A5074138625- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- Algal biology and biofuel production
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Mitochondrial Function and Pathology
- Microbial infections and disease research
- Plant biochemistry and biosynthesis
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Salmonella and Campylobacter epidemiology
- Porphyrin Metabolism and Disorders
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Biochemical and biochemical processes
- Phytoplasmas and Hemiptera pathogens
- Biochemical and Structural Characterization
- Fuel Cells and Related Materials
- Endoplasmic Reticulum Stress and Disease
- Surface Chemistry and Catalysis
University of Liverpool
2017-2024
Systems Biology Institute
2024
Protein nanocages have emerged as promising candidates for enzyme immobilization and cargo delivery in biotechnology nanotechnology. Carboxysomes are natural proteinaceous organelles cyanobacteria proteobacteria exhibited great potential creating versatile a wide range of applications given their intrinsic characteristics self-assembly, encapsulation, permeability, modularity. However, how to program intact carboxysome shells with specific docking sites tunable efficient loading is key...
Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. The carboxysome specialized bacterial organelle that encapsulates enzymes into virus-like protein shell plays essential roles photosynthetic carbon fixation. naturally designed architecture, semi-permeability, catalytic improvement carboxysomes have inspired rational design engineering new nanomaterials to incorporate desired the for enhanced performance. Here, we...
Carboxysomes are proteinaceous organelles that play essential roles in enhancing carbon fixation cyanobacteria and some proteobacteria. These self-assembling encapsulate Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) carbonic anhydrase using a protein shell structurally resembling an icosahedral viral capsid. The serves as physical barrier to protect enzymes from the cytosol selectively permeable membrane mediate transport of enzyme substrates products. structural mechanical...
How thylakoid membranes are generated to form a metabolically active membrane network and how orchestrate the insertion localization of protein complexes for efficient electron flux remain elusive. Here, we develop method modulate biogenesis in rod-shaped cyanobacterium Synechococcus elongatus PCC 7942 by modulating light intensity during cell growth, probe spatial-temporal stepwise process cells. Our results reveal that plasma regularly arranged concentric layers have no physical...
Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation cyanobacteria and some chemoautotrophs. This self-assembling organelle encapsulates the key CO2-fixing enzymes, Rubisco, carbonic anhydrase using a polyhedral protein shell is constructed by hundreds of paralogs. The α-carboxysome from chemoautotroph Halothiobacillus neapolitanus serves as model system fundamental studies synthetic engineering carboxysomes. In this study, we adopted...
Abstract The growth in world population, climate change, and resource scarcity necessitate a sustainable increase crop productivity. Photosynthesis major crops is limited by the inefficiency of key CO 2 -fixing enzyme Rubisco, owing to its low carboxylation rate poor ability discriminate between O . In cyanobacteria proteobacteria, carboxysomes function as central organelles that elevate levels around encapsulated Rubisco enhance carboxylation. There growing interest engineering into...
Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous with faster anticipated enhance crop photosynthesis and productivity. requirement chaperones for expression assembly has obstructed efficient production functional foreign in chloroplasts. Here, we report...
Abstract Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role carboxysome assembly Rubisco encapsulation. Its mechanism action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components determine cryoEM structures these decipher principle The...
Carboxysomes are proteinaceous bacterial microcompartments that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating several enzymes, including ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible first step Calvin-Benson-Bassham cycle. Despite their significance great bioengineering potentials, structural understanding native carboxysomes is currently limited to low-resolution...
Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla and provide a means for compartmentalizing specific metabolic pathways. They sequester catalytic enzymes from the cytoplasm, using an icosahedral shell with selective permeability to molecules substrates, enhance efficiency. Carboxysomes were first BMCs discovered their unprecedented capacity of CO2 fixation allows cyanobacteria make significant contribution global carbon fixation. There is...
Carboxysomes are membrane-free organelles for carbon assimilation in cyanobacteria. The carboxysome consists of a proteinaceous shell that structurally resembles virus capsids and internal enzymes including ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), the primary carbon-fixing enzyme photosynthesis. formation carboxysomes requires hierarchical self-assembly thousands protein subunits, initiated from Rubisco assembly packaging to encapsulation. Here we study role factor 1 (Raf1)...
The carboxysome is a versatile paradigm of prokaryotic organelles and proteinaceous self-assembling microcompartment that plays essential roles in carbon fixation all cyanobacteria some chemoautotrophs. encapsulates the central CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), using polyhedral protein shell selectively permeable to specific metabolites favor Rubisco carboxylation. There tremendous interest repurposing carboxysomes boost heterologous organisms....
