A5081219190- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Healthcare and Venom Research
- Marine Invertebrate Physiology and Ecology
- Antimicrobial Peptides and Activities
- Science and Education Research
- Menopause: Health Impacts and Treatments
- Estrogen and related hormone effects
- Blood Coagulation and Thrombosis Mechanisms
- Fish biology, ecology, and behavior
- Neurotransmitter Receptor Influence on Behavior
- Medicinal Plants and Neuroprotection
- Meat and Animal Product Quality
- Alkaloids: synthesis and pharmacology
- Neurobiology and Insect Physiology Research
- Tryptophan and brain disorders
- Calpain Protease Function and Regulation
- Gender, Sexuality, and Education
- Public Health in Brazil
- Leech Biology and Applications
- Bee Products Chemical Analysis
Instituto Butantan
2012-2024
Laboratoire de Chimie
2000
Scorpion venom is a mixture of peptides, including antimicrobial, bradykinin-potentiating and anionic peptides small to medium proteins, such as ion channel toxins, metalloproteinases phospholipases that together cause severe clinical manifestation. Tityus bahiensis the second most medically important scorpion species in Brazil it widely distributed country with exception North Region. Here we sequenced analyzed transcripts from glands T. bahiensis, aiming at identifying annotating gland...
Background Except for the northern region, where Amazonian black scorpion, T. obscurus, represents predominant and most medically relevant scorpion species, Tityus serrulatus, Brazilian yellow is widely distributed throughout Brazil, causing envenoming fatalities due to sting. In order evaluate compare diversity of venom components obscurus we performed a transcriptomic investigation telsons (venom glands) corroborated by shotgun proteomic analysis from two species. Results The putative...
A great number of studies on scorpion venoms associate their effects to the autonomic nervous system, and few data are available about action central system (CNS). The aim this work was evaluate some after intraperitoneal injection Tityus serrulatus or T. bahiensis venoms. hippocampal concentration neurotransmitters metabolites were determined. Electroencephalographic behavioral observations performed, all brains removed for histopathological analysis areas. Both induced electrographic...
There are a great number of studies about Brazilian scorpions. However, little is known the venom scorpions northern Brazil, mainly Tityus obscurus, which responsible for most accidents in Amazon. Thus, this study aimed to evaluate some pharmacological effects T. obscurus rats and mice. In rats, (10 mg/kg i.p.) caused hemorrhagic patches lung parenchyma but did not lead pulmonary edema. was decrease general activity, observed activity box after injection. The induce changes occurrence...
The objective of the present study was to determine effects a toxin from T. bahiensis scorpion venom on hippocampus rats. This toxin, called Tb V-4, chosen since it shows remarkable convulsive activity.Male Wistar rats weighing 250g were used. (1.0μg/μl) injected into hippocampus. animals then submitted electroencephalographic and behavioral examinations or microdialysis levels neurotransmitters. location implanted guide cannulae electrodes checked histologically. number cells in CA1, CA3...
Scorpion envenoming is a public health problem in Brazil, where Tityus serrulatus and T. bahiensis are considered the most dangerous scorpions. They well adapted to urbanized environments, there an increasing probability of human exposure these venoms, including during pregnancy. Not much known about effects prenatal venom, no information available aid rational treatment victims stung Thus, this study aimed investigate whether venom from scorpion administered once pregnant female rats at...
Scorpion venoms contain low molecular weight basic polypeptides, neurotoxins, that are the principal toxic agents. These toxins act on ion channels, promoting a derangement may result in an abnormal release of neurotransmitters. In present study we investigated some effects F, H and J fractions isolated from Tityus serrulatus scorpion venom central nervous system rodents. The was partially purified by gel filtration chromatography. neurotoxic effect these studied convulsive activity after...
Tityus serrulatus is the most venomous scorpion in Brazil; however, it not known whether its venom causes any harm to offspring whose mothers have received it. This study investigates of T. may lead deleterious effects offspring, when once administered pregnant rats at a dose that moderate envenomation (3mg/kg). The were studied on 5th and 10th gestation day (GD5 GD10). maternal reproductive parameters group GD5 showed no alteration. GD10 presented an increase post-implantation losses. In...
Scorpion venoms are composed of several substances with different pharmacological activities. Neurotoxins exert their effects by targeting ion channels resulting in toxic to mammals, insects and crustaceans. Tb II-I, a fraction isolated from Tityus bahiensis scorpion venom, was investigated for its ability induce neurological immune-inflammatory effects. Two putative β-sodium channel toxins were identified this fraction, Tb2 II 4, the latter having been completely sequenced mass...
Scorpion venoms are a rich source of bioactive peptides, most which neurotoxic, with 30 to 70 amino acid residues in their sequences. There scarcity reports the literature concerning short linear peptides found scorpion venoms. This type peptide toxin may be selectively extracted from venom using 50% (v/v) acetonitrile. The use LC-MS and MS/MS enabled detection 12 six were identified as cryptides. These shown multifunctional, causing hemolysis, mast cell degranulation lysis, edema, pain,...
TsTX-I scorpion toxin, also known as γ-toxin, is a β-toxin which binds to site 4 of the sodium channel, shifting its activation potential. There are few studies about pharmacological action in central nervous system. The objective this work was determine electroencephalographic, behavioral and histopathological effects intrahippocampal injection TsTX-I. Rats were anesthetized fitted with cannulae for into hippocampus electrodes cerebral recording. animals treated Ringer solution, some doses...