Hepatic macrophages account for the largest non-parenchymal cell population in liver. Recent studies have found that hepatic different functions stages of experimental liver fibrosis. Some there are types liver, although others suggested could switch to phenotypes environments. Many demonstrated while promoted fibrosis through recruitment proinflammatory immune cells, and secretion cytokines chemokines early stages, these also resolution matrix metalloproteinases late stages. This article...

ABSTRACT The presence or absence of cytogenetic mutations is proposed to be responsible for the pathogenesis acute myeloid leukaemia (AML). However, current classification system inadequate elucidate molecular heterogeneity disease, and therapy failures frequently occur. Leukaemia stem cells (LSCs) initiate maintain clonal hierarchy AML exhibit properties self-renewal remaining recalcitrant conventional chemotherapy. In this study, we identified a novel long non-coding RNA (lncRNA) MAGI2...

Human serum amyloid P (hSAP), a member of the pentraxin family, inhibits activation fibrocytes in culture and experimental renal, lung, skin cardiac fibrosis. As hepatic inflammation is one causes liver fibrosis, present study, we investigated hepatoprotective effects hSAP against carbon tetrachloride (CCl4)-induced injury. Our data indicated that attenuated histopathological abnormalities significantly decreased inflammatory cell infiltration pro-inflammatory factor expression. Moreover,...

Abstract Understanding the changes of activated HSCs reversion is an essential step toward clarifying potential roles in treatment liver fibrosis. In this study, we chose adipocyte differentiation mixture to induce LX-2 cells for 2 days vitro as phase, comparing with normal cultured activation phase. Mass spectrometric-based SILAC technology was adopted study differentially expressed proteome between and activation. Compared HSCs, 273 proteins showed significant differences reverted HSCs....

Cancer cells-secreted extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in local and distant microenvironment. Our initial GEO database analysis identified the presence differentially-expressed microRNA-1246 (miR-1246) acute myeloid leukemia (AML) cell-derived EVs. Consequently, current study set out to investigate role AML-derived EVs-packaged miR-1246 stem cells (LSCs) bioactivities. The predicted binding between LRIG1 was verified using dual...

The role of LOXL1 in fibrosis via mediating ECM crosslinking and stabilization is well established; however, the hepatic stellate cells (HSCs)-specific development remains unknown. We generated HSCs-specific Loxl1-depleted mice (Loxl1Gfap-cre mice) to investigate contribution pathogenesis fibrosis. Loxl1fl/fl were used as control. Furthermore, we RNA sequencing explore underlying changes transcriptome. Results sirius red staining, type I collagen immunolabeling, hydroxyproline content...

Endometriosis is an estrogen-dependent gynecological disease primarily affecting women of childbearing age, which gives rise to pelvic pain calling for multiple operations, and sometimes leading infertility. However, the etiology endometriosis remains poorly understood. In this study we investigated roles two Ubiquitin E3 Ligases, namely hsc70-interacting protein (CHIP) mouse double minute 2 (MDM2), in abnormal estrogenic activity endometriosis. We first collected endometrial tissues from 91...

Abstract The aim of this study was to optimise a collagenase perfusion protocol for the isolation liver non‐parenchymal cell (NPC) suspension enriched Kupffer cells that reduced damage F4/80 antigen surface expression allow analysis by flow cytometry. cell‐enriched NPCs were isolated from C57BL/6 mice using different protocols. Flow cytometry used examine effect digestion on cells, and results represented percentage positive mean fluorescence intensity (MFI). temperature, concentration...

We reported previously that B16, JB/RH and JB/MS melanomas of C57BL/6 mice express the common melanoma-associated antigen (MAA) recognized by MM2-9B6 monoclonal antibody (MAb). This MAA is encoded env gene an ecotropic MuLV-type retrovirus somatically emerged in mice. The potential role this (MelARV) melanoma formation remains unknown has not been investigated. To test this, normal melanocyte lines (melan-a C57M) were infected with MelARV produced B16BL6 melanoma. Infection these melanocytes...

