A5100430742- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Genomics and Phylogenetic Studies
- Cancer, Hypoxia, and Metabolism
- Bacillus and Francisella bacterial research
- Genomics and Rare Diseases
- Epilepsy research and treatment
- Optical Coherence Tomography Applications
- Caveolin-1 and cellular processes
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Poxvirus research and outbreaks
- Plant biochemistry and biosynthesis
- Bioinformatics and Genomic Networks
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- Silicon Effects in Agriculture
- Plant nutrient uptake and metabolism
- Extracellular vesicles in disease
- Gene expression and cancer classification
- MicroRNA in disease regulation
- Microbial Community Ecology and Physiology
University of Oxford
2023-2025
École Polytechnique Fédérale de Lausanne
2025
Shanxi Agricultural University
2023-2024
West China Hospital of Sichuan University
2024
Guangdong Ocean University
2024
Sichuan University
2020-2024
California University of Pennsylvania
2024
Chinese Academy of Inspection and Quarantine
2020-2023
Hebei University
2021-2023
Tsinghua University
2021-2023
Objective Epilepsy and intellectual/developmental disabilities (ID/DD) have a high rate of co-occurrence. Here, we investigated gene mutations in Chinese children with unexplained epilepsy ID/DD. Methods We used targeted next-generation sequencing to detect within 300 genes related ID/DD 253 A series filtering criteria was find the possible pathogenic variations. Validation parental origin analyses were performed by Sanger sequencing. reviewed phenotypes patients each mutated gene. Results...
Abstract We performed next generation sequencing on 1696 patients with epilepsy and intellectual disability using a gene panel 480 epilepsy-related genes including all GABAA receptor subunit (GABRs), we identified six de novo GABR mutations, two novel GABRA5 mutations (c.880G>T, p.V294F c.1238C>T, p.S413F), GABRA1 (c.778C>T, p.P260S c.887T>C, p.L296S/c.944G>T, p.W315L) known (c.335G>A, p.R112Q c.343A>G, p.N115D) in intractable early onset epileptic encephalopathy. The...
MHA scaffolds promoted osteoblast proliferation <italic>via</italic> altering the osteoclast-derived exosomal cargo and decreasing efficiency of exosome uptake by osteoblasts.
The poor survival of ovarian cancer patients is linked to their high likelihood relapse. In spite full apparent macroscopic clearance, tumor recurrences arise from cells that are resistant primary chemotherapy in the form minimal residual disease (MRD). MRD exhibits distinct molecular drivers bulk and therefore necessitates alternative therapeutic strategies. However, there a lack 3D models faithfully recapitulate ex vivo for therapy development. This study constructs microfluidics-based...
Abstract We identified a de novo missense mutation, P302L, in the γ-aminobutyric acid type A (GABA ) receptor γ2 subunit gene GABRG2 patient with Dravet syndrome using targeted next-generation sequencing. The mutation was cytoplasmic portion of transmembrane segment M2 that faces pore lumen. GABA α1 and β3 subunits were coexpressed wild-type (wt) γ2L or mutant γ2L(P302L) HEK 293T cells cultured mouse cortical neurons. measured currents whole-cell single-channel patch clamp techniques,...
Abstract GABA A receptors are brain inhibitory chloride ion channels. Here we show functional analyses and structural simulations for three de novo missense mutations in the receptor β3 subunit gene ( GABRB3 ) identified patients with early-onset epileptic encephalopathy (EOEE) profound developmental delay. We sought to obtain insights into molecular mechanisms that might link defects biophysics biogenesis EOEE. The mutant residues part of conserved domains such as Cys-loop (L170R) M2-M3...
Our study revealed a novel mechanism in the CaA-attenuated angiogenic ability HCC cells possibly<italic>via</italic>reducing JNK-1-mediated HIF-1α stabilization and inducing ubiquitination-mediated HIF1α degradation.
Rapid multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopy in the high-wavenumber (HW) region shows great advantages real-time dynamic process visualizations, clinical diagnosis, abundant microplastic assessment, etc. Fourier transform CARS (FT-CARS) improves acquisition speed of to order tens kHz, yet typical approaches utilize intrinsic coherence ultrabroadband pulses notably impede attainable vibrations HW region. Here, a novel delay-spectral focusing dual-comb (DC) scheme...
Resuscitation promoting factors (Rpf), a class of proteins secreted by gram-positive bacteria including actinobacteria, promote the resuscitation dormant and spore germination. Here, we describe reconstitution activity Rpf protein from Nocardiopsis halophila CGMCC 4.1195Tin vitro in vivo. The was expressed host Escherichia coli BL21 codon plus (DE3) confirmed to have significant effect on viable but non-culturable (VBNC) N. halophila. Subsequently, rpf gene knocked out. We found that growth...
The clinical benefit of immunotherapy has been limited to a small subset patients with cancer. Several trials immune checkpoint inhibitors in multiple cancers have shown some improvement obese patients. However, how obesity regulates the microenvironment remains unclear.Bioinformatic analysis was used discover microenvironmental-related genes associated body mass index (BMI). expression ICOS tumor tissues detected using western blot, immunohistochemistry, quantitative real-time polymerase...
The cytochrome P450 monooxygenases (CYP450) are the largest enzyme family in plant metabolism and widely involved biosynthesis of primary secondary metabolites. Foxtail millet (Setaria italica (L.) P. Beauv) can respond to abiotic stress through a highly complex polygene regulatory network, which SiCYP450 is also involved. Although CYP450 superfamily has been systematically studied few species, research on foxtail not completed. In this study, three hundred thirty-one genes were identified...
Abstract Osteoporosis is a metabolic bone disease that affects hundreds of millions people worldwide and characterized by excessive loss protein mineral content. The incidence mortality osteoporosis increase with age, creating significant medical economic burden globally. importance cholesterol levels has been reported in the development diseases including osteoporosis. It important to note key enzymes molecules involved homeostasis are closely related formation. Excessive may cause...
In recognition of the misuse risks fentanyl, there is an urgent need to develop a useful and rapid analytical method detect monitor opioid drug. The surface-enhanced shifted excitation Raman difference spectroscopy (SE-SERDS) has been demonstrated suppress background interference enhance signals. this study, SE-SERDS was used for trace detection fentanyl in beverages. To prepare simulated illegal drug–beverages, fentanyls were dissolved into distilled water or Mizone as series test samples....
Understanding the completeness and accuracy of U.S. military anthrax vaccination data is important to design interpretation studies assess safety vaccine. We estimated agreement between electronically recorded in Defense Medical Surveillance System (DMSS) versus abstracted from hardcopy medical charts a representative sample 1998 2004.Medical chart abstractions were conducted at 28 treatment facilities for 4201 personnel. Abstracted 1817 personnel, representing 7400 vaccine doses, compared...
Caveolae play crucial roles in intracellular membrane trafficking and mechanosensation. In this study, we report that synaptotagmin-11 (Syt11), a synaptotagmin isoform associated with Parkinson's disease schizophrenia, regulates both caveolae-mediated endocytosis the caveolar response to mechanical stimuli astrocytes. Syt11-knockout (KO) accelerated endocytosis. Interestingly, structures on cell surface were markedly fewer absence of Syt11. Caveolar disassembly hypoosmotic astrocyte swelling...