A5080167570- Environmental Toxicology and Ecotoxicology
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Pharmaceutical and Antibiotic Environmental Impacts
- Helicobacter pylori-related gastroenterology studies
- RNA Research and Splicing
- Cancer Research and Treatments
- Reproductive biology and impacts on aquatic species
- Hedgehog Signaling Pathway Studies
- Genomics and Chromatin Dynamics
- Body Contouring and Surgery
- Wnt/β-catenin signaling in development and cancer
- Pancreatic and Hepatic Oncology Research
- Metastasis and carcinoma case studies
- Geography and Environmental Studies
- Heavy metals in environment
- Genetic factors in colorectal cancer
- Marine Ecology and Invasive Species
Universidade do Porto
2012-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2021-2024
IPO Porto
2022-2023
Centro Interdisciplinar de Investigação Marinha e Ambiental
2015
Institute of Biomedical Science
2014
University of Aveiro
2009
Recent findings indicate that different Endocrine Disrupting Chemicals (EDCs) interfere with lipid metabolic pathways in mammals and promote fat accumulation, a previously unknown site of action for these compounds. The antifoulant environmental pollutant tributyltin (TBT), which causes imposex gastropod snails, induces an "obesogenic" phenotype mammals, through the activation nuclear receptors retinoid X receptor (RXR) peroxisome proliferator-activated gamma (PPARγ). In teleosts, effects...
Abstract Background Germline CDH1 pathogenic or likely variants cause hereditary diffuse gastric cancer (HDGC). Once a genetic is identified, stomachs’ and breasts’ surveillance and/or prophylactic surgery offered to asymptomatic carriers, which life-saving. Herein, we characterized an inherited mechanism responsible for extremely early-onset atypical HDGC high penetrance. Methods Whole-exome sequencing (WES) re-analysis was performed in unsolved family. Accessible chromatin promoter...
Both field and experimental data examined the influence of exposure to environmental contaminant tributyltin (TBT) on marine organisms. Although most attention focused imposex phenomenon in gastropods, adverse effects were also observed other taxonomic groups. It has been shown that induction involves modulation retinoid signaling gastropods. Whether TBT influences similar pathways fish is yet be addressed. In this study, larvae model teleost Danio rerio exposed natural retinoids,...
The effects of endotoxin on endothelial and smooth muscle function were investigated in small femoral arteries removed from rats 4 h after intraperitoneal injection Escherichia coli lipopolysaccharide (LPS; 20 mg/kg) or solvent. In the absence L-arginine organ bath, sensitivity to norepinephrine (NE) was decreased only slightly, relaxing neither 3-morpholinosydonimine-N-ethyl-carbamide (SIN-1), a nitric oxide (NO) donor, nor acetylcholine (ACh) modified by LPS treatment despite morphological...
E-cadherin is a key player in gastric cancer (GC) and germline alterations of CDH1, its encoding gene, are responsible for Hereditary Diffuse Gastric Cancer (HDGC) syndrome. This study aimed at elucidating the role genetic variants DNA methylation CDH1 promoter enhancers regulation gene expression. For this purpose, we analyzed through Next-Generation Sequencing (NGS) series GC cell lines (NCI-N87, KATO-III, SNU-1, SNU-5, GK2, AKG, KKP) corresponding expression levels. By bisulfite genomic...
Surgical resection with lymphadenectomy and perioperative chemotherapy is the universal mainstay for curative treatment of gastric cancer (GC) patients locoregional disease. However, GC survival remains asymmetric in West- East-world regions. We hypothesize that this asymmetry derives from differential clinical management. Therefore, we collected chemo-naïve Portugal South Korea to explore specific immunophenotypic profiles related disease aggressiveness clinicopathological factors...
Hereditary diffuse gastric cancer (HDGC) caused by CDH1 variants predisposes to early-onset (DGC) and lobular breast (LBC). In Northern Portugal, the unusually high number of HDGC cases in unrelated families carrying c.1901C>T variant (formerly known as p.A634V) suggested this a CDH1-founder variant. We aimed demonstrate that is bona fide truncating inducing cryptic splicing, calculate timing potential founder effect, characterize tumour spectrum age onset families. The impact splicing...
Cadherins are cell-cell adhesion molecules, fundamental for cell architecture and polarity. E-cadherin to P-cadherin switch can rescue adherens junctions in epithelial tumours. Herein, we disclose a mechanism gastric cancers. CDH1 CDH3 mRNA expression was obtained from 42 tumours' RNA-seq data. CRISPR-Cas9 used knock out putative regulatory element. CDH1-depleted parental cells were submitted proteomics enrichment GO terms analysis; ATAC-seq/4C-seq with promoter viewpoint assess chromatin...
In gastric cancer (GC), biomarkers that define prognosis and predict treatment response remain scarce. We hypothesized the extent of CD44v6 membranous tumor expression could therapy in GC patients. Two surgical cohorts, from Portugal South Korea (n = 964), were characterized for extension immuno-expression, clinicopathological features, patient survival, response. The value predicting to its impact on was determined. High associated with invasive features (perineural invasion depth invasion)...
Interference of organotin with animal endocrine systems represents a notable case physiological disruption. Caenogastropod molluscs are particularly sensitive to exposure these compounds, developing condition termed imposex, the superimposition male sexual secondary features onto females. Recently, various studies have shown that retinoic X receptor is high-affinity ligand tributyltin (TBT), while simultaneously hampering expression gene. Curiously, in ascidians TBT has been downregulate...
Transitions between epithelial and mesenchymal cellular states (EMT/MET) contribute to cancer progression. We hypothesize that EMT followed by MET promotes cell population heterogeneity, favouring tumour growth. developed an model on off exposure of EpH4 cells (E-cells) TGFβ1 mimics phenotypic (M-cells) MET. aimed at understanding whether is accompanied molecular functional reversion back epithelia using RNA sequencing, immunofluorescence (IF), proliferation, wound healing, focus formation...
Abstract Missing heritability in hereditary diffuse gastric cancer (HDGC) ranges from 60 to 90%. These HDGC-like families, despite complying with HDGC clinical criteria, lack CDH1 and CTNNA1 actionable germline variants, are not offered HDGC-targeted life-saving disease prevention measures. Herein, we explored novel predisposition mechanisms affecting the -regulatory network. We called single-nucleotide (SNV) copy-number variants (CNV) 19 probands whole-genome sequencing data performed...
Abstract: This paper aims to analyze the horizontal displacements of Capanda DAM by using 3D DIANA Finite Element Analysis, which suits its complex shell structure. The model was also used behavior considering both hydrostatic pressure and geotechnical characterization, afterwards, it noted that loads changed code more restricted in design formulae. In addition, are defined database according software closed codes. this research, is fully described gravity type reinforced concrete, with a...
Abstract: The research presents a detailed in-service lateral buckling analysis on the water injection (WI9) line of Angola Block 15/06 Agogo 2 Oil field located Development Project. was performed following methodology given by Flowline Global Buckling Design Premises. study aims at assessment susceptibility to buckling, calculation through FE (if necessary), bending levels reached in post-buckling configuration, design corrective measures ensuring safety pipelines and fulfillment pipe...
Abstract Simvastatin (SIM), a hypocholesterolaemic compound, is among the most prescribed pharmaceuticals for cardiovascular disease prevention worldwide. Several studies have shown that acute exposure to SIM able produce multiple adverse effects in aquatic organisms. However, uncertainties still remain regarding chronic of ecosystems. Therefore, present study aimed investigate model freshwater teleost zebrafish ( Danio rerio ) following (90 days) environmentally relevant concentrations...