Abstract Background Kupffer cells (KCs) originate from yolk-sac progenitors before birth. Throughout adulthood, they self-maintain independently the input of circulating monocytes (MOs) at a steady state and are replenished within 2 weeks after having been depleted, but origin repopulating KCs in adults remains unclear. The current paradigm dictates that preexisting or monocytes, there lack fate-mapping evidence. Methods We first traced fate monocytic with tissue-resident macrophage-specific...

Abstract Background & Aims: Lysyl oxidase-like-1 (LOXL1), a vital crosslinking enzyme in extracellular matrix (ECM) protein maintenance, is well established fibrosis via mediating ECM stabilization. However, the potential role of LOXL1 pathogenesis nonalcoholic steatohepatitis (NASH) has not been previously studied. Methods: We generated Loxl1 fl/fl mice to selectively delete hepatic stellate cells (HSCs) (Loxl1 Gfap cre ; as littermate controls) and then examined liver pathology...

Advanced Persistent Threat (APT) is challenging to detect due prolonged duration, infrequent occurrence, and adept concealment techniques. Existing approaches primarily concentrate on the observable traits of attack behaviors, neglecting intricate relationships formed throughout persistent lifecycle. Thus, we present an innovative APT detection framework named LTRDetector, implementing end-to-end holistic operation. LTRDetector employs graph embedding technique retain comprehensive...

Myeloid cells infiltrate into the liver and differentiate macrophages in different injury mouse models. However, heterogeneity of bone marrow (BM)-derived LMs populations remains to be understood. To investigate this understand impact macrophage niche on properties recruited BM-derived macrophages, we used a non-myeloablation BM transplantation model label trace LMs. Subsequently, quantified number embryonic-derived liver-resident total CCl4 irradiated acute models, respectively. Finally,...

We reported previously that B16, JB/RH and JB/MS melanomas of C57BL/6 mice express the common melanoma-associated antigen (MAA) recognized by MM2-9B6 monoclonal antibody (MAb). This MAA is encoded env gene an ecotropic MuLV-type retrovirus somatically emerged in mice. The potential role this (MelARV) melanoma formation remains unknown has not been investigated. To test this, normal melanocyte lines (melan-a C57M) were infected with MelARV produced B16BL6 melanoma. Infection these melanocytes...

The characterization of DNA synthesis and cell proliferation in vitro by 5-ethynyl-2′-deoxyuridine (EdU) incorporation is widely used, but this has not been optimized for vivo use. In study, the administration route, dose (0–50 mg kg−1), duration (0–72 h) EdU procedure preparation flow cytometry were assay bone marrow cells. percentage EdU-positive cells was higher following intravenous than intraperitoneal injection (p < 0.05); positive increased a dose-dependent manner from 0 to 50 kg−1...

Abstract Kupffer cells (KCs) originate from yolk sac progenitors before birth. Throughout adulthood, they self-maintain independently the input of circulating monocytes (MOs) at stead state, and are replenished within 2 weeks after having been depleted, but origin repopulating KCs in adult remains unclear. The current paradigm dictates that preexisting or monocytes, there a lack fate-mapping evidence. In study, we firstly traced fate monocytic with tissue-resident macrophage-specific...

Abstract Kupffer cells (KCs) originate from yolk sac progenitors before birth, but the origin of repopulating KCs in adult remains unclear. In current study, we firstly traced fate preexisting and that monocytic with tissue-resident macrophage-specific cell-specific mapping mouse models, respectively, found no evidences or MOs. Secondly, performed genetic lineage tracing to determine type progenitor involved response KC depletion mice, depletion, hematopoietic stem (HSCs) proliferated bone...

Background & Aims: Lysyl oxidase-like-1 (LOXL1), a vital crosslinking enzyme in extracellular matrix (ECM) protein maintenance, is well established fibrosis via mediating ECM stabilization. However, the potential role of LOXL1 pathogenesis nonalcoholic steatohepatitis (NASH) has not been previously studied. Methods: We generated Loxl1 fl/fl mice to selectively delete hepatic stellate cells (HSCs) (Loxl1fl/flGfapcre; Loxl1fl/fl as littermate controls) and then examined liver pathology...