A5050817345- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Plant Pathogenic Bacteria Studies
- Mosquito-borne diseases and control
- Pharmaceutical Practices and Patient Outcomes
- Urinary Tract Infections Management
- Healthcare Systems and Reforms
- Probiotics and Fermented Foods
- Nosocomial Infections in ICU
- Dengue and Mosquito Control Research
- COVID-19 Digital Contact Tracing
- Antifungal resistance and susceptibility
- Kidney Stones and Urolithiasis Treatments
- Microbial Community Ecology and Physiology
- Sepsis Diagnosis and Treatment
- Vaccine Coverage and Hesitancy
Universidad El Bosque
2017-2025
Hospital San Borja Arriarán
2024
Centro Internacional de Entrenamiento e Investigaciones Medicas
2011-2022
Carbapenem resistance is a critical healthcare challenge worldwide. Particularly concerning the widespread dissemination of Klebsiella pneumoniae carbapenemase (KPC). harboring blaKPC (KPC-Kpn) endemic in many areas including United States, where epidemic was primarily mediated by clonal Kpn ST258. We postulated that spread other regions occurs different and more complex mechanisms. To test this, we investigated evolution dynamics KPC-Kpn Colombia, KPC became rapidly after emerging 2005.We...
ABSTRACT Polymyxins are last-resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae . Due the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed characterize associated with presence mcr-1 nonfermenter Gram-negative bacilli, using isolates collected retrospectively Colombia from 2002 2016. A total 5,887 clinical were studied, 513 found be resistant polymyxins. Susceptibility colistin was confirmed broth...
Carbapenem-resistant Enterobacterales (CRE) pose a significant threat to global public health. The most important mechanism for carbapenem resistance is the production of carbapenemases. Klebsiella pneumoniae carbapenemase (KPC) represents one main carbapenemases worldwide. Complex mechanisms bla KPC dissemination have been reported in Colombia, country with high endemicity resistance. Here, we characterized dynamics gene among CRE infecting and colonizing patients three hospitals localized...
Abstract Background Antimicrobial stewardship programs (ASPs) have become a fundamental pillar in optimizing antimicrobial usage, improving patient care, and reducing resistance (AMR). Herein we evaluated the impact of an ASP on consumption AMR Colombia. Methods We designed retrospective observational study measured trends antibiotic before after implementation using interrupted time series analysis over 4-year period (24 months 24 implementation). Results ASPs were implemented according to...
Ceftazidime-avibactam (CZA) is the combination of a third-generation cephalosporin and new non-β-lactam β-lactamase inhibitor capable inactivating class A, C, some D β-lactamases. From collection 2,727 clinical isolates Enterobacterales (n = 2,235) P. aeruginosa 492) that were collected between 2016 2017 from five Latin American countries, we investigated molecular resistance mechanisms to CZA 127 (18/2,235 [0.8%] 109/492 [22.1%] aeruginosa). First, by qPCR for presence genes encoding KPC,...
Introduction: The continuous evolution of antimicrobial resistance poses a major threat to public health worldwide. Molecular biology techniques have been integrated epidemiological surveillance systems improve the control strategies this phenomenon.Objective: To describe phenotypic and molecular profiles most important Gram negative bacilli from intensive care units in 23 Colombian hospitals during study period 2009-2012.Materials methods: A descriptive was conducted belonging Nosocomial...
This study aimed to evaluate the susceptibility of clinical isolates Enterobacterales and Pseudomonas aeruginosa fosfomycin determine concordance disk diffusion (DD) broth microdilution (BMD) with agar dilution (AD) for testing.The activity against 225 Escherichia coli (n = 64), Klebsiella pneumoniae 68), Enterobacter spp. 28) P. 65) was tested by AD, DD BMD. For DD, results were recorded considering not colonies growing within inhibition halo as recommended CLSI EUCAST, respectively....
Abstract Background Colombia has been endemic for KPC decades, mainly due to the clonal dissemination of blaKPC-3 among carbapenemase-producing K. pneumoniae (CP-Kp) sequence type (ST) 258 and plasmid blaKPC-2 different species Gram-negatives. However, a recent surge in detection blaNDM CP-Kp raised concerns. Herein, we aimed understand dynamics transmission carbapenemase genes clinical isolates two cities Colombia.Fig. 1.Molecular characterization 31 collected between 2018 - 2019.MLST:...
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen with increase in the frequency of infections caused by multidrug resistant (MDR) and extensively drug (XDR) strains, limiting available therapeutic options. The most troublesome resistance acquisition production carbapenemases such as Verona integron-encoded metallo-β-lactamases (VIM), frequent widespread, Klebsiella pneumoniae (KPC), which has continuously spread last decade. Its dissemination linked to their location on...
