A5070093882- Escherichia coli research studies
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Urinary Tract Infections Management
- Probiotics and Fermented Foods
- Urinary Bladder and Prostate Research
- Viral gastroenteritis research and epidemiology
- Ruminant Nutrition and Digestive Physiology
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
- Veterinary Equine Medical Research
- Transgenic Plants and Applications
- Pelvic floor disorders treatments
- Antimicrobial agents and applications
- MicroRNA in disease regulation
- Garlic and Onion Studies
- Antimicrobial Peptides and Activities
- Organometallic Compounds Synthesis and Characterization
- IL-33, ST2, and ILC Pathways
- Women's cancer prevention and management
- CRISPR and Genetic Engineering
Universidade Estadual Paulista (Unesp)
2016-2025
Louisiana State University
2023-2025
Universidad de la República de Uruguay
2023
Centro Universitário de Araraquara
2019-2020
Instituto de Ciências Farmacêuticas
2017
The University of Texas Southwestern Medical Center
2006-2016
Universidade de São Paulo
2006-2016
Southwestern Medical Center
2008-2014
Emory University
2010
Brigham and Women's Hospital
2008
Many bacterial pathogens rely on a conserved membrane histidine sensor kinase, QseC, to respond host adrenergic signaling molecules and signals in order promote the expression of virulence factors. Using high-throughput screen, we identified small molecule, LED209, that inhibits binding preventing its autophosphorylation consequently inhibiting QseC-mediated activation gene expression. LED209 is not toxic does inhibit pathogen growth; however, this compound markedly several vitro vivo...
The autoinducer-3 (AI-3)/epinephrine (Epi)/norepinephrine (NE) interkingdom signaling system mediates chemical communication between bacteria and their mammalian hosts. three signals are sensed by the QseC histidine kinase (HK) sensor. Salmonella enterica serovar Typhimurium is a pathogen that uses HKs to sense its environment regulate virulence. invades epithelial cells survives within macrophages. Invasion of mediated type III secretion (T3SS) encoded in pathogenicity island 1 (SPI-1),...
Gastrointestinal (GI) bacteria sense diverse environmental signals as cues for differential gene regulation and niche adaptation. Pathogens such enterohemorrhagic Escherichia coli (EHEC), which causes bloody diarrhea, use these the temporal energy-efficient of their virulence factors. One main strategies employed by EHEC is formation attaching effacing (AE) lesions on enterocytes. Most genes necessary are grouped within a pathogenicity island, locus enterocyte effacement (LEE), whose...
Invasive pathogens interface with the host and its resident microbiota through interkingdom signaling. The bacterial receptor QseC, which is a membrane-bound histidine sensor kinase, responds to stress hormones epinephrine norepinephrine signal AI-3, integrating signaling at biochemical level. Importantly, QseC cascade exploited by many promote virulence. Here, we translated this basic science information into development of potent small molecule inhibitor LED209. Extensive structure...
Recurrent urinary tract infections (RUTI) are prevalent and pose significant clinical challenges. Although the term RUTI has long been vaguely defined, a consensus definition emerged in recent years. The exact etiology behind remains under debate, with valid arguments for both ascending reinfections as well persistent infection inside bladder. These exist form of quiescent intracellular reservoirs mouse model may represent novel concept to explain UTI recurrence humans. Manageable risk...
ABSTRACT Enteric pathogens such as enterohemorrhagic Escherichia coli (EHEC) and Citrobacter rodentium , which is largely used a surrogate EHEC model for murine infections, are exposed to several host neurotransmitters in the gut. An important chemical exchange within gut involves epinephrine and/or norepinephrine, extensively reported increase virulence gene expression EHEC, acting through two bacterial adrenergic sensors: QseC QseE. However, unable establish itself cause its hallmark...
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. Following optimization logistics that shipping isolates as thermolysates in ambient conditions, project assembled diverse 10,419 from low- middle-income countries. The genomes were sequenced using LITE library...
