A5042368134- 3D Printing in Biomedical Research
- Click Chemistry and Applications
- Neuroscience and Neural Engineering
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Advanced Sensor and Energy Harvesting Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biotin and Related Studies
- Pluripotent Stem Cells Research
- Trypanosoma species research and implications
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Evolution and Genetic Dynamics
- Chemical Synthesis and Reactions
- Tissue Engineering and Regenerative Medicine
- Microbial Community Ecology and Physiology
- Additive Manufacturing and 3D Printing Technologies
- Anatomy and Medical Technology
- Electrospun Nanofibers in Biomedical Applications
- Asymmetric Synthesis and Catalysis
- Cancer, Stress, Anesthesia, and Immune Response
- Photochromic and Fluorescence Chemistry
- Chemical synthesis and alkaloids
- Image Processing Techniques and Applications
University of Oxford
2014-2025
Ludwig Cancer Research
2020-2025
University of St Andrews
2012-2013
Chinese Academy of Sciences
2008
Shanghai Institute of Materia Medica
2008
Abstract Bio-integrated devices need power sources to operate 1,2 . Despite widely used technologies that can provide large-scale targets, such as wired energy supplies from batteries or wireless transduction 3 , a efficiently stimulate cells and tissues on the microscale is still pressing. The ideal miniaturized source should be biocompatible, mechanically flexible able generate an ionic current for biological stimulation, instead of using electron flow in conventional electronic 4–6 One...
Bacteria often live in diverse communities where the spatial arrangement of strains and species is considered critical for their ecology. However, a test this hypothesis requires manipulation at fine scales which structure naturally occurs. Here we develop droplet-based printing method to arrange bacterial genotypes across sub-millimetre array. We print gut bacterium Escherichia coli that compete with one another using protein toxins. Our experiments reveal toxin-producing largely eliminate...
Human mutations in neuropeptide Y (NPY) have been linked to high body mass index but not altered dietary patterns
Advances in the development of tiny devices with sizes below a few cubic millimeters require corresponding decrease volume driving power sources. To be minimally invasive, prospective sources biomedical must fabricated from soft materials. Previous endeavors droplet-based have produced promising miniature sources; however, rechargeable battery has remained out reach. Here we report microscale flexible lithium-ion droplet (LiDB) based on lipid-supported assembly droplets constructed...
Abstract Current understanding of human brain development is rudimentary due to suboptimal in vitro and animal models. In particular, how initial cell positions impact subsequent cortical unclear because experimental spatial control arrangement technically challenging. 3D printing provides a rapid customized approach for patterning. However, it has relied on materials that do not represent the extracellular matrix (ECM) tissue. Therefore, present work, lipid‐bilayer‐supported technique...
Engineering human tissue with diverse cell types and architectures remains challenging. The cerebral cortex, which has a layered cellular architecture composed of layer-specific neurons organised into vertical columns, delivers higher cognition through intricately wired neural circuits. However, current engineering approaches cannot produce such structures. Here, we use droplet printing technique to fabricate tissues comprising simplified cortical columns. Human induced pluripotent stem...
Understanding how drugs work in vivo is critical for drug design and maximizing the potential of currently available drugs. 5-nitrofurans are a class prodrugs widely used to treat bacterial trypanosome infections, but despite relative specificity, often cause serious toxic side effects people. Here, we use yeast zebrafish, as well human vitro systems, assess biological activity 5-nitrofurans, identify conserved interaction between aldehyde dehydrogenase (ALDH) 2 across these species. In...
Recently, the World Health Organization approved nifurtimox–eflornithine combination therapy for treatment of human African trypanosomiasis, renewing interest in nitroheterocycle therapies this and associated diseases. In study, we have synthesized a series novel 5-nitro-2-furancarboxylamides that show potent trypanocidal activity, ∼1000-fold more than nifurtimox against vitro Trypanosoma brucei with very low cytotoxicity HeLa cells. More importantly, most analogue showed limited...
Abstract One of the open challenges in chemical biology is to identify reactions that proceed with large rate constants at neutral pH values. As shown here, dialdehydes react O ‐alkylhydroxylamines rates 500 M −1 s values absence catalysts. The key these conjugations an unusually stable cyclic intermediate, which ultimately undergoes dehydration yield oxime. scope and limitations method are outlined, as well its application bioconjugation a mechanistic interpretation will facilitate further...