We report complete genome sequences of four blaNDM-1-harboring Gram-negative multidrug-resistant (MDR) isolates from Colombia. The blaNDM-1 genes were located on 193-kb Inc FIA, 178-kb A/C2, and 47-kb (unknown type) plasmids. Multilocus sequence typing (MLST) revealed that these belong to type 10 (ST10) (Escherichia coli), ST392 (Klebsiella pneumoniae), ST322 ST464 (Acinetobacter baumannii Acinetobacter nosocomialis, respectively). Our analysis identified the A/C2 plasmid in E. coli...
ABSTRACT We report the emergence of a novel VIM variant (VIM-24) in Klebsiella pneumoniae isolate Colombia. The displays MICs for carbapenems below resistance breakpoints, posing real challenge its detection. bla VIM-24 gene was located within class 1 integron carried on large plasmid. Further studies are needed to clarify epidemiological and clinical impact.
La infección no complicada del tracto urinario bajo y la bacteriuria asintomática son causas frecuentes de consulta médica ambulatoria en el servicio urgencias Colombia mundo. falta pautas consenso para manejo, así como emergencia resistencia a las múltiples opciones terapéuticas disponibles los uropatógenos provenientes comunidad, hacen necesario elaborar unas recomendaciones que orienten al clínico sobre abordaje óptimo estas entidades. Definir un manejo empírico (ITU) adultos mujeres...
Two pairs of ceftolozane/tazobactam susceptible/resistant P. aeruginosa were isolated from 2 patients after exposure to β -lactams. The genetic basis resistance was evaluated, and -lactam-resistant mechanisms assessed by phenotypic assays. Whole genome sequencing identified mutations in AmpC including the mutation (V213A) a deletion 7 amino acids (P210–G216) Ω-loop. Phenotypic assays showed that strain with V213A variant associated increased -lactamase hydrolysis activity. On other hand,...
Studies have shown that more than 50% of the antibiotics used in hospitals are unnecessary or inappropriate and, antimicrobial resistance may cost up to 20 billion USD excess medical costs each year. On other hand, Antimicrobial Stewardship Programs (ASP) significantly reduce use, emergence resistance, healthcare associated infections, and hospital settings.To evaluate development ASP antibiotic savings 7 Latin American using standardized quantitative indicators all participating health care...
We report the presence of mcr-1 gene among 880 Escherichia coli clinical isolates collected in 13 hospitals from 12 Colombian cities between 2016 and 2019. Seven (0.8%) were colistin resistant (MIC ≥ 4 µg/mL). These colistin-resistant screened for gene; five carried gene. subjected to whole genome sequencing (WGS) identify additional resistomes their ST. In addition, antimicrobial susceptibility testing revealed that all E. carrying susceptible third generation-cephalosporin carbapenems,...
Resistance to ceftazidime-avibactam (CZA) due
Background: High rates of resistance to third-generation cephalosporins and carbapenems in Enterobacterales have been reported Latin America. Ceftazidime/avibactam (CZA) is the combination a cephalosporin non-β-lactam β-lactamase inhibitor, which has shown activity against isolates producing class A, C D β-lactamases. Herein, we evaluated CZA comparators clinical Methods: The was from Argentina, Brazil, Chile, Colombia Mexico that were collected between January 2016 October 2017. One...
Background: The dissemination of the uropathogenic O25b-ST131 Escherichia coli clone constitutes a threat to public health. We aimed determine circulation E. strains belonging O25b:H4-B2-ST131 and H30-Rx epidemic subclone causing hospital community-acquired urinary tract infections (UTI) in Colombia. Methods: Twenty-six nonduplicate, CTX-M group-1-producing isolates UTI community were selected for this study. Results: Twenty-two harboring CTX-M-15, one CTX-M-3, three CTX-M-55 identified....
Polymyxin resistance in Klebsiella pneumoniae has been attributed to mutations mgrB, phoPQ, pmrAB, and crrAB the presence of mcr plasmid-mediated genes. Herein, we describe molecular characteristics 24 polymyxin- carbapenem-resistant K. isolates recovered from six Colombian cities between 2009 2019. Minimum inhibitory concentrations (MICs) polymyxin were confirmed by broth microdilution, whole-genome sequencing was performed determine sequence type, resistome, genes related resistance, as...
Identify molecular mechanisms responsible for the in vitro non-susceptibility to ceftolozane/tazobactam (TOL) a group of 158 clinical isolates Pseudomonas aeruginosa from five Latin American countries collected before introduction TOL into practice.Clinical P. (n = 504) were between January 2016 and October 2017 20 hospitals located Argentina, Brazil, Chile, Colombia, Mexico. Minimum inhibitory concentrations (MICs) determined by standard broth microdilution interpreted according CLSI...
Introducción. El comportamiento de la resistencia antimicrobiana es fundamental en el mejoramiento y ajuste los programas optimización uso antimicrobianos, implementación las guías terapéuticas precauciones que limitan transmisión cruzada bacterias resistentes entre pacientes. Desde inicio del 2020, pandemia SARS-CoV-2 desafió profundamente al sistema salud y, según algunos reportes, aumentó tasas antimicrobiana.Objetivo. Describir microrganismos más frecuentes veinte hospitales colombianos...