The bacterial adrenergic sensor kinases QseC and QseE respond to epinephrine and/or norepinephrine initiate a complex phosphorelay regulatory cascade that modulates virulence gene expression in several pathogens. We have previously shown activates Salmonella enterica serovar Typhimurium. Here we report the role of S. Typhimurium pathogenesis as well interplay between these two histidine regulation. An qseE mutant is hampered invasion epithelial cells intramacrophage replication. ΔqseC strain...
ABSTRACT The mouse pathogen Citrobacter rodentium is utilized as a model organism for studying infections caused by the human pathogens enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. (EHEC) to elucidate mechanisms of mucosal immunity. In response C. infection, innate lymphoid cells T secrete interleukin (IL)-22, cytokine that promotes barrier function. IL-22 plays pivotal role in enabling mice survive recover from although exact involved remain incompletely understood....
ABSTRACT Microcolony formation is one of the initial steps in biofilm development, and enteropathogenic Escherichia coli (EPEC) it mediated by several adhesins, including bundle-forming pilus (BFP) EspA filament. Here we report that EPEC forms biofilms on plastic under static conditions a flowthrough continuous culture system. The abilities isogenic mutants to form were assessed. Adhesins such as BFP EspA, important microcolony epithelial cells, are also involved bacterial aggregation during...
Gram-negative bacteria have an outer membrane containing LPS. LPS is constituted of oligosaccharide portion and a lipid-A moiety that embeds this molecule within the membrane. pathogen-associated molecular pattern, several pathogens modify their as stealth strategy to avoid recognition by innate immune system gain resistance host factors disrupt bacterial cell envelope. An essential feature Salmonella enterica Typhimurium pathogenesis its ability replicate vacuoles in professional...
Helicobacter pylori inhabits the gastric epithelium and can promote development of disorders, such as peptic ulcers, acute chronic gastritis, mucosal lymphoid tissue (MALT), adenocarcinomas. To use nanotechnology a tool to increase antibacterial activity silver I [Ag(I)] compounds, this study suggests new strategy for H. infections, which have hitherto been difficult control. [Ag (PhTSC·HCl)2] (NO3)·H2O (compound 1) was synthesized, characterized, loaded into polymeric nanoparticles (PN1)....
Enteroaggregative Escherichia coli (EAEC) is distinguished by its characteristic aggregative adherence (AA) pattern to cultured epithelial cells. In this study we investigated the role of type I fimbriae (TIF) in AA HEp-2 cells and biofilm formation. Accentuation was observed when assay performed absence mannose. This effect prototype EAEC strain 042 (O44:H18), O128:H35 strains for other serotypes. Antiserum against TIF decreased 70% 90% 18 (O128:H35 strain), respectively. A non-polar...
Proteus mirabilis is an important cause of urinary tract infection (UTI) in patients with complicated tracts. Thirty-five strains P. isolated from UTI were examined for the adherence capacity to epithelial cells. All isolates displayed aggregative (AA) HEp-2 cells, a phenotype similarly presented LLC-MK(2) Biofilm formation on polystyrene was also observed all strains. The mannose-resistant Proteus-like fimbriae (MR/P), Type I and AAF/I, II III enteroaggregative Escherichia coli searched by...
Enteroaggregative Escherichia coli (EAEC) is an emerging cause of diarrhea in both developing and developed countries ([6][1]) that defined by the characteristic pattern aggregative adherence (AA) to cultured epithelial cells ([7][2]). Studies from various laboratories have demonstrated
Invasive non-typhoidal Salmonella (iNTS) from the clonal type ST313 (S. Typhimurium ST313) is primary cause of invasive salmonellosis in Africa. Recently, Brazil, iNTS strains have been isolated different sources, but there a lack understanding mechanisms behind how these gut bacteria can break barrier and reach patient's bloodstream. Here, we compare 13 S. ST313, previously unreported isolates, human blood cultures, investigating aspects virulence resistance. Initially, RNAseq analyses...
Glioblastoma (GBM) is the most lethal and frequent type of brain tumor, leading patients to death in approximately 14 months after diagnosis. GBM treatment consists surgical removal followed by radio chemotherapy. However, tumors commonly relapse promotes only a slight increase patient survival. Thus, uncovering cellular mechanisms involved resistance utmost interest, use cell lines has been shown be an extremely important tool. In this work, exploration RNAseq data from different revealed...