A5072877921- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- IL-33, ST2, and ILC Pathways
- Systemic Lupus Erythematosus Research
- Polyomavirus and related diseases
- Neurobiology and Insect Physiology Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- CAR-T cell therapy research
- Stress Responses and Cortisol
- Ion Channels and Receptors
- Sarcoidosis and Beryllium Toxicity Research
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune Neurological Disorders and Treatments
- Pregnancy and Medication Impact
- Cytomegalovirus and herpesvirus research
- Pluripotent Stem Cells Research
- Metabolomics and Mass Spectrometry Studies
- Atherosclerosis and Cardiovascular Diseases
- Ion channel regulation and function
- Renal Diseases and Glomerulopathies
- Traditional Chinese Medicine Studies
Universität Hamburg
2012-2024
University Medical Center Hamburg-Eppendorf
2012-2024
Klinik und Poliklinik für Neurologie
2012-2021
Institute of Neuroimmunology of the Slovak Academy of Sciences
2018
University of Münster
2007
Recent findings indicate a pathogenic involvement of IL‐17‐producing CD8 + T cells in multiple sclerosis (MS). IL‐17 production has been attributed to subset that belong the mucosal‐associated invariant (MAIT) cell population. Here, we report reduction MAIT blood MS patients compared with healthy individuals, which significantly correlated IL‐18 serum levels patients. In vitro stimulation peripheral mononuclear from individuals and specifically activated cells. Moreover, together T‐cell...
BackgroundMultiple sclerosis (MS), an autoimmune disease of the central nervous system (CNS), can be suppressed in its early stages but eventually becomes clinically progressive and unresponsive to therapy. Here, we investigate whether therapeutic resistance MS attributed chronic immune cell accumulation behind blood-brain barrier (BBB).MethodsWe systematically track CNS-homing cells peripheral blood 31 patients matched healthy individuals integrated analysis 497,705 single-cell...
While a link between inflammation and the development of neuropsychiatric disorders, including major depressive disorder (MDD) is supported by growing body evidence, little known about contribution aberrant adaptive immunity in this context. Here, we conducted in-depth characterization T cell phenotype receptor (TCR) repertoire MDD. For cross-sectional case-control study, recruited antidepressant-free patients with MDD without any somatic or psychiatric comorbidities (n = 20), who were...
Abstract Multiple sclerosis (MS) is a T cell–driven inflammatory disease of the CNS. Research on cell subsets involved in MS pathogenesis has mainly focused classical CD4+ cells, especially Th17 as they produce proinflammatory, MS-associated cytokine IL-17. However, abundant unconventional mucosal-associated invariant (MAIT) cells are also able to MAIT characterized by high CD161 expression and semi-invariant Vα7.2 TCR, with which recognize bacterial yeast Ags derived from riboflavin...
Primary progressive multiple sclerosis (PPMS) shows highly variable disease progression with poor prognosis and a characteristic accumulation of disabilities in patients. These hallmarks PPMS make it difficult to diagnose currently impossible efficiently treat. This study aimed identify plasma metabolite profiles that allow diagnosis its differentiation from the relapsing-remitting subtype (RRMS), primary neurodegenerative (Parkinson´s (PD)) healthy controls (HC) significantly change during...
Skin-migratory dendritic cells (migDCs) are pivotal antigen-presenting that continuously transport antigens to draining lymph nodes and regulate immune responses. However, identification of migDCs is complicated by the lack distinguishing markers, it remains unclear which molecules determine their migratory capacity during inflammation. We show that, in skin, neuronal plasticity molecule activity-regulated cytoskeleton-associated protein/activity-regulated gene 3.1 (Arc/Arg3.1) was strictly...
Abstract Mucosal-associated invariant T (MAIT) cells have been implicated in various inflammatory diseases of barrier organs, but so far, their role kidney disease is unclear. Here we report that MAIT recognize prototypical ligand, the vitamin B2 intermediate 5-OP-RU presented by MR1, reside human and mouse kidneys. Single cell RNAseq analysis reveals several intrarenal subsets, one, carrying genetic fingerprint tissue-resident MAIT17 cells, activated expanded a murine model crescentic...
Differences in immune responses between women and men are leading to a strong sex bias the incidence of autoimmune diseases that predominantly affect women, such as multiple sclerosis (MS). MS manifests more than twice many making one most important risk factor. However, it is incompletely understood which genes contribute differences incidence. To address that, we conducted gene expression analysis female male human spleen identified transmembrane protein CD99 significantly differentially...
Abstract Mucosal-associated invariant T (MAIT) cells express semi-invariant cell receptors (TCR) for recognizing bacterial and yeast antigens derived from riboflavin metabolites presented on the non-polymorphic MHC class I-related protein 1 (MR1). Neuroinflammation in multiple sclerosis (MS) is likely initiated by autoreactive perpetuated infiltration of additional immune cells, but precise role MAIT MS pathogenesis remains unknown. Here, we use experimental autoimmune encephalomyelitis...
Recent association studies have linked numerous genetic variants with an increased risk for multiple sclerosis, although their functional relevance remains largely unknown. Here we investigated phenotypical and consequences of a variant in the CD226 gene that, among other autoimmune diseases, predisposes to sclerosis. Phenotypically, effector regulatory CD4(+) memory T cells healthy individuals carrying predisposing showed, comparison carriers protective variant, reduced surface expression...
Identifying T cell clones associated with human autoimmunity has remained challenging. Intriguingly, many autoimmune diseases, including multiple sclerosis (MS), show strongly diminished activity during pregnancy, providing a unique research paradigm to explore dynamics of immune repertoire changes active and inactive disease. Here, we characterize immunomodulation at the single-clone level by sequencing in healthy women female MS patients over course pregnancy. Clonality is significantly...
Abstract The embryonic stem cell test (EST) represents the only validated and accepted in vitro system for detection classification of compounds according to their developmental reproductive teratogenic potency. widespread implementation EST, however, particular routine application pharmaceutical development, has not been achieved so far. Several drawbacks still limit high-throughput screening potential drug candidates this format: long assay period, use non-homogeneous viability assays, low...
Although most of the progressive multifocal leukoencephalopathy cases in sarcoidosis patients are explained by treatment with immunosuppressive drugs, it is also reported treatment-naive patients, which implies a general predisposition for leukoencephalopathy. Indeed, was shown that active have increased regulatory T cell frequencies could lead to subsequent systemic immunosuppression. However, if changes subsets constitute risk factor development leukoencephalopathy, then be counteracted...
Neurodegeneration in central nervous system disorders is linked to dysregulated neuronal calcium. Direct inhibition of glutamate-induced calcium influx, particularly via N-methyl-D-aspartate receptors (NMDAR), has led adverse effects and clinical trial failures. A more feasible approach modulate NMDAR activity or signaling indirectly. In this respect, the calcium-activated non-selective cation channel transient receptor potential melastatin 4 (TRPM4) been identified as a promising target....
Amiloride-sensitive Na + absorption is a well-described feature of numerous transporting epithelia in vertebrates. Yet, very little known about this important physiological process regarding invertebrates. In the present paper, we compare vertebrate mediated by amiloride-sensitive epithelial channel (ENaC) and its invertebrate counterpart. We used dorsal skin annelid Hirudo medicinalis as model for epithelia. applying electrophysiological, molecular, biochemical techniques found striking...