Exosomes (Exos) secreted by adipose-derived stem cells (ADSCs) have shown potential in alleviating osteoarthritis (OA). Previous studies indicated that infrapatellar fat pad (IPFP) derived (IPFSCs) may be more suitable for the treatment of OA than subcutaneous adipose tissue (ScAT) (ScASCs). However, it remains unclear which type Exos offers superior therapeutic benefit OA. This study first compared differences between from IPFP (ExosIPFP) and ScAT (ExosScAT) treatment. Results suggested...

Abstract The study investigated whether both the osteogenic and angiogenic potential of Exos (Exosomes) can be enhanced by overexpression exosomal miRNA (microRNA) to confirm loaded in HMPs (Hydrogel microparticles) exert long‐term effects during new bone formation. BMSCs are successfully obtained. In vitro vivo experiments confirmed that HDAC4 (Histone deacetylase 4) is inhibited miR‐29a accompanied upregulation RUNX2 (Runt‐related transcription factor 2) VEGF (Vascular Endothelial Growth...

Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degeneration. Chondrocyte inflammation, apoptosis, and extracellular matrix degradation accelerated OA progression. MicroRNA (miRNA) has the potential to be therapeutic method for osteoarthritis. However, it difficult penetrate cell exercise its biological function, effect unclear. lipo-AgPEI-miR-200c-3p was created combining miR-200c-3p with silver nitrate polyvinylimine nanoparticles on microfluidic...

The objective of this study was to explore the effects intermittent hydrostatic pressure (IHP) on chondrogenic differentiation cartilage progenitor cells (CPCs) cultivated in alginate beads. CPCs were isolated from knee joint rabbits, and infrapatellar fat pad-derived stem (FPSCs) chondrocytes (CCs) included as control cell types. Cells embedded beads treated with IHP at 5 Mpa 0.5 Hz for 4 h/day 1, 2, or weeks. cells' migratory proliferative capacities evaluated using scratch Live/Dead...

Objectives The aim of this study was to detect levels common lipid species in serum and synovial fluid (SF) primary knee osteoarthritis (OA) patients investigate their correlations with disease severity. Materials Methods enrolled 184 OA receiving arthroscopic debridement or total arthroplasty 180 healthy controls between April 2012 March 2018. Total triglycerides (TG), cholesterol (TC), high-density lipoprotein (HDL-C), low-density (LDL-C), apolipoprotein A1 (ApoA1), B (ApoB) were analyzed...

Objective The purpose of this study was to determine whether the platelet-rich plasma–agarose gel scaffold could be a bioactive capable growth factors release for cartilage repair. Methods Porcine chondrocytes were seeded in agarose and gel. During 28-days culture, microstructure hydrogels morphologies observed using scanning electron microscope; viability gels examined by live/dead assay; qualitative quantitative analysis glycosaminoglycan, collagen DNA assessed histological,...

This study aimed to determine the effect of fibronectin (FN) on cartilage regeneration through activation chondrogenic progenitor cells (CPCs). Cells were isolated from knee mice and cultured in presence various concentrations FN. Proliferation, migration, differentiation assays performed vitro. In some experiments, CPCs preincubated with anti-integrin α5β1 antibody for 60 min before FN treatment block integrin receptor. Soluble was mixed Pluronic F-127 injected into joint cavity an...

Abstract Background Osteoarthritis (OA) is a major cause of limb dysfunction, and distraction arthroplasty which generates intermittent hydrostatic pressure (IHP) an effective approach for OA treatment. However, the result was not always satisfactory reasons remained unresolved. Because aging recognized as important risk factor chondrogenic progenitor cells (CPCs) could acquire senescent phenotype, we made hypothesis that CPCs senescence have harmful effect on chondrogenesis outcome be...

Three-dimensional (3D) printing technology, has achieved good results in articular cartilage damage repair, yet regeneration of hyaline similar to native and effectively performing clinical transformation remain challenging. In this study, we used 3D bioprinting technology add chondrogenic progenitor cells (CPCs) fibronectin (FN) an alginate/gelatin/hyaluronic acid (Alg/Gel/HA) composite hydrogel. We hydrogel prepare active biofilm with uniform pores using modified autologous matrix-induced...

