A5070966519- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Radical Photochemical Reactions
- Synthesis and Catalytic Reactions
- Genomics and Chromatin Dynamics
- Protein Structure and Dynamics
- Crystallography and molecular interactions
- Catalytic C–H Functionalization Methods
- Cellular Mechanics and Interactions
- Genetic and Kidney Cyst Diseases
University of California, Berkeley
1999-2025
University of California, Los Angeles
2021
University of Washington
1999
Coronin is a highly conserved actin-associated protein that until now has had unknown biochemical activities. Using microtubule affinity chromatography, we coisolated actin and homologue of coronin, Crn1p, from Saccharomyces cerevisiae cell extracts. Crn1p an abundant component the cortical cytoskeleton binds to F-actin with high (Kd 6 × 10−9 M). promotes rapid barbed-end assembly filaments cross-links into bundles more complex networks, but does not stabilize them. Genetic analyses crn1Δ...
The mitotic spindle consists of a complex network proteins that segregates chromosomes in eukaryotes. To strengthen our understanding the molecular composition, organization, and regulation spindle, we performed system-wide two-hybrid screen on 94 implicated function Saccharomyces cerevisiae. We report 604 predominantly novel interactions were detected multiple screens, involving 303 distinct prey proteins. uncovered pattern extensive between reflecting intricate organization spindle....
Chromosome alignment on the mitotic spindle, also referred to as congression, is facilitated by translocation of side-bound chromosomes along microtubule surface, which allows establishment end-on attachment kinetochores plus ends. We use a reconstitution assay in lysates prepared from metaphase-arrested budding yeast show that kinetochore lateral surface microtubules dependent Stu2, homolog vertebrate XMAP215. Stu2 tracks both growing and shrinking ends but colocalizes with moving...
During mitosis, individual microtubules make attachments to chromosomes via a specialized protein complex called the kinetochore faithfully segregate daughter cells. Translocation of kinetochores on lateral surface microtubule has been proposed contribute high fidelity chromosome capture and alignment at mitotic midzone, but difficult observe in vivo because spatial temporal constraints. To overcome these barriers, we used total internal reflection fluorescence (TIRF) microscopy track...
Microtubules (MTs) are important for cellular structure, transport of cargoes and segregation chromosomes organelles during mitosis. The stochastic growth shrinkage MTs, known as dynamic instability, is necessary these functions. Previous studies to determine how individual MT-associated proteins (MAPs) affect MT dynamics have been performed either through in vivo studies, which provide limited opportunity observation MTs or manipulation conditions, vitro focus on purified proteins,...
The budding yeast Saccharomyces cerevisiae has a closed mitosis in which the mitotic spindle and cytoplasmic microtubules (MTs), both of generate forces order to faithfully segregate chromosomes, remain separated by nuclear envelope throughout cell cycle. Kar3, kinesin-14, distinct functions on MTs each compartment. Here, we show that two proteins, Cik1 Vik1, form heterodimers with regulate its localization function within along cycle-dependent manner. Using MT dynamics reconstitution assay...
During mitosis, individual microtubules make attachments to chromosomes via a specialized protein complex called the kinetochore faithfully segregate daughter cells. Translocation of kinetochores on lateral surface microtubule has been proposed contribute high fidelity chromosome capture and alignment at mitotic midzone, but difficult observe in vivo because spatial temporal constraints. To overcome these barriers, we used total internal reflection fluorescence (TIRF) microscopy track...
Faithful segregation of chromosomes into daughter cells during mitosis requires formation attachments between kinetochores and mitotic spindle microtubules. Chromosome alignment on the spindle, also referred to as congression, is facilitated by translocation side-bound along microtubule surface, which allows establishment end-on attachment plus ends. Spatial temporal constraints hinder observation these events in live cells. Therefore, we used our previously developed reconstitution assay...
ABSTRACT The budding yeast Saccharomyces cerevisiae has a closed mitosis in which the mitotic spindle and cytoplasmic microtubules (MTs) used to segregate chromosomes remain separated by nuclear envelope throughout cell cycle. Kar3, kinesin-14, unique roles both compartments been implicated capturing unattached kinetochores, stabilizing crosslinked interpolar (MT), creating intranuclear MT arrays at pole body for kinetochore capture karyogamy, respectively. Here, we show that two proteins,...
Through consecutive selective C–H functionalization at multiple sites, the direct molecular editing of heteroarene carbon-hydrogen (C–H) bonds has potential to grant rapid access into diverse space; a valuable but often challenging venture achieve in medicinal chemistry. Contrasting with electronically-biased heterocyclic bonds, remote benzocyclic on bicyclic aza-arenes are especially difficult differentiate due lack intrinsic steric/electronic biases. careful consideration distance and...