A5075872638- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Innovations in Medical Education
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Child and Adolescent Health
- Obesity, Physical Activity, Diet
- Maternal Mental Health During Pregnancy and Postpartum
- Animal Virus Infections Studies
- Insect symbiosis and bacterial influences
- Virus-based gene therapy research
- Interpreting and Communication in Healthcare
- Obesity and Health Practices
- Cultural Competency in Health Care
- Emergency and Acute Care Studies
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Childhood Cancer Survivors' Quality of Life
- Empathy and Medical Education
- Eating Disorders and Behaviors
- Long-Term Effects of COVID-19
- Viral Infections and Immunology Research
- Invertebrate Immune Response Mechanisms
Johns Hopkins Medicine
2015-2025
Johns Hopkins University
2015-2025
Johns Hopkins All Children's Hospital
2015-2025
Center for Health and Gender Equity
2025
Children's Center
2023
Children’s Institute
2021
North Carolina State University
2009-2020
Indiana University
2016
University of Colorado Denver
2013-2014
Children's Mercy Hospital
2014
During the COVID-19 pandemic, antivaccination social media accounts are proliferating online, threatening to further escalate vaccine hesitancy related vaccine. This commentary seeks alert and encourage health care provider community, including professionals academic organizations, engage in counter mounting vaccine-related infodemic. To validate our recommendation for engagement, authors describe preliminary findings using a mixed methods approach of quantitative Twitter-based ranking...
Objective: To compare parental report of child body image with perceived healthy weight in preschoolers and describe weight-counseling preferences. Methods: Parents seeking well-child care were interviewed asked to select images resembling: ( a) their own child’s current weight, b) a preschooler, c) friend family preschooler. Those indicating that overweight or obese resembled considered misclassify weight. Logistic regression was used identify predictors misclassification card-sorting...
Objective: This study outlines the construction and initial psychometric properties of Coronavirus Impact Scale in multiple large diverse samples families with children adolescents. The scale was established to capture impact coronavirus pandemic. Differences between internal structure within were assessed.Method: Five hundred, seventy-two caregivers adolescents or expecting mothers clinical research settings completed Scale. Samples differed developmental stage, background,...
Primary cultures of chick embryo fibroblasts (CEF) are widely used for the cultivation viruses. Protocols growth and maintenance CEF cells in laboratory provided.
It is widely held that arboviruses such as the alphavirus Sindbis virus gain entry into cells by a process of receptor-mediated endocytosis followed membrane fusion in acid environment endosome. We have used an approach direct observation electron microscopy and immunolabeling proteins with antibodies conjugated to gold beads. found upon attaching cell surface, intact RNA-containing viruses became empty shells could be identified only antibody labeling. rate at which full particles were...
Insect bites and the associated hypersensitivity reactions known as papular urticaria account for a significant number of all referrals from pediatricians dermatologists to our pediatric dermatology clinic. Unfortunately, children affected by these eruptions are frequently misdiagnosed often subject expensive evaluations including invasive unnecessary procedures. Here we review course 4 with typical physical findings natural history reactions. On basis clinical experience this patient...
ABSTRACT Cholesterol has been shown to be essential for the fusion of alphaviruses with artificial membranes (liposomes). also implicated as playing an and critical role in processes entry egress living cells. Paradoxically, insects, alternate host alphaviruses, are cholesterol auxotrophs contain very low levels this sterol. To further evaluate life cycle alphavirus Sindbis produced from three different mosquito ( Aedes albopictus ) cell lines; one other insect line, Sf21 Spodoptera...
The prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, virus shuttles from an insect vector to vertebrate and back in complex transmission cycle. must, therefore, be able replicate two biochemically genetically divergent hosts, invertebrates vertebrates. laboratory, grows high titers large variety cultured cells both invertebrate origin. is easily titered for infectivity on several mammalian cell lines as well certain mosquito cells. Full-length cDNA clones...
Bafilomycin A(1) is a specific inhibitor of the vacuolar-ATPase (V-ATPase), which responsible for pH homeostasis cell and acidification endosomes. has been commonly used as method inhibition infection by viruses known or suspected to follow path receptor-mediated endocytosis low-pH-mediated membrane fusion. The exact entry Sindbis virus, prototype alphavirus, remains undetermined. To further investigate role V-ATPase in virus infection, effects bafilomycin on BHK insect cells were studied....
To evaluate whether educational sessions on interpreter use and experience with interpreters are associated resident self-efficacy in the of professional interpreters.In 2010, authors surveyed residents from seven pediatric residency programs. Their 29-item survey collected data training (1) determining when an is needed (2) using a interpreter. The conducted bivariate multivariate regression analyses.Among 271 respondents, 82% reported that ≥ 10% their patients had limited English...
Theoretical framework: Information on the prevalence of post-COVID-19 symptoms and their impact functional status is variable. Objective: To characterize persistent symptoms, depressive patients. Method: Observational, cross-sectional, descriptive study. Instruments were applied via telephone (4-25 weeks post-discharge): Cross-cultural adaptation C19-YRS Questionnaire, Post COVID-19 Functional Status Scale, CES-D. Results: Hypertension was most frequent comorbidity; persistence pain (66.6%)...
Theoretical framework: Information on the prevalence of post-COVID-19 symptoms and their impact functional status is variable. Objective: To characterize persistent symptoms, depressive patients. Method: Observational, cross-sectional, descriptive study. Instruments were applied via telephone (4-25 weeks post-discharge): Cross-cultural adaptation C19-YRS Questionnaire, Post COVID-19 Functional Status Scale, CES-D. Results: Hypertension was most frequent comorbidity; persistence pain (66.6%)...
Abstract The current study examines the application of Pediatric-Buccal-Epigenetic (PedBE) clock, designed for buccal epithelial cells, to endothelia. We evaluate association PedBE epigenetic age and acceleration estimated from human umbilical vein endothelial cells (HUVECs) with length gestation birthweight in a racially ethnically diverse sample (analytic n = 333). was positively associated gestational at birth ( r 0.22, p < .001) infant weight 0.20, .001). Multivariate models revealed...
Aims: The aim of this study is to describe the population experiencing post-COVID-19 sequelae within a primary care setting. By characterising demographic, clinical, and symptomatic features these patients, seeks enhance understanding long COVID in context first-level healthcare. Study Design: A single-centre, retrospective, cross-sectional was conducted. Place Duration Study: Family Medicine Unit. conducted from January 1st, 2023, March 31st, 2025, with COVID-19 Mexican patients attending...
The alphaviruses are composed of two icosahedral protein shells, one nested within the other. A membrane bilayer derived from host cell is sandwiched between shells. shells attached to another by domains which extend proteins outer shell through attach inner shell. We have examined interaction membrane-spanning domain glycoproteins with and other virus in an attempt understand role this plays assembly function. Through incremental deletions, we reduced length a transmembrane its normal 26...