The poor survival of ovarian cancer patients is linked to their high likelihood relapse. In spite full apparent macroscopic clearance, tumor recurrences arise from cells that are resistant primary chemotherapy in the form minimal residual disease (MRD). MRD exhibits distinct molecular drivers bulk and therefore necessitates alternative therapeutic strategies. However, there a lack 3D models faithfully recapitulate ex vivo for therapy development. This study constructs microfluidics-based...
Abstract Synthetic cells, such as giant unilamellar vesicles, can be engineered to detect and release chemical signals control target cell behavior. However, over target‐cell populations is limited due poor spatial or temporal resolution the inability of synthetic cells deliver patterned signals. Here, 3D‐printed picoliter droplet networks are described that direct gene expression in underlying bacterial by a signal with control. Shrinkage prior use achieves ≈50 µm resolution. Ways store...
A concise synthesis of melohenine B and O-ethyl-14-epimelohenine B, from eburnamonine, was achieved via a biomimetic diastereoselective singlet oxygen-mediated oxidative cleavage the indole C2–C7 bond. These studies enabled assignment absolute configuration natural products. In line with proposed biosynthetic pathway, resulting nine-membered ring containing products could be converted to corresponding quinolones.
We report a single-molecule mechanistic investigation into 2-cyanobenzothiazole (CBT) chemistry within protein nanoreactor. When simple thiols reacted reversibly with CBT, the thioimidate monoadduct was approximately 80-fold longer-lived than tetrahedral bisadduct, important implications for design of molecular walkers. Irreversible condensation between CBT derivatives and N-terminal cysteine residues has been established as biocompatible reaction site-selective biomolecular labeling...
Abstract Enzyme‐enabled biobatteries are promising green options to power the next‐generation of bioelectronics and implantable medical devices. However, existing sources based on enzymatic biofuel chemistry exhibit limited scale‐down feasibility due solid bulky battery structures. Therefore, miniature soft alternatives needed for integration with implants tissues. Here, a biobattery built from nanolitre droplets, fuelled by enzyme‐enabled oxidation reduced nicotinamide adenine dinucleotide,...
Abstract Bioengineered gastrointestinal (GI) tracts have potential applications in regenerative medicine and disease modeling. Methods for engineering tubular GI containing natural extracellular matrix (ECM) are currently limited. Here, the fabrication of collagen tubes with designed shapes by using lipid bilayer supported droplet networks is reported. Droplets cells arrayed lipid‐containing oil to form networks, which undergo thermal gelation provide continuous tubes. A variety tissues...
Abstract One of the open challenges in chemical biology is to identify reactions that proceed with large rate constants at neutral pH values. As shown here, dialdehydes react O ‐alkylhydroxylamines rates 500 M −1 s values absence catalysts. The key these conjugations an unusually stable cyclic intermediate, which ultimately undergoes dehydration yield oxime. scope and limitations method are outlined, as well its application bioconjugation a mechanistic interpretation will facilitate further...
Abstract Bacteria often live in diverse communities where the spatial arrangement of strains and species is considered critical for their ecology, including whether can coexist, which are ecologically dominant, how productive they as a community 1,2 . However, test importance structure requires manipulation at fine scales this naturally occurs 3–8 Here we develop droplet-based printing method to arrange different bacterial genotypes across sub-millimetre array. We use fine-scale by gut...
Abstract We report a single‐molecule mechanistic investigation into 2‐cyanobenzothiazole (CBT) chemistry within protein nanoreactor. When simple thiols reacted reversibly with CBT, the thioimidate monoadduct was approximately 80‐fold longer‐lived than tetrahedral bisadduct, important implications for design of molecular walkers. Irreversible condensation between CBT derivatives and N‐terminal cysteine residues has been established as biocompatible reaction site‐selective biomolecular...
Abstract Enzyme‐enabled biobatteries are promising green options to power the next‐generation of bioelectronics and implantable medical devices. However, existing sources based on enzymatic biofuel chemistry exhibit limited scale‐down feasibility due solid bulky battery structures. Therefore, miniature soft alternatives needed for integration with implants tissues. Here, a biobattery built from nanolitre droplets, fuelled by enzyme‐enabled oxidation reduced nicotinamide adenine dinucleotide,...