A5090963887- Drug Transport and Resistance Mechanisms
- Cancer therapeutics and mechanisms
- Pharmacological Effects and Toxicity Studies
- Trace Elements in Health
- HIV/AIDS drug development and treatment
- Nanoparticle-Based Drug Delivery
- Amino Acid Enzymes and Metabolism
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
- DNA and Nucleic Acid Chemistry
- Metabolism, Diabetes, and Cancer
- Systemic Lupus Erythematosus Research
- Malaria Research and Control
- Monoclonal and Polyclonal Antibodies Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Radiomics and Machine Learning in Medical Imaging
- AI in cancer detection
- Chronic Lymphocytic Leukemia Research
- ATP Synthase and ATPases Research
- Nanocluster Synthesis and Applications
- Rheumatoid Arthritis Research and Therapies
- Lipid Membrane Structure and Behavior
Wrightington, Wigan and Leigh NHS Foundation Trust
2024
South Dakota State University
2015-2022
American University of Iraq Sulaimani
2022
Loyola University Chicago
2014-2015
University of Minnesota
2015
Queen's University
2010-2013
Columbia University
2007-2008
University of Illinois Urbana-Champaign
2005
ABSTRACT Based on its genome sequence, the pathway of β-oxidative fatty acid degradation in Salmonella enterica serovar Typhimurium LT2 has been thought to be identical well-characterized Escherichia coli K-12 system. We report that wild-type strains S. grow decanoic acid, whereas E. cannot. Mutant (carrying fadR ) both organisms which genes ( fad are expressed constitutively readily isolated. The more rapidly than and also well octanoic hexanoic acids (which do not support growth strains)....
Multidrug resistance protein 1 (MRP1/ABCC1), an integral transmembrane efflux transporter, belongs to the ATP-binding cassette (ABC) superfamily. MRP1 governs absorption and disposition of a wide variety endogenous xenobiotic substrates including various drugs across organs physiological barriers. Additionally, its overexpression has been implicated in multidrug chemotherapy multiple cancers. Here, we describe development high content imaging-based screening assay for activity. This live...
Multidrug resistance protein 1 (MRP1/ABCC1) actively transports a variety of drugs, toxic molecules and important physiological substrates across the plasma membrane. It can confer broad-spectrum multidrug decrease bioavailability many drugs. Substrates MRP1 include anti-cancer agents, antibiotics, antivirals, antidepressants anti-inflammatory Using calcein as fluorescent reporter in high content uptake assay, we recently reported identification 12 inhibitors after screening an library 386...
The SNF1/AMP-activated protein kinase (AMPK) family is required for adaptation to metabolic stress and energy homeostasis. gamma subunit of AMPK binds AMP ATP, mutations that affect binding cause human disease. We have here addressed the role Snf4 (gamma) in regulating SNF1 response glucose availability Saccharomyces cerevisiae. Previous studies mutant cells lacking suggested counteracts autoinhibition by C-terminal sequence Snf1 catalytic but dispensable regulation, does not activate vitro....
The FadR protein of Escherichia coli has been shown to play a dual role in transcription the genes bacterial fatty acid metabolism. acts as repressor beta-oxidation and an activator unsaturated synthesis. DNA binding is antagonized by long chain acyl-CoAs, thus sensor availability environment. When viewed from genomic viewpoint, proteins are unusual that domain very highly conserved among FadR-containing bacteria, whereas C-terminal acyl-CoA shows only weak conservation. To further our...
Efflux transporters P-glycoprotein (P-gp/ABCB1), multidrug resistance protein 1 (MRP1/ABCC1), and breast cancer (BCRP/ABCG2) can affect the efficacy toxicity of a wide variety drugs are implicated in (MDR). Eight test compounds, recently identified from an intramolecular FRET-based high throughput screening, were characterized for their interaction with MRP1. We report that active metabolite vitamin D<sub>3</sub>, calcitriol, its analog calcipotriol selectively cytotoxic to...
Multidrug resistance protein 1 (MRP1) actively transports a wide variety of drugs out cells. To quantify MRP1 structural dynamics, we engineered "two-color MRP1" construct by fusing green fluorescent (GFP) and TagRFP to nucleotide–binding domains NBD1 NBD2, respectively. The recombinant expressed trafficked normally the plasma membrane. Two-color transport activity was normal, as shown vesicular [<sup>3</sup>H]17<i>β</i>-estradiol-17-<i>β</i>-(d-glucuronide) doxorubicin efflux in AAV-293 We...
Cancer is a global epidemic disease responsible for over ten millions death worldwide. The early diagnosis and the precise treatment with reduced adverse reactions are main goal In this study, we produced, characterized evaluated (in vitro) in three different cancer cell lines (protaste, breast melanoma) radioactive gold nanocluster (R-AuNC) (198Au25(Capt)18). pharmacokinetics as influence ABC transporter (MRP1 Efflux Transporter Protein) was also evaluated. results showed that R-AuNC...
The cancer multidrug resistance is involved in the failure of several treatments during treatment. It a phenomenon that has been receiving great attention last years due to sheer amount mechanisms discovered and process which hinders effectiveness many anti-cancer drugs. Among resistance, participation ATP-binding cassette (ABC) transporters main one. ABC are group plasma membrane intracellular organelle proteins externalization substrates from cells, expressed cancer. They clearance...
Multidrug resistance protein 1 (MRP1) can efflux a wide variety of molecules including toxic chemicals, drugs, and their derivatives out cells. Substrates MRP1 include anti-cancer agents, antibiotics, anti-virals, anti-human immunodeficiency virus (HIV), many other drugs. To identify novel substrates modulators by exploiting intramolecular fluorescence resonance energy transfer (FRET), we genetically engineered six different two-color proteins changing green fluorescent (GFP) insertion...
Hepatocellular carcinoma (HCC) is the third-leading cause of cancer death in world, with outlook for most patients having a 5-year survivability less than 5%. In previous study from our laboratory, novel estrone inspired analogs act as epidermal growth factor receptor (EGFR) inhibitors HepG2 cells. This focuses on effect these an HCC cell line resistance to Erlotinib. Lead compounds MMA132 and MMA102 showed 13 20 µM IC50 values, respectively against HepG2-R resistant These cycle arrest G2...