A5058776634- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Probiotics and Fermented Foods
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Community Ecology and Physiology
- Parasitic Infections and Diagnostics
- Antibiotics Pharmacokinetics and Efficacy
- Child Nutrition and Water Access
- Antibiotic Resistance in Bacteria
Instituto Nacional de Salud
2012-2023
Instituto Nacional de Salud
2022
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. Following optimization logistics that shipping isolates as thermolysates in ambient conditions, project assembled diverse 10,419 from low- middle-income countries. The genomes were sequenced using LITE library...
ABSTRACT Polymyxins are last-resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae . Due the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed characterize associated with presence mcr-1 nonfermenter Gram-negative bacilli, using isolates collected retrospectively Colombia from 2002 2016. A total 5,887 clinical were studied, 513 found be resistant polymyxins. Susceptibility colistin was confirmed broth...
Shigella sonnei is a globally important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed demonstrate possibility advantages of transitioning whole genome sequencing (WGS) for within existing networks across continent where S. endemic.We applied WGS representative archive isolates (n = 323)...
Little is known about the genetic diversity of Salmonella enterica serovar Typhi ( S . Typhi) circulating in Latin America. It has been observed that typhoid fever still endemic this part world; however, a lack standardized blood culture surveillance across American makes estimating true disease burden problematic. The Colombian National Health Service established system for tracking bacterial pathogens, including Typhi, 2006. Here, we characterized 77 representative isolates collected...
Background.Salmonella enterica subsp. serovar Typhimurium (S. Typhimurium) has been linked to outbreaks of foodborne gastroenteritis disease, and the emergence antimicrobial-resistant clones. In Colombia, laboratory surveillance Salmonella spp. between 1997-2018 revealed that S. was most ubiquitous (27.6 % all isolates), with increasing levels resistance several families antibiotics.Hypothesis. Resistant isolates recovered from human clinical, food swine samples carry class 1 integrons are...
Salmonella Typhi (S. Typhi) is the causative agent of typhoid fever; a systemic disease affecting ~20 million people per year globally. There are little data regarding contemporary epidemiology in Latin America. Consequently, we aimed to describe some recent epidemiological aspects Colombia using cases reported National Public Health Surveillance System (Sivigila) between 2012 and 2015. Over four-year reporting period there were 836 culture confirmed Colombia, with majority (676/836; 80.1%)...
This study aimed to provide evidence of the domestic benefits introducing an integrative genomic analysis from One Health approach in national surveillance Salmonella enterica between 1997-2017 Colombia.Data on clinical laboratory-based and a cross-sectional at chicken retail stores Colombia were compared using phenotypic, molecular, approaches. Additional by serovar single nucleotide polymorphism was developed increase resolution relatedness interfaces.Locally, diversity pathogenic factors...
Introducción. La variante monofásica (1,4,[5],12:i:-) de Salmonella Typhimurium ocupa los primeros lugares en programas vigilancia a nivel mundial. En Colombia, enterica alcanza el cuarto lugar cuanto aislamientos clínicos recuperados por medio la laboratorio del Grupo Microbiología Instituto Nacional Salud, pero se desconoce si dichos están relacionados con que circula global, y sus características genéticas fenotípicas.Objetivo. Caracterizar Colombia entre 2015 2018 Salud.Materiales...
Introduction: Salmonella enterica subsp. serovar Give is found in ruminants, pigs, poultry, and aquatic environments, but rarely humans. In Colombia, this serotype was ranked 11th. the laboratory surveillance of acute diarrheal disease between 2000 2013. Objective: To characterize phenotypic genotypic isolates related to an outbreak foodborne Illness department Vichada fifth epidemiological week 2015. Materials methods: Following Instituto Nacional de Salud method, we tested 37 fecal samples...
Salmonella Enteritidis is recognized worldwide as one of the main agents human gastrointestinal infection. Several reports indicate presence isolates with decreased sensitivity to ciprofloxacin that can lead a delayed response or development resistance during treatment.To describe and characterize associated an outbreak food-borne diseases in Popayán, Cauca.Ten from nine patients food sample (chicken sandwich) were analyzed by biochemical tests, serotyping antimicrobial sensitivity. The...
Shigellosis is endemic in low-and middle-income countries, causing approximately 125 million episodes of diarrhea and leading to 160 .000 deaths annually one-third which associated with children.To describe the characteristics antimicrobial resistance profiles Shigella species recovered Colombia from 1997 2018.We received isolates laboratories 29 Colombian departments. We serotyped specific antiserum determined minimal inhibitory concentrations for ten antibiotics Kirby-Bauer tests following...
Introduction: In Colombia, Shigella sonnei is one of the most frequently isolated serotypes (53.4%) in human clinical samples associated with diarrheal acute disease. The identification DNA restriction patterns by pulsed field gel electrophoresis basis for molecular surveillance S. sonnei.Objective: To establish Colombia using pulsed-field electrophoresis.Materials and methods: We studied 102 2,048 isolates referred National Laboratory Network between 1997 March, 2013; selection was made...
Abstract Foodborne diseases represent a link between environmental, animal, and human health interfaces from the One Health perspective. Whole genome sequencing (WGS) is becoming gold standard in foodborne surveillance, worldwide. WGS than provides precision data pathogens allows laboratories to resolve genetic relations among all sources perspective, especially during outbreak investigations, getting insights into their transmission routes pathogenicity. Salmonella spp. most prevalent...
Introducción. Salmonella spp. es un agente patógeno zoonótico transmitido al humano por el agua o los alimentos contaminados. La presencia de β-lactamasas espectro extendido creciente problema para la salud pública debido a que estas enzimas confieren resistencia contra las cefalosporinas tercera y cuarta generación.Objetivo. Caracterizar en aislamientos recibidos programa vigilancia enfermedad diarreica aguda transmitida del Grupo Microbiología Instituto Nacional Salud. Materiales métodos....
Introduction. Salmonella Enteritidis is recognized worldwide as one of the main agents human gastrointestinal infection. Several reports indicate presence isolates with decreased sensitivity to ciprofloxacin that can lead a delayed response or development resistance during treatment. Objective. To describe and characterize associated an outbreak food-borne diseases in Popayán, Cauca. Materials methods. Ten from nine patients food sample (chicken sandwich) were analyzed by biochemical tests,...
Abstract Background Salmonella enterica subsp. serovar Typhimurium ( S. Typhimurium) has been linked to outbreaks of foodborne gastroenteritis disease, and the emergence antimicrobial-resistant clones. In Colombia, laboratory surveillance spp. between 1997-2018 revealed that S . was most ubiquitous (27.57% all isolates), with increasing levels antimicrobial resistance several families antibiotics. Hypothesis Resistant isolates recovered from clinical swine samples carry class 1 integrons are...
Aim: Different species of the genus Vibrio are pathogenic for humans, aim this study was to standardize a multiplex PCR identify four species. Methods. 126 isolates environmental and 66 from surveillance program INS were analyzed by species-specific genes. Results. Of total isolates, 124 positive one The achieved identification target (V. parahaemolyticus, V. alginolyticus, vulnificus fluvialis), showing specificity sensitivity between 1-2 ng/ul. Discussion. A developed that could provide reliable