Abstract The aim of this study was to assess the feasibility creating extracellular matrix (ECM) scaffolds from mesenchymal stem cells. Bone marrow cell (BMSC)‐derived ECM (BMSC‐dECM) were fabricated by lyophilization after crosslinking, without using a decellularization process. Acellular porcine chondrocyte‐derived (AC‐dECM) used as control. surface morphology, internal structure, water uptake ratio, mechanical properties, and biocompatibility scaffolds, well in vitro behavior cells grown...

Bone marrow stimulation treatment by bone stromal cells (BMSCs) released from the medullary cavity and differentiated into cartilage via microfracture surgery is a frequently employed technique for treating articular injuries, yet presents main drawback of poor regeneration in elderly. Prior research indicated that aging could decrease stemness capacity BMSCs, thus we made hypothesis increasing old BMSCs (OBMSCs) might improve results First, investigated correlation between outcomes using...

To develop a hybrid scaffold that enhances bone regeneration and preventing infections by integrating polycaprolactone (PCL) scaffolds for mechanical support with bacterial cellulose (BC) membranes psoralen (Pso) delivery,aiming to improve open defect treatment. PCL were fabricated via 3D printing, BC was extracted from fungi prepare three types of scaffolds: PCL, BC-PCL, BC-Pso-PCL. The characterized through scanning electron microscopy (SEM), contact angle measurement, infrared...

To determine the effects of cartilage progenitor cells, bone marrow mesenchymal stem cells and chondrocytes on repair as seed cells.Porcine (CPCs), (BMSCs) (CCs) were obtained from femoropatellar joints young pigs, seeded in agarose gel a graft. During 28-day culture, proliferation ability was measured by MTT assay, gene expression Collagen I, II, Aggrecan SOX 9 qPCR. Qualitative quantitative analysis collagen, glycosaminoglycan DNA appraised immunohistochemical staining biochemical...

To determine the signal transduction pathways involved in chondrocyte migration and their effects on cartilage integration autologous implantation. Articular chondrocytes were divided into three inhibitor groups pretreated with different inhibitors to Src, phospholipase Cγ1 (PLCγ1), extracellular signal-regulated kinase (ERK)1/2 signaling one control group vehicle. The effect of these was first explored by Boyden chamber assay, then an vitro cell/ring model. Chondrocyte visualized quantified...

The successful surgical restoration of damaged meniscus has been a challenge, largely owing to lack characterization cells and their precursors. Numerous strategies repair or replace have achieved only limited success. Several recent studies shown beneficial effect mesenchymal stem in repair. objective our study was characterize derived terms of, colony formation, proliferation, multi potency self-renewal capacity.Mesenchymal were isolated from menisci, patellar tendon bone marrow rabbits....

Long-chain noncoding small nucleolar RNA host gene 14 (LncRNA SNHG14) is highly expressed in various diseases and promotes progression, but the role mechanism of LncRNA SNHG14 on targeting miR-137 promoting osteoarthritis (OA) chondrocyte injury remains unclear. To measure expression LncRNAs miR-137, cell survival, inflammatory response, apoptosis, extracellular matrix (ECM) levels, we subjected human chondrocytes to a variety lipopolysaccharide (LPS) concentrations. luciferase activity...

ABSTRACT Tissue engineering for articular cartilage repair has shown success in ensuring the integration of neocartilage with surrounding natural tissue, but rapid restoration biomechanical functions remains a significant challenge. The poly(vinyl alcohol) (PVA) hydrogel is regarded as potential replacement its fair mechanical strength, whereas lack bioactivity limits utility. To obtain scaffold possessing expected and initial properties, we herein report novel salt‐leaching technique to...

Rotavirus is the most common cause of gastroenteritis in children up to five years age worldwide. The aim present study was analyze genotypes rotavirus strains isolated from with gastroenteritis, after introduction vaccine México. detected 14/100 (14%) fecal samples using a commercial test kit. viral genome purified these and used as template RT-PCR amplification VP4 VP7 genes, followed by gene cloning sequencing. Among strains, 4/14 (28.5%) were characterized G12P[8], 2/14 (14.3%), G12P...

To investigate the short-term effectiveness of joint distraction by Ilizarov combined with arthroscopic debridement in treatment knee osteoarthritis (KOA).Between January 2014 and 2015, 15 patients (15 knees) KOA were treated using assisting technology. There 7 males 8 females, aged from 45 to 64 years (mean, 55 years). The left right involved 6 9 cases respectively. disease duration was 2.0-9.5 (median, They all had received conservative for months got poor clinical improvement....