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. Following optimization logistics that shipping isolates as thermolysates in ambient conditions, project assembled diverse 10,419 from low- middle-income countries. The genomes were sequenced using LITE library...
Rubisco is the essential enzyme mediating fixation of atmospheric CO2 during photosynthesis. In cyanobacteria, enzymes are densely packed and encapsulated in a specialized organelle known as carboxysome. Well-defined assembly carboxysome formation pivotal for efficient fixation. Numerous chaperone proteins, including RbcX, proper protein folding assembly. this study, we investigated vivo function RbcX cyanobacterium Synechococcus elongatus PCC 7942 (Syn7942) using molecular, biochemical,...
Abstract Bacterial metabolosomes are a family of protein organelles in bacteria. Elucidating how thousands proteins self-assemble to form functional is essential for understanding their significance cellular metabolism and pathogenesis. Here we investigate the de novo biogenesis propanediol-utilization (Pdu) characterize roles key constituents generation intracellular positioning metabolosomes. Our results demonstrate that Pdu metabolosome undertakes both “Shell first” “Cargo assembly...
The carboxysome is a protein-based nanoscale organelle in cyanobacteria and many proteobacteria, which encapsulates the key CO2-fixing enzymes ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carbonic anhydrase (CA) within polyhedral protein shell. intrinsic self-assembly architectural features of carboxysomes semipermeability shell provide foundation for accumulation CO2 enhanced carboxylation. Here, we develop an approach to determine interior pH conditions inorganic carbon...
Abstract Many pathogenic bacteria use proteinaceous ethanolamine-utilization microcompartments (Eut BMCs) to facilitate the catabolism of ethanolamine, an abundant nutrient in mammalian gut. The ability metabolize ethanolamine gives pathogens a competitive edge over commensal microbiota which can drive virulence inflamed Despite their critical functions, molecular mechanisms underlying synthesis Eut BMCs bacterial cells remain elusive. Here, we report systematic study for dissecting basis...
Abstract Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation cyanobacteria. This self-assembling proteinaceous organelle encapsulates the key CO 2 -fixing enzymes, Rubisco and carbonic anhydrase, using a polyhedral shell constructed by hundreds of protein paralogs. Deciphering precise arrangement structural organization enzymes within carboxysomes is crucial for understanding formation process overall functionality carboxysomes. Here, we...
The human skin microbiome comprises diverse populations that differ temporally between body sites and individuals. virome is a less studied component of the study bacteriophages required to increase knowledge modulation stability bacterial communities. Staphylococcus species are among most abundant colonisers associated with both health disease yet infecting on well studied. Here, we report isolation genome sequencing 40 from swabs infect coagulase-negative (CoNS) species, which extends our...
Carboxysomes are the specific CO2-fixing microcompartments in all cyanobacteria. Although it is known that organization and subcellular localization of carboxysomes dependent on external light conditions highly relevant to their functions, how carboxysome function actively orchestrated natural diurnal cycles has remained elusive. Here, we explore dynamic regulation positioning carbon fixation model cyanobacterium Synechococcus elongatus PCC 7942 response light-dark cycles, using live-cell...
<title>Abstract</title> Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) is the central enzyme for converting atmospheric CO2 into organic molecules, playing a crucial role in global carbon cycle. In cyanobacteria and some chemoautotrophs, complexes, along with carbonic anhydrase, are enclosed within specific proteinaceous microcompartments, known as carboxysomes. The polyhedral carboxysome shell ensures dense packaging of creates high-CO2 internal environment to facilitate fixation...
Abstract Carboxysomes are anabolic bacterial microcompartments that play an essential role in CO2 fixation cyanobacteria. This self-assembling proteinaceous organelle uses a polyhedral shell constructed by hundreds of protein paralogs to encapsulate the key CO2-fixing enzymes Rubisco and carbonic anhydrase. Deciphering precise arrangement structural organization within carboxysomes is crucial for understanding carboxysome formation overall functionality. Here, we employed cryoelectron...
Abstract Carboxysomes are proteaceous bacterial microcompartments (BMCs) that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating carbonic anhydrase ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), which catalyses dehydration bicarbonate into CO 2 , first step Calvin–Benson–Bassham cycle. Despite their significance great bioengineering potentials, structural characterization native...
Abstract Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role carboxysome assembly Rubisco encapsulation. Its mechanism action, however, is not fully understood. Here we synthetically engineered α-carboxysome shells using minimal shell components determined cryoEM structures these decipher principle...