of T cells. Recently, it was proposed that autophagy epithelial cells is essential for induction cell tolerance to self antigens thus prevention autoimmune diseases. Here we have tested this hypothesis using mouse models which blocked specifically expressing keratin 14 (K14), including precursor While mice carrying floxed Atg7 gene (ATG7 f/f) showed a high level autophagy, as determined by LC3 Western blot...
We have reported recently that inactivation of the essential autophagy-related gene 7 (Atg7) in keratinocytes has little or no impact on morphology and function epidermal barrier experimental animals. When these mice aged, mutant males, (Atg7 ΔKC), developed an oily coat. As keratin 14 promoter driven cre/LoxP system inactivates floxed Atg7 all (K14) expressing cells, including sebocytes, we investigated whether hair phenotype was consequence changes skin sebaceous glands. Using antibody to...
Induction of cross-protective immunity against serologically distinct subtypes influenza A virus in mice was examined an attempt to correlate cross-protection with heterotypic lymphocyte responses. Live and inactivated vaccines protected the homologous subtype, but only whole heterologous subtypes. provided better than vaccines. weak defense challenge generated by live H0N1 could be boosted cross-stimulation H3N2 restimulation pathogenic virus. Heterotypic protection persisted for at least...
The epithelial derived Harderian gland consists of 2 types secretory cells. more numerous type A cells are responsible for the secretion lipid droplets, while B produce dark granules multilamellar bodies. process autophagy is constitutively active in gland, as confirmed by our analysis LC3 processing GFP-LC3 transgenic mice. This compromised deletion Atg7. Morphologically, Atg7 mutant glands hypotrophic and degenerated, with highly vacuolated pyknotic nuclei. accumulate droplets coated PLIN2...
Targeted gene knockout mouse models have helped to identify roles of autophagy in many tissues. Here, we investigated the retinal pigment epithelium (RPE) Atg7f/f Tyr-Cre mice (on a C57BL/6 background), which Cre recombinase is expressed under control tyrosinase promoter delete Atg7. In line with cell-directed blockade autophagy, RPE and melanocytes choroid showed strong accumulation adaptor substrate, sequestosome 1 (Sqstm1)/p62, relative levels mice. Immunofluorescence